Effect of Dietary Protein Concentration and Feeding Rate on Weight Gain,Feed Efficiency,and Body Composition of Pond-Raised Channel Catfish Ictalurus punctatus1

Two experiments were conducted in earthen ponds to evaluate the effect of dietary protein concentration and feeding rate on weight gain, feed efficiency, and body composition of channel catfish. In Experiment 1, two dietary protein concentrations (28% or 32%) and four feeding rates (≤ 90. ≤ 112, ≤ 135 kg/ha per d, or satiation) were used in a factorial arrangement. Channel catfish Ictalurus punctatus fingerlings (average size: 27 g/fish) were stocked into 0.04-ha ponds at a rate of 24,700 fish/ha. Fish were fed once daily at the predetermined maximum feeding rates for 282 d (two growing seasons). In Experiment 2, three dietary protein concentrations (24, 28, or 32%) and two feeding rates (≤ 135 kg/ha per d or satiation) were used. Channel catfish (average size: 373 g/fish) were stocked into 0.04-ha ponds at a rate of 17,300 fish/ha. Fish were fed once daily for 155 d. In both experiments, five ponds were used for each dietary treatment. Results from Experiment 1 showed no differences in total feed fed, feed consumption per fish, weight gain, feed conversion ratio (FCR), or survival between fish fed diets containing 28% and 32% protein diets. As maximum feeding rate increased, total feed fed, feed consumption per fish, and weight gain increased. There were no differences in total feed fed, feed consumption per fish, or weight gain between fish fed at ≤ 135 kg/ha per d and those fed to satiation. Fish fed the 28% protein diet had a lower percentage carcass dressout and higher percentage visceral fat than fish fed the 32% protein diet. Dietary protein concentrations of 28% or 32% had no effect on fillet protein, fat, moisture, and ash. Feeding rate did not affect FCR, survival, percentage carcass dressout, or fillet composition, except fillet fat. As feeding rate increased, percentage visceral fat increased. Fish fed at ≤ 90 kg/ha per d had a lower percentage fillet fat than fish fed at higher feeding rates. In Experiment 2, dietary protein concentration or maximum feeding rate did not affect total feed fed, feed consumption per fish, weight gain, FCR, or survival of channel catfish. Feeding rate had no effect on percentage carcass dressout and visceral fat, or fillet composition. This was due to the similar feed consumption by the fish fed at the two feeding rates. Fish fed the 24% protein diet had lower carcass dressout, higher visceral fat and fillet fat than those fed the 28% or 32% protein diet. Results from the present study indicate that both 28% and 32% protein diets provide satisfactory fish production, dressed yield, and body composition characteristics for pond-raised channel catfish fed a maximum rate of 90 kg/ha per d or ahove.     

大功率磁力驱动泵的研制

介绍了大功率磁力驱动泵的原理，结构设计、主要零件的材质选择及加工难点，并由此制作出性能令人满意的样机。     

用突变模型研究森林蓄积的稳定性(英文)

模型构造的方法是以Logistic方程描述生长量 ,假设采伐量为常量且等于生长量时 ,推导出折迭突变模型 .此模型有助于管理层制定管理决策 ,防止森林消亡 .该文又通过曲线采伐模型及Logistic生长模型推导出尖角突变模型 ,这个模型给出了采伐将导致突变发生的敏感区域 ,而且通过模型的滞后性使人们清楚地意识到森林一旦遭受破坏 ,再想恢复需入更大的力量     

冬小麦越冬期茎尖超微结构变化与玉米赤霉烯酮的关系

应用酶联免疫技术和电镜技术，研究了冬小麦品种燕大１８１７越冬期内源玉米赤霉烯酮（ＺＥＮ）含量和茎尖超微结构的变化，结果表明，随着秋末冬初气温逐渐降低，日照缩短，茎尖ＺＥＮ含量逐渐增加，１１月下旬含量达到高峰，随后急剧下降，到１月下旬又出现一个小的含量高峰。在小麦茎尖ＺＥＮ含量出现高峰前后（１１月底，１２月初），茎尖细胞中线粒体和质体的体积增大，形状也发生变化，线粒体从低温诱导前的圆球形变为长形、哑     

In vitro differentiation of exocrine pancreatic cells from mouse embryonic stem cells

