谷物培养基上白灵菇菌丝生长研究

本研究以白灵1号为供试菌株,通过对菌丝萌发时间、颜色、密度、均匀度、平均生长速率和生长指数进行比较,探讨7种不同谷物(脱壳与不脱壳)对白灵菇菌丝生长的影响。结果表明,无论脱壳与否,白灵1号在供试的7种谷物培养基上均能生长,但生长情况各不相同,其中最适合白灵菇菌丝生长的是脱壳高粱培养基,长势评分最好,为5分,平均生长速率最大,达5.892 mm·d-1,菌丝生长指数为29.44,其次为不脱壳的小麦和高粱培养基,最不适合白灵菇菌丝生长的是水稻培养基,长势评分为1分,菌丝生长指数为1.75。     

早熟甜油桃新品种‘秦光3号’

‘秦光3号’油桃是以‘82-4-33’为母本,‘阿姆肯’为父本,通过有性杂交培育而成的早熟新品种。果实生育期78 d。果实椭圆形,平均单果质量160 g,最大果245 g,果肉白色,果面着粉红色晕,外观漂亮,风味甜浓,芳香,可溶性固形物13.0%,品质优,半离核,丰产。     

Downregulation of DEK induces cell apoptosis via inhibition of NF-κB signaling pathway in gastric carcinoma SGC-7901 cells

AIM: To investigate the effect of DEK downregulation on the apoptosis of gastric carcinoma SGC-7901 cells, and to explore its associations with NF-κB signaling pathway and apoptosis related proteins. METHODS: SGC-7901 cells with different treatments were divided into 3 groups including untreated group, control siRNA group and DEK siRNA group. The expression of DEK at mRNA and protein levels in the SGC-7901 cells was detected by real-time PCR and Western blot. The cell apoptosis was examined by flow cytometry. Furthermore, the activities of caspase-3 and caspase-9 in the SGC-7901 cells were investigated by Caspase-Glo^®-3/9 kit. Finally, the expression of key regulatory protein p65 of NF-κB signaling pathway and apoptosis-related proteins Bcl-2 and Bax in the SGC-7901 cells was investigated by Western blot. RESULTS: Compared with untreated group and control siRNA group, the expression of DEK at mRNA and protein levels was significantly downregulated in DEK siRNA group (P<0.05). In addition, the ratios of early phase apoptosis and total apoptosis in DEK siRNA group were markedly higher than those in untreated group and control siRNA group (P<0.05). Most notably, the decrease in p65 and Bcl-2 proteins, increase in Bax protein and the increases of caspase-3 and caspase-9 activities were observed in DEK siRNA group. CONCLUSION: Downregulation of DEK mediates cell apoptosis of gastric carcinoma may be tightly associated with NF-κB signaling pathway.     

Effect of microRNA-155 on regulation of angiogenesis in diabetic rats with cerebral ischemic injury

AIM: To evaluate the effect of microRNA-155(miRNA-155) on the regulation of angiogenesis in diabetic rats with cerebral ischemic injury. METHODS: Adult male Sprague-Dawley rats were randomly divided into 5 groups:sham group, cerebral ischemia group, diabetic cerebral ischemia group, diabetic cerebral ischemia+miRNA-155 inhibitors group and diabetic cerebral ischemia+scramble group. Diabetes model was made by injection of streptozocin and permanent cerebral ischemic model was developed by suture-occluded method. The scores of neurological deficit and infarct volume were estimated at 24 h after cerebral ischemia. miRNA-155 level was detected by real-time polymerase chain reaction. The expression of platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) and vascular endothelial growth factor(VEGF) was detected by Western blotting. RESULTS: miRNA-155 inhibitor significantly reduced miRNA-155 levels in the ischemic cortex(P<0.05), improved the scores of neurological deficit, reduced infarction size and upregulated the levels of CD31 and VEGF(P<0.05). CONCLUSION: miRNA-155 has a critical role in the regulation of angiogenesis in diabetic rats with cerebral ischemia. Down-regulation of miRNA-155 using miRNA-155 inhibitor attenuates brain infarct injury in diabetic rats.     

