生活垃圾微生物强化堆肥对放线菌群落的影响

为了探讨微生物强化堆肥对生活垃圾好氧堆肥过程及堆肥过程中放线菌群落的影响,在堆肥过程中接种高效细菌复合菌剂和真菌复合菌剂,并以不接种的堆体为对照,对堆肥过程的温度变化和木质纤维素的降解效率进行了测定,并借助于PCR-DGGE方法对堆肥过程中放线菌群落的动态变化和种群多样性进行研究。结果表明：微生物强化堆肥能缩短堆体起爆时间,并能有效提高堆体降温期和二次发酵期的温度;和自然堆肥相比微生物强化堆肥使半纤维素、纤维素和木质素的降解率分别提高8.95%、12.72%和10.13%。DGGE图谱显示：2种堆肥方式的放线菌多样性指数表现出极显著差异,微生物强化堆肥能增加堆体中优势菌群的种类和数量,能有效提高腐熟期的放线菌群落多样性,有利于堆肥腐熟。优势条带测序结果表明：在接种堆肥过程中检测到了放线菌门的棒杆菌属、分支杆菌属、链霉菌属、热孢菌属、迪茨菌属、糖丝菌