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排序方式: 共有231条查询结果,搜索用时 281 毫秒
141.
T. Sako A. Mori P. Lee H. Oda K. Saeki Y. Miki M. Kurishima K. Mimura S. Nozawa H. Mizutani Y. Makino K. Ishioka T. Arai 《Research in veterinary science》2011,90(3):396-403
Insulin detemir is the first member of a new class of long-acting soluble insulin analogues capable of maintaining the basal level of insulin in humans. In this preliminary study, we investigated the time-action profiles of insulin detemir in normal and diabetic dogs since the use of insulin detemir in canines has yet to be determined. Eight animals were used in our study (three normal and five insulin dependent diabetic dogs). Time-action profiles of insulin detemir were monitored in normal dogs using an artificial pancreas apparatus under euglycemic condition. Blood sampling was performed at 2 h intervals post feeding, with insulin administration, in insulin dependent diabetic dogs. Time-action profiles of insulin detemir, in normal dogs, demonstrated that insulin detemir is a long-lasting preparation similar to what has been observed in humans. A pronounced peak was detected at 8–10 h while the glucose-lowering effect lasted for over 24 h after insulin injection, thus illustrating its longer prolonged peak activity time. Furthermore, intensive glycemic control was achieved with insulin detemir in insulin dependent diabetic dogs, using a lower dosage than NPH insulin and insulin glargine therapeutic doses. Our results indicate that insulin detemir has a greater effect than either NPH insulin or insulin glargine in canines, requiring a lower dose than either insulin preparation. However, using insulin detemir also carries a higher risk of inducing hypoglycemia as compared to either NPH insulin or insulin glargine. 相似文献
142.
Ages of fossil bones were determined by electron spin resonance spectroscopy. The electron spin resonance signal is associated with lattice defects or trapped centers produced by natural radiation in the bones and gives a measure of the total dose of natural radiation, or the archeological dose. Archeological doses were determined for samples of known age from a variety of sites and used to estimate apparent average annual rates of natural radiation at the sites. The method has the advantage that the sample need not be ground or heated, and it should be useful for dating biological materials. 相似文献
143.
Yusuke Murahata Yuya Miki Yoshiaki Hikasa 《Canadian journal of veterinary research》2014,78(4):304-315
This study aimed to investigate and compare the antagonistic effects of atipamezole, yohimbine, and prazosin on xylazine-induced diuresis in clinically normal cats. Five cats were repeatedly used in each of the 9 groups. One group was not medicated. Cats in the other groups received 2 mg/kg BW xylazine intramuscularly, and saline (as the control); 160 μg/kg BW prazosin; or 40, 160, or 480 μg/kg BW atipamezole or yohimbine intravenously 0.5 h later. Urine and blood samples were collected 10 times over 8 h. Urine volume, pH, and specific gravity; plasma arginine vasopressin (AVP) concentration; and creatinine, osmolality, and electrolyte values in both urine and plasma were measured. Both atipamezole and yohimbine antagonized xylazine-induced diuresis, but prazosin did not. The antidiuretic effect of atipamezole was more potent than that of yohimbine but not dose-dependent, in contrast to the effect of yohimbine at the tested doses. Both atipamezole and yohimbine reversed xylazine-induced decreases in both urine specific gravity and osmolality, and the increase in free water clearance. Glomerular filtration rate, osmolar clearance, and plasma electrolyte concentrations were not significantly altered. Antidiuresis of either atipamezole or yohimbine was not related to the area under the curve for AVP concentration, although the highest dose of both atipamezole and yohimbine increased plasma AVP concentration initially and temporarily, suggesting that this may in part influence antidiuretic effects of both agents. The diuretic effect of xylazine in cats may be mediated by α2-adrenoceptors but not α1-adrenoceptors. Atipamezole and yohimbine can be used as antagonistic agents against xylazine-induced diuresis in clinically normal cats. 相似文献
144.