海南省西甜瓜细菌性果斑病的发生与防治

<正>近年来细菌性果斑病在海南西瓜、甜瓜生产上发生严重,其原因不仅与当地气候条件、栽培管理不当等有关,也与各地进行南繁的瓜类品种繁杂、种子消毒不彻底、病原远距离传播扩散有关。西瓜、甜瓜是海南省种植面积较为广泛、经济效益较高的农产品,2013年种植面积达1.97万hm2(29.55万亩)(农业部,2015)。细菌性果斑病是西瓜、甜瓜生产上的一种毁灭性病害,引起该病的病原菌为西瓜嗜酸菌(Acidovorax citrulli)(Schaad     

豫西黄土丘陵区生态环境条件分析与生态果园复合模式研究

为了更好地在豫西黄土丘陵区建设生态果园,分析了该区气候、地貌、土地、植被及水土流失等生态环境条件,介绍了"草-牧-沼-窖-果"、"草-牧-果"和以旅游观光为主的3种生态果园复合模式。并对生态果园建设中果园的规划和布局、水土保持措施建设、保水耕作技术及生物防治技术进行了分析和探讨,以期为生态果园建设提供理论基础和生产指导。     

微波辅助法提取牛蒡叶粗多糖的工艺研究

以牛蒡叶为试材,采用微波辅助碱液提取法,通过单因素试验和正交实验考察了料液比、微波提取功率、提取时间、提取次数等因素对牛蒡叶多糖提取率的影响。结果表明:影响牛蒡叶多糖提取率因素从大到小依次为微波功率、微波提取时间、料液比。最佳提取条件为微波功率80W、浸提时间120s、料液比1∶30g/mL,在此工艺条件下,多糖提取率达36.12%。     

疏粒处理对“赤霞珠”葡萄果实含糖量及相关代谢酶活性的影响

以酿酒葡萄品种"赤霞珠"为试材,分析了不同疏粒量处理对始熟期后葡萄叶片内容物、果实大小、果实糖酸含量及果实酸性转化酶活性的影响。结果表明:从始熟期开始随着果实成熟,对照和处理的果实纵横径及糖含量均逐渐增加,而果实酸含量及叶片中可溶性糖含量均逐渐降低;与此同时,可溶性酸性转化酶活性都呈先降低后升高趋势而细胞壁酸性转化酶活性却持续升高。另一方面,在相同果实成熟期随疏粒量的增加,果实糖含量明显上升,酸含量明显下降,而可溶性酸性转化酶及细胞壁酸性转化酶的活性均升高。另外,疏粒处理对各时期果实纵横径、叶片叶绿素影响不大,但随疏粒量的增加,各时期叶片可溶性糖含量却逐渐降低。对果实酸性转化酶活性、叶片可溶性糖及果实含糖量进行相关性分析发现,细胞壁酸性转化酶活性与叶片可溶性糖含量呈显著负相关而与果实含糖量呈显著正相关,而且随着果实疏粒量的增加,相关性有升高趋势。总之,葡萄果实成熟期疏粒处理对葡萄果实中的酸性转化酶产生一定影响,尤其是细胞壁酸性转化酶,最终可能改变了葡萄树体的库源关系,增加了果实库强,使更多的碳水化合物分配到果实中。     

金盏花新品种“陇菊1号”的选育

为了促进金盏花产业的可持续发展,选育出了适宜西北内陆干旱区种植的金盏花新品种,从2002年开始选育的金盏花新品种"陇菊1号"(原代号FL-2)于2012年12月通过了甘肃省农作物品种审定委员会的认定。FL-2为典型的杂交品种,植株健壮,分枝数多,鲜花产量2 978.90~3 812.25kg/667m2,叶黄素含量11.09~13.85g/kg,色素产量为5.51~8.31kg/667m2,抗金盏花叶斑病,适于甘肃省沿黄灌区及河西地区种植。     

基于地统计学的棉花长势空间变异分析

采用地统计学的变异函数分析方法对我国2006年棉花长势空间变异特性进行了分析,结果表明,基于县域尺度的我国长势指标存在空间异质性.5月真叶数、6月真叶数、7月总果节数、9月成铃数的半方差值随间隔的变化很好的符合高斯理论模型的变化趋势,模型拟合的决定系数变化在0.472～0.856之间,8月成铃数则较好地符合球状理论模型,模型拟合的决定系数为0.879.空间自相关范围分别是 1644.25、1822.61、2293.59、2777.63、1195.56 km.5月份单株真叶数99.9%的空间变异是由于结构性因素的原因造成的,7月份的单株总果节数的96.7%的空间变异是由于结构性因素的原因造成的,6月单株真叶数、8月单株成铃数、9月单株成铃数的空间分布的结构比分别为0.61、0.929、0.869.