全文获取类型
收费全文 | 809篇 |
免费 | 46篇 |
专业分类
林业 | 67篇 |
农学 | 18篇 |
基础科学 | 8篇 |
202篇 | |
综合类 | 60篇 |
农作物 | 37篇 |
水产渔业 | 80篇 |
畜牧兽医 | 304篇 |
园艺 | 26篇 |
植物保护 | 53篇 |
出版年
2024年 | 1篇 |
2023年 | 10篇 |
2022年 | 14篇 |
2021年 | 19篇 |
2020年 | 21篇 |
2019年 | 24篇 |
2018年 | 35篇 |
2017年 | 27篇 |
2016年 | 28篇 |
2015年 | 30篇 |
2014年 | 30篇 |
2013年 | 47篇 |
2012年 | 47篇 |
2011年 | 63篇 |
2010年 | 47篇 |
2009年 | 47篇 |
2008年 | 56篇 |
2007年 | 53篇 |
2006年 | 42篇 |
2005年 | 42篇 |
2004年 | 35篇 |
2003年 | 33篇 |
2002年 | 27篇 |
2001年 | 7篇 |
2000年 | 11篇 |
1999年 | 6篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 5篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1977年 | 1篇 |
1964年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有855条查询结果,搜索用时 15 毫秒
121.
Lasse Dam Rasmussen Giovanni Savini Alessio Lorusso Anna Bellacicco Massimo Palmarini Marco Caporale Thomas Bruun Rasmussen Graham J Belsham Anette B?tner 《Veterinary research》2013,44(1):75
Transplacental transmission of bluetongue virus has been shown previously for the North European strain of serotype 8 (BTV-8) and for tissue culture or chicken egg-adapted vaccine strains but not for field strains of other serotypes. In this study, pregnant ewes (6 per group) were inoculated with either field or rescued strains of BTV-2 and BTV-8 in order to determine the ability of these viruses to cross the placental barrier. The field BTV-2 and BTV-8 strains was passaged once in Culicoides KC cells and once in mammalian cells. All virus inoculated sheep became infected and seroconverted against the different BTV strains used in this study. BTV RNA was detectable in the blood of all but two ewes for over 28 days but infectious virus could only be detected in the blood for a much shorter period. Interestingly, transplacental transmission of BTV-2 (both field and rescued strains) was demonstrated at high efficiency (6 out of 13 lambs born to BTV-2 infected ewes) while only 1 lamb of 12 born to BTV-8 infected ewes showed evidence of in utero infection. In addition, evidence for horizontal transmission of BTV-2 between ewes was observed. As expected, the parental BTV-2 and BTV-8 viruses and the viruses rescued by reverse genetics showed very similar properties to each other. This study showed, for the first time, that transplacental transmission of BTV-2, which had been minimally passaged in cell culture, can occur; hence such transmission might be more frequent than previously thought. 相似文献
122.
Corti Martina Cavalli Daniele Cabassi Giovanni Vigoni Antonio Degano Luigi Marino Gallina Pietro 《Precision Agriculture》2019,20(4):675-696
Precision Agriculture - The development of small unmanned aerial vehicles and advances in sensor technology have made consumer digital cameras suitable for the remote sensing of vegetation. In this... 相似文献
123.
Alessandro Chiarucci Giovanni Bacaro Duccio Rocchini 《Biological conservation》2008,141(10):2608-2618
Assessing the effects of the spatial components on species diversity in a network of protected areas represents an important step for assessing its conservation “capacity”. A clear evaluation on how α-, β-, and γ-diversity are partitioned among and within spatial scales can help to drive manager decisions and provide method for monitoring species diversity. Moving from these concepts, a probabilistic sample of plant species composition was here applied for quantifying plant species diversity within the Sites of Community Importance (SCIs) of the Natura 2000 network in the Siena Province. All analyses were performed separately for all species and those species defined as “focal” (included in regional, national or continental “red” lists). The results indicated that species richness of the SCIs differed from one location to another one independently from the sampling efforts. Diversity partitioning indicated that most of the flora diversity within the network was given by larger-scale β-diversity, i.e. the differences in species composition among SCIs. β-diversity was then decomposed in two components: βArea (due to the differences in area among SCIs) and βReplacement (due to the compositional differences across SCIs). βArea was particularly important for all species, while βReplacement was the most important factor for focal species. The consequent implications for monitoring and nature conservation strategies are discussed. 相似文献
124.
Mazzotti F Di Donna L Maiuolo L Napoli A Salerno R Sajjad A Sindona G 《Journal of agricultural and food chemistry》2008,56(1):63-67
A high-throughput mass spectrometric method is presented for the simultaneous detection of Sudan I, II, III, IV, and Para-Red azodyes in foodstuff. The method is based on the use of deuterium-labeled internal standards and atmospheric pressure chemical ionization (APCI) in a triple-quadrupole instrument. The gas-phase breakdown pattern of each labeled and unlabeled analogue displays the naphthoic moiety as a common fragment. The search for the parents of this common species (parent ion scans) allows, by flow injection and in a single run, the evaluation of the presence of each polluting species spiked in typical foodstuffs. A detailed assay of each azodye was performed by LC-APCI and isotope dilution method, through the multiple reaction monitoring approach, using deuterium-labeled internal standards. Sudan dyes can be quantified above the threshold of 10 ppb except for Sudan Para-Red, for which the limit of quantification was 20 ppb, likely due to the different ionization efficiency. 相似文献
125.
