基于ARM的育苗架智能监控系统设计

为了解决当前育苗过程中环境参数控制不便等问题,提出一种新的基于嵌入式ARM平台的育苗架智能监控系统。该系统搭载嵌入式Linux操作系统,采用Pt100温度传感器和NWSF-1AT湿度传感器,以及加热、加湿装置,可以对育苗架内的环境参数进行实时监测和智能控制。系统采用Modbus通信协议和RS-485数据接口,利用Qt开发用户界面,同时包含数据库功能,能够自动记录历史数据,便于后续的数据分析。大量实验表明,本系统可以实时监测育苗架内温湿度变化,当设定期望温度为25℃、湿度为40%RH时,能够有效地将育苗架内的温湿度环境稳定在设定值,能够为催芽育苗工作提供所良好的内部环境。     

日光温室青花菜耗水规律及水分利用效率的研究

在膜下滴灌条件下,研究了温室秋茬青花菜不同生育阶段控制水分对耗水规律、水分利用效率以及产量的影响。结果表明,青花菜全生育期内耗水量、耗水强度均表现为苗期﹥莲座期结球期的规律;水分生产函数的敏感指数为结球期﹥苗期﹥莲座期,因此青花菜节水宜在莲座期进行,结球期应保证水分供应充足,避免减产;苗期和莲座期分别控制灌水量为常规灌水量65%的处理,产量高、水分利用效率较高,是青花菜适宜的灌溉量。     

微分响应在降雨误差修正中的应用

误差修正是实时洪水预报研究和应用中的重要内容,从修正模型降雨输入角度出发,提出了一种基于微分响应的降雨误差修正方法,并通过实例论证了该方法修正降雨量的适用性。该方法通过计算降雨所对应模型的微分响应来修正降雨,从而修正出口断面流量过程。将该修正方法结合新安江模型,对闽江建阳流域的19场历史洪水进行了有效性检验,结果表明:此方法对洪量、洪峰的修正效果明显,且能够显著提高洪水预报的合格率,修正后合格率由63.2%提高到94.7%。     

电压空间矢量脉宽调制的简单算法

在电压空间矢量脉宽调制(SVPWM)控制技术原理的基础上,提出了一种简单快速算法,该算法只有普通的四则运算,计算变得非常简单,消除了常规的空间矢量控制算法中由于三角函数和无理数的近似计算而带来的计算误差及影响计算精度和速度的缺点,使结果更加准确。因而更适合应用于基于DSP 的数字化控制。理论分析和大量的MATLAB仿真结果表明,该算法具有谐波含量低、直流电压利用率高的优点。     

信息技术用于汽车纵梁制造的探讨

文章以为汽车行业的健康发展提供指导和借鉴为目的,主要对汽车的纵梁平面图纸集成化制造中诸如参数提取、纵梁3D模型构建技术和Agent的网络集成技术的应用方法等进行了相关论述,以此为实现汽车行业的可持续发展而做出贡献。     

基于"GIS/RS"技术的三江源地区生态环境建设的研究

分析了三江源地区生态环境退化现状，探讨了使用GIS与RS手段进行三江源区生态环境研究与建设的必要性与可行性，提出了研究方法与技术路线。     

Preparation and Identification of Canine Parvovirus NY Strain VP2 Protein Polyclonal Antibody

This study was aimed to prepare canine parvovirus (CPV) VP2 protein polyclonal antibody.The recombinant expression vector pET28a-CPV-VP2 was constructed and transfromed into E.coli BL21 (DE3),the expression of recombinant proteins was induced by IPTG from which the fusion protein was identified by SDS-PAGE.The target protein was purified and emulsify with adjuvant,the prepared immunogen was inoculated into rabbit by subcutaneous injections to prepare of VP2 protein specific polyclonal antibody.The immuno-activity,titers,neutralization titers of the prepared polyclonal antibody were determined by immunoperoxidase monolayer assay (IPMA).The results showed that the expressed recombinant protein VP2 (rVP2) existed in the form of inclusion body with a molecular weight of 72 ku.The prepared polyclonal antibody titer was 1 600 dilution,the virus titer was 10⁷ TCID₅₀/mL,the neutralizing titer was 1∶2 884.The antibodies showed specific reaction with CPV.In conclusion,rVP2 specific polyclonal antibody showed wonderful immunocompetence,specificity and neutralizing activity,providing foundation for the development of genetic vaccine and clinical therapeutic method.     

Effect of Weaning Stress on Blood Physiology and Biochemistry Indexes and Immune Function in Dairy Buffalo Calves

To study the effect of weaning stress on dairy buffalo calves,5 healthy calves were chosen and weaned,and the blood samples were collected at 1 day before weaning,and 1,14 and 21 days after weaning to analysis blood physiology and biochemistry indexes and immune function.The results showed that the count of white blood cell,lymphocyte,erythrocyte,monocytes and hematocrit,aspartate aminotransferase activity,blood glucose,cortisol and immunoglobulin G contents of dairy buffalo calves were no significant difference between before and after weaning treatments (P > 0.05).The count of neutrophils at 21 d after weaning were significantly lower than that of 1 d before weaning of dairy buffalo calves (P < 0.05).However,lactate dehydrogenase and alanine aminotransferase activity in weaned treatments were significantly higher than those of animals in the treatment before weaning (P < 0.05).The serum urea nitrogen of dairy buffalo calves at 21 d after weaning was significantly higher than that of 1 d before weaning (P < 0.05) and hemoglobin content at 21 d after weaning was significantly lower than that at 1 day before weaning (P < 0.05).It indicated that the physiological and immunity function of dairy buffalo calves were challenged under weaning stress.     

Study on Clinical Efficacy of Oligosaccharide Sulfate and Dextran Sulfate Against Bovine Mastitis

In order to detect the clinical efficacy of oligosaccharide sulfate(DEAE) and dextran sulfate (DS-4000) against bovine mastitis,56 cows with bovine mastitis were divided into 5 groups (DEAE group,DS-4000 group,DEAE＋penicillin group,DS-4000＋penicillin group and penicillin group),and their breasts were perfusion with different drugs twice one day for 3 days.The effective rates of drugs were observed by clinic symptoms.Milk samples were collected before and after treatment of drugs,and the bacterial negative conversion rate of each group were detected by microbial examination.The results showed that total effective rates of 5 groups were 10.00%,20.00%,58.33%,75.00% and 50.00%,respectively,and the bacterial negative conversion rates were 0,12.50%,50.00%,54.55% and 44.44%,respectively.In conclusion,the curative efficiencies of the polysaccharides against bovine mastitis were weak,but it could be improved by combination with penicillin.     

Effects of lncRNA-GTL2 Transgenosis on Gut Microbiota Composition in Mice

This research was aimed to study whether the lncRNA would have effects on the structure and constitution of the intestinal microbial colonies in mice.High throughout sequencing was used to sequence and analyze the intestinal microbial colonies in both transgenic and non-transgenic mice of three months old.We conducted comparison and t-test at the level of phylum and genus.The result showed that transferred into long non-coding RNA genes GTL2 (lncRNA-GTL2),the mouse intestinal microbial colony structure and composition had no significant differences in the overall,within the same gender,but there were individual differences.Therefore,this study didn't find that long non-coding RNA transfered had a significant impact on the intestinal microflorain the phylum and genus level.