粤优丝瓜的选育

粤优丝瓜是以汕头丝瓜自交系D2为母本,以雅岗丝瓜自交系S12为父本配制而成的一代杂种。早熟,植株生长势及分枝力强,坐果率高,果实棍棒形,匀称,皮色绿白,瓜纵径49.0cm、横径4.8cm,肉厚0.77cm,单瓜质量0.33kg,味清甜,每667m2产量2000~2500kg。抗枯萎病,高抗疫病,耐霜霉病,适宜广东省春秋种植。     

不同砧木嫁接网纹甜瓜试验

为预防网纹甜瓜枯萎病等土传病害,以新土佐、灰甘瓜、全能铁甲南瓜,甬砧3号甜瓜为砧木,以当地主栽的拿玻里网纹甜瓜为接穗,进行嫁接试验。结果表明:拿玻里网纹甜瓜嫁接后,植株的抗病性有了显著提高,对枯萎病的防效高达100%,对白粉病的防效也有所提高,综合抗性增强。其中甬砧3号在4个参试砧木品种中,与接穗拿玻里网纹甜瓜的亲和性最好,抗病性提高明显,品质也较自根苗好。     

八珍汤对乳牛产后免疫状态的影响

为了探索提高乳牛产后期免疫状态的新方法，对试验乳牛分4组（产前第30d至产前第1d灌喂组、产前第15d至产后第15d灌喂组、产后第1d至第30d灌喂组、不灌喂中药对照组）灌喂中药八珍汤，检测产后期淋巴细胞及其亚群数量和淋巴细胞增殖活化功能。结果发现，产前组CD3细胞数量在产后第1d升高；产前产后组CD3、CD4和CD8细胞数量在产后第1d和第15d升高；产后组CD3细胞数量在产后第15d和第30d升高，CD4细胞数量也在产后第30d升高。淋巴细胞对ConA的反应能力，产前产后组在产后第1～30d明显提高，产后组在产后第15d和第30d明显提高。各组乳牛产后期CD21细胞数量的变化相近。结果表明，从产前第15d开始到产后第15d每日喂八珍汤，能明显提高乳牛产后期T细胞及其亚群数量和增加淋巴细胞增殖活化功能，而对B细胞数量增加的作用不明显。     

Analysis of gene expression profile of multidrug resistant MCF/DOX cell line after benflumetol derivative LY980503 treatment

AIM:To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray.METHODS:Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile.RESULTS:9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503.CONCLUSION:LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.     

Effects of matrix metalloproteinases on ventricular remodelingfollowing myocardial ischemia in the rat

AIM:To examine the relationship between the activity of matrix metallproteinases(MMPs) and ventricular remodeling following myocardial ischemia in the rat.METHODS:The model of myocardial ischemia(MI) in the rat was established by isoprenaline(ISP). The activity of MMPs was measured by zymography and collagen concentration was assessed by the method of chloramine T, so did I/III collagen ratio by immunohistochemical staining. The microstructure of myocardium was also observed by electron microscope.RESULTS:The activity of MMP-2 in myocardial ischemia group (group M) increased by 5.8 folds at 1 st week(P＜0.01), 2.3 folds at 2 nd week(P＜0.01) and 1.7 flods at 4 th week(P＜0.05) compared with control group (group C) and MMP-9's activity in group M increased by 4.9 folds (P＜0.01), 1.9 flods(P＜0.01) and 1.4 folds(P＜0.05), respectively. Collagen amount and I/III collagen ratio in group M increased compared with that in group C at 2 nd week and 4 th week. It showed that cardiac myocytes in group M were necrosed and collagen grew abundantly in interstitium under electron microscope.CONCLUSION:The activity of MMPs in the myocardial interstitium increased following myocardial ischemia, then collagen amount and I/III collagen ratio increased, which may be the major causes of ventricular remodeling.     

The basic mechanism of increased microvascular permeability

The increased microvascular permeability appears mainly in venule during inflammation, shock, and burns. Endothelial cells play an important role in venule permeability enhancement. There are two kinds of pathway for macromolecule extravasation. One is paracellular pathway and another is transcellular pathway, which are related to the formation of endothelial gap or transcellular openings seperately. The alteration of intercellular related protein, such as occludin, claudin, zona occludens (ZO), junctional adhesion molecule (JAM), VE cadherin, catenin, integrin, etc, and the alteration of endothelial cytoskeleton, such as rearrangement of actin filament, formation of stress fiber and focal adhesion, etc, involve in the pathogenesis of increased microvascular permeability.     

中国风水侵蚀交错区分布特征分析

风水侵蚀交错带的研究 ,对景观、土地覆盖 /土地利用、全球变化等研究具有重要意义。论文以GIS技术为支撑 ,定量计算出我国风水侵蚀带。在分布特征上 ,主要分布我国北方地区 ,其中以西部沙漠 ,北部山脉沿线为重 ,这种分布与气候、地貌分界有明显联系。 49.6 %风水侵蚀复合带的降水量小于 2 0 0mm。风水侵蚀复合带的土地利用主要以草地为主 ,占 41.8% ,其次为耕地。两种类型的分布 ,草地以与其它土地利用类型交错分布为主 ;而耕地是有重心的分散分布。风水侵蚀复合带的总体侵蚀要强于全国水平。     

天然芸苔素内酯缓解胺苯磺隆对后茬水稻药害的作用机理初探

通过测定天然芸苔素内酯对水稻幼苗谷胱甘肽(GSH)含量、谷胱甘肽-S-转移酶(GST)活性以及在离体和活体条件下对水稻幼苗乙酰乳酸合成酶(ALS)活力的影响,研究天然芸苔素内酯减轻胺苯磺隆对水稻药害的作用机理。结果表明,其作用机理与水稻幼苗GSH含量、GST活性变化没有太大相关性,可能与其能间接激活水稻幼苗ALS活力有较大相关。     

Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione

AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO₂^-／NO₃^- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO₂^-／NO₃^- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.     

Effects of Sini decoction on atherosclerosis and ceramide content of aorta in rabbits

AIM:To observe the effects of Sini decoction on atherosclerosis(AS) and ceramide content of aorta in rabbits. METHODS:28 rabbits were randomly divided into three groups. Control group was fed with a normal diet; High cholesterol group was fed 1% cholesterol and 5% fat diet; Sini decotion+ high cholesterol group was fed 1% cholesterol and 5% fat diet plus Sini decotion (4.2 g·kg^-1·d^-1). At the end of study, the plaque area were measured, the atorta ceramide and cell apoptosis were also detected. RESULTS:Sini decotion diminished lipid plaque area on the aortic endothelium, reduced the levels of aorta ceramide and the apoptosis index. CONCLUSION:Sini decoction has an inhibitory effect on AS, the mechanism may be that Sini decotion reduces concentration of ceramide in aorta.