Keisuke KOZAI Takuo HOJO Shota TOKUYAMA Anna Z SZóSTEK Masashi TAKAHASHI Miki SAKATANI Yasuo NAMBO Dariusz J SKARZYNSKI Kiyoshi OKUDA 《The Journal of reproduction and development》2014,60(2):150-154
Regression of the corpus luteum (CL) is characterized by a decay in progesterone
(P4) production (functional luteolysis) and disappearance of luteal tissues
(structural luteolysis). In mares, structural luteolysis is thought to be caused by
apoptosis of luteal cells, but functional luteolysis is poorly understood.
20α-hydroxysteroid dehydrogenase (20α-HSD) catabolizes P4 into its biologically
inactive form, 20α-hydroxyprogesterone (20α-OHP). In mares, aldo-keto reductase (AKR)
1C23, which is a member of the AKR superfamily, has 20α-HSD activity. To clarify whether
AKR1C23 is associated with functional luteolysis in mares, we investigated the expression
of AKR1C23 in the CL in different luteal phases. The luteal P4 concentration
and levels of 3β-hydroxysteroid dehydrogenase (3β-HSD) mRNA were higher in the mid luteal
phase than in the late and regressed luteal phases (P<0.05), but the level of 3β-HSD
protein was higher in the late luteal phase than in the regressed luteal phase
(P<0.05). The luteal 20α-OHP concentration and the level of AKR1C23
mRNA were higher in the late luteal phase than in the early and mid luteal phases
(P<0.05), and the level of AKR1C23 protein was also highest in the late luteal phase.
Taken together, these findings suggest that metabolism of P4 by AKR1C23 is one
of the processes contributing to functional luteolysis in mares. 相似文献
145.
146.
Reproductive cycle of yellow sea bream <Emphasis Type="Italic">Dentex tumifrons</Emphasis> in Wakasa Bay,the Sea of Japan off central Honshu 总被引:2,自引:0,他引:2
Osamu?TominagaEmail author Miki?Inoue Miho?Kamata Tadahisa?Seikai 《Fisheries Science》2005,71(5):1069-1077
ABSTRACT: To determine the spawning season and reproductive cycle of the yellow sea bream, Dentex tumifrons , in Wakasa Bay, in the Sea of Japan off central Honshu, seasonal change in the gonadosomatic index ( GSI ) was examined and histological observations of both ovaries and testes were conducted. The female GSI gradually increased from May, maintained a high level between August and October and then rapidly decreased. The histological observations of the ovary showed that vitellogenesis began from May and developed until September. Spent fish began to be observed in October and all the fish collected in December were spent. There was only one peak in the seasonal change in GSI of males and females in Wakasa Bay. Thus, yellow sea bream spawn only in autumn in Wakasa Bay. Differences in the reproductive cycle and number of spawning seasons were found between the two different latitudinal areas, Wakasa Bay and the East China Sea (low latitudinal area) where the spawning seasons were spring and autumn. An increase in water temperature is an important factor to initiate gonadal maturation (recrudescence) and is terminated by the short day-length in Wakasa Bay. 相似文献
147.
Terumichi Kawahara Miki Kanouchi Yousuke Naniwa Masanori Koyago Takashi Numabe Keishi Mizutani Kentaro Tanemura Kenshiro Hara 《Animal Science Journal》2021,92(1):e13572
Aging is a major risk factor for spermatogenesis deterioration. However, the influence of age on spermatogenic stem cells and their progenitors in bulls is largely unknown. Here, we report age-related changes in undifferentiated and differentiating spermatogonia in Japanese Black cattle with nearly constant sperm output, by using spermatogonial markers. The numbers of differentiating spermatogonia and more differentiated spermatogenic cells were significantly decreased in aged bovine testes compared with those in young testes. In contrast, the number of undifferentiated spermatogonia was maintained, and their proliferative activity did not differ significantly between young and aged bovine testes. Although severe calcification was only observed to a small extent in aged testes, fewer Sertoli cells and interstitial fibrosis were observed in noncalcified testicular regions. These results suggest that, even in old bulls with nearly constant sperm output, testicular spermatogenic activity declined whereas undifferentiated spermatogonia numbers were maintained. Thus, we propose that undifferentiated spermatogonia may be resistant to age-related changes in bovine testes. Because undifferentiated spermatogonia may contain stem cell activity, our findings highlight the potential utility of undifferentiated spermatogonia as an agricultural resource to produce spermatozoa beyond the natural bovine lifetime through transplantation and in vitro spermatogenesis in future animal production. 相似文献
148.