AIM: To explore the effects of sodium butyrate, activin A and dexamthasone on inducing mouse embryonic stem (ES) cells to differentiate into exocrine pancreatic cells in vitro. METHODS: E14 mouse ES cells were cultured in suspension to form embryonic bodies (EBs). The EBs were cultured with differentiating medium containing different concentrations of sodium butyrate, and the spontaneously differentiated ES cells were used as control. Exocrine pancreatic genes such as amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected by RT-PCR at different time points to determine the optimal concentration and exposure time of sodium butyrate. Furthermore, activin A or dexamthasone was also used to explore the effects on exocrine differentiation. After that, the combination of sodium butyrate, activin A and dexamthasone was used to promote the differentiation of exocrine pancreatic cells from ES cells. During the differentiation course, the gene expressions of amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected by RT-PCR. Morphological changes were investigated by phase contrast microscopy. Amylase expression was examined by immunofluorescence staining. RESULTS: Exocrine pancreatic gene expressions such as amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected in spontaneously differentiated EBs. A relatively lower concentration of sodium butyrate with a shorter exposure time significantly promoted those above gene expressions as compared to that of spontaneously differentiated EBs. Activin A and dexamethasone induced upregulation of exocrine gene expression. The combination of activin A, sodium butyrate and dexamethasone significantly enhanced the mRNA levels of amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase. Under the treatment of activin A, sodium butyrate and dexamethasone, differentiated cells were polygonal in shape with large, round, and center-situated nuclei. According to the observation of immunofluorescence staining, amylase was positive expressed at the final stage. CONCLUSION: These data indicate that exocrine pancreatic differentiation of ES cells is induced by sodium butyrate, activin A and dexamethasone. The combination of pancreatic inducing factors improves the differentiating efficiency.     

云南省大黄藤资源培育的研究

大黄藤具有较高的药用价值.在介绍大黄藤的适生环境及药用价值的基础上,论述了云南省的大黄藤资源状况及人工种植情况,针对其开发利用价值优势,对云南大黄藤的资源培育提出了5点建议.     

一种简易加工气调袋装置的开发

目前,国内已有专门生产的硅窗气调袋的机械设备,但生产的品种与规格均有限,难以满足品种繁多的果蔬贮藏要求。由于需求的批量小,使气调袋生产成本增加,限制了其推广应用。为此,开发了一种用于加工硅窗气调袋的简易热合装置,该装置投资成本低、操作简单,适合小批量生产加工,并可以根据要求生产出不同硅窗面积的气调袋,具有现有加工机械不可替代的优势。     

多节小麦遗传资源的发现

普通小麦地上部份茎朴伸长节为5～6节，是一个相当稳定的遗传特性，普遍存在于小麦属以至小麦族内的一年生种中。在Ф．Х.бахТееВ（1971）根据Nilan等的研究结果编制的大麦已定位基因一览表中，在第2和第3染色体上各有一对“多节矮生”基因（m1，m2），并另有一对未定位的多节矮生基因（m）。中国浙江省农业科学院大麦室在20世纪70年代初，以^60Coγ射线辐射处理早熟3号大麦品种，选择到一个“特矮多节”的二棱大麦突变体。但迄今国内外尚未见在普通小麦中发现多节突变体的报道。     

关于改进和完善野生动植物进出口管理有关问题的思考

野生动植物及其产品的进出口管理是野生动植物管理工作的重要组成部分.近半个世纪尤其是改革开放二十多年来,我国的野生动植物国际贸易发展迅速,贸易物种和产品增多、贸易形式多样、频度增大.我国在野生动植物贸易管理立法与执法、行政与技术措施、发展产业推动经济文化发展、创造国际环境等方面都取得了重要进展.随着野生动植物保护问题日益国际化、我国市场经济的发展尤其是加入WTO,我国野生动植物贸易管理面临许多新问题、新挑战.改进和完善我国野生动植物进出口管理和履约工作,必须要处理好对外关系,抓紧改进审批管理,完善贸易许可证管理制度,加强监督和制约机制的建立和完善,不断改进工作作风,加强管理队伍和执法队伍建设.     

Studies of Multi-Allelic Polymorphism of Dominant Dwarfing Genes in Wheat

Dwarfing breeding of wheat in the world is confined to the exploitation of recessive dwarfing sources. None of the dominant dwarfing sources discovered in common wheat (Triticum aestivum L. ) has found wide exploitation in wheat breeding due to the extreme dwarfness of their plants (20 -55 cm). We found in our work that some stable mutant lines with their plant height enhanced to different extents could be obtained in large populations derived from the stock seeds of the dominant dwarfing sources Aibian1 carrying Rht10 on 4DS and being 20 - 55 cm tall and Aisu2 carrying Rht3 on 4BS and being 55 cm tall, or from their descendants of induced mutation treatments, or from the segregating descendants of their crosses with mid- or tall-statured genotypes. Subsequently, we studied these mutation-derived lines differing in plant height with near isogenic lines and observed that the character of their enhanced plant height bred true, each carrying a semidominant dwarfing gene for a definite height and that as the plant height of the mutation-derived lines increased, the yield-contributing characters of their near isogenic lines were significantly improved. When test crosses with marker genes and physiological and biochemical genetic marker tests were performed to re-localize the semi-dominant dwarfing genes carried by the mutation-derived lines, it was confirmed that they shared common loci with Rht10 and Rht3 and that they were all mutation-derived multiple alleles. It is thus speculated that dominant dwarfing genes are of "multi-allelic polymorphism". In other words, dominant dwarfing genes, which are ultra-dwarfing, are liable to develop by mutation into a group of multiple alleles with plant height enhanced to different extents and some may have a height close to the ideal plant height for wheat breeding. Therefore, these results offer a fundamentally new approach for the exploitation of dominant dwarfing sources in wheat breeding.