García-Llatas G Cercaci L Rodriguez-Estrada MT Lagarda MJ Farré R Lercker G 《Journal of agricultural and food chemistry》2008,56(2):469-475
The effect of storage on sterol oxidation of ready-to-eat infant foods was evaluated. Two different liquid infant foods (honey or fruits flavors), prepared with milk and cereals, were stored for 0, 2, 4, 7 and 9 months at 25 degrees C. Sterol oxidation products (SOP) were isolated by cold saponification, purified by silica solid-phase extraction, and analyzed by gas chromatography (GC) and GC-mass spectrometry. beta-Sitosterol was the most representative sterol, followed by cholesterol and campesterol. No significant differences in the total and single SOP content (0.8-1 mg/kg of product) were observed with respect to storage time and type of sample; the main SOP found was 7-ketositosterol (<0.2 mg/kg of product). The extent of stigmasterol oxidation (2.9%) was higher than that of cholesterol (1.9%) and beta-sitosterol (1.4%). The type and quality of raw materials, as well as the processing conditions, seem to greatly influence SOP formation and accumulation in infant foods. 相似文献
126.
Bisio A Romussi G Russo E Cafaggi S Schito AM Repetto B De Tommasi N 《Journal of agricultural and food chemistry》2008,56(22):10468-10472
As a part of our search for biologically active compounds from cultivated Salvia spp. we investigated Salvia corrugata Vahl. The activity of two isolated icetaxane diterpene quinones, fruticuline A and demethylfruticuline A, was assessed against 46 bacterial pathogens, mostly resistant to several primary antibiotics. The MIC for all the inhibited Gram-positive pathogens tested showed a very narrow distribution and ranged from 32 to 64 mg/L, regardless of their resistance patterns to other antibiotics. Demethylfruticuline A was shown to be highly bactericidal (>3 log(10) CFU decrease within 24 h) against Staphylococcus aureus and S. epidermidis and bacteriostatic against Enterococcus faecalis and E. faecium. Fruticuline A manifested bacteriostatic activity against all tested strains. S. corrugata can be viewed as an interesting source for these diterpenes, which, if well tolerated in vivo, may represent new medical agents useful for the treatment of serious infections caused by resistant Gram-positive pathogens. 相似文献
127.
Roperto S Borzacchiello G Brun R Perillo A Russo V Urraro C Roperto F 《Veterinary pathology》2008,45(1):39-42
We report a case of multiple glomus tumors associated with bovine papillomavirus type 2 (BPV-2) infection in the urinary bladder of a 13-year-old cow suffering from severe chronic enzootic hematuria. Macroscopically, multiple submucosal reddish nodules were seen swelling the vesical mucosa. Histologically, neoplastic proliferation was characterized by the presence of numerous blood vessels. These were lined by normal endothelial cells surrounded by round epithelioid cells with central nuclei, prominent nucleoli, acidophilic cytoplasm, and well-defined cytoplasmic borders. Tumor cells were distributed around open vascular lumina and in perivascular spaces. They were immunohistochemically positive for actin and vimentin and negative for cytokeratins, desmin, and factor VIII-related antigen. On the basis of these findings, this tumor was diagnosed as glomus tumor, a neoplasm not previously reported in cattle and exceedingly rare in animals. BPV-2 DNA was amplified from the formalin-fixed, paraffin-processed tissue specimens obtained by laser capture microdissection. This report widens the spectrum of mesenchymal tumors of the bovine urinary bladder. Finally, the microscopic pattern of tumor described here shares striking morphologic and immunohistochemical similarities with the angiomatous form of glomus tumor known to occur in man. 相似文献
128.
129.
Zanetti S Bua A Molicotti P Delogu G Mura A Ortu S Sechi LA 《Acta veterinaria Hungarica》2008,56(2):145-152
During a six-month period a region of Northern Sardinia was monitored to check the presence of mycobacterial infections in wild boars. Forty-eight serum and 229 biopsy samples were collected from different animals and examined by both traditional diagnostic techniques (culture, bacterioscopic and molecular tests) and enzyme-linked immunosorbent assay (ELISA). The latter was used to determine the antibody response against both methylated and nonmethylated Heparin-Binding Haemagglutinin (HBHA) protein. Nine mycobacterial strains were isolated: three M. avium ssp. paratuberculosis (Map), three M. avium, one M. interjectum and two M. scrofulaceum strains. By PCR, only one animal was positive for M. bovis, whereas 10 animals were positive for Map. Out of the 48 sera tested, 19 showed a good humoral response to methylated HBHA and 17 to nonmethylated HBHA. Our data provide new information on the prevalence of mycobacterial infection among wild boars in Northern Sardinia and suggest that a more effective program should be developed to monitor mycobacterial infections in the wild animal population. 相似文献