Molecular analysis and physicochemical properties of electrophoretic variants of wild soybean Glycine soja storage proteins 总被引:4,自引:0,他引:4
Fukuda T Maruyama N Kanazawa A Abe J Shimamoto Y Hiemori M Tsuji H Tanisaka T Utsumi S 《Journal of agricultural and food chemistry》2005,53(9):3658-3665
Cultivated soybeans (Glycine max) are derived from wild soybeans (Glycine soja) and can be crossed with them to produce fertile offspring. The latter exhibit greater genetic variation than the former, suggesting a possibility that wild soybeans contain storage proteins with properties different from and better than those of cultivated soybeans. To identify a wild soybean suitable for breeding a new soybean cultivar, we analyzed seed proteins from 390 lines of wild soybeans by electrophoresis. We found some lines containing electrophoretic variants of glycinin and beta-conglycinin subunits: one line containing a small alpha' subunit of beta-conglycinin and two and five lines containing small A3 and large A4 polypeptides of glycinin, respectively. Beta-Conglycinin and glycinin containing such variant subunits exhibited solubility and emulsifying ability similar to those of the predominant types of wild and cultivated soybeans. Glycinins containing small A3 and large A4 gave a shoulder derived from the start of denaturation at a temperature 4 degrees C lower than that of glycinin from the predominant types of wild and cultivated soybeans, although their thermal denaturation midpoint temperatures were very similar to each other. Cloning and sequencing of the predominant and variant subunit cDNAs revealed that the small alpha' and the small A3 lacked 24 amino acid residues in the extension region and four amino acid residues in the hypervariable region, respectively, and that the large A4 did not have an insert corresponding to the difference in the electrophoretic mobility but Arg279 and Gln305 were replaced by glutamine and histidine, respectively, in the hypervariable region. These suggest that small differences even in the hypervariable region can affect the thermal stability, as well as the electrophoretic mobilities, of the proteins. 相似文献
149.
R Matsuda M Ishibashi M Uchiyama T Hiraoka H Hoshida Y Kunihiro H Miki Y Nishimoto K Inazu T Mizuno 《Journal of the Association of Official Analytical Chemists》1987,70(4):681-686
Determination of organic compounds present as contaminants in purified water is of great importance. Total organic carbon (TOC) may be a useful parameter for evaluating the purity of water. This paper describes and compares the analytical results for solutions of 12 model compounds obtained by 3 methods: total organic carbon (TOC) analysis, determination of potassium permanganate consumption, and UV spectroscopy. TOC analysis gave values corresponding to the expected amounts of organic carbon for all the model compounds, whereas the other 2 methods gave highly variable results. 相似文献
150.
Miyake Y Fukumoto S Okada M Sakaida K Nakamura Y Osawa T 《Journal of agricultural and food chemistry》2005,53(1):22-27
Sesamin and sesaminol triglucoside in sesame seeds are major lignans that display an abundance of biological activities. Although their antioxidative activity in vitro is weak, they have been reported to suppress oxidative stress in vivo. We investigated the production of new antioxidative lignans from sesame lignans by culturing with the genus Aspergillus to enhance the function of food materials. Media containing sesamin or sesaminol triglucoside increased antioxidative activity for DPPH radical scavenging by culturing with Aspergillus usamii mut. shirousamii RIB2503. The antioxidative lignans in sesamin medium were identified as sesamin 2,6-dicatechol and episesamin 2,6-dicatechol. Those in sesaminol triglucoside medium were identified as sesaminol 6-catechol and episesaminol 6-catechol, which are novel antioxidative lignans. It is suggested that they may exhibit higher antioxidative activity than sesamin and sesaminol triglucoside because they have the catechol functional moiety. 相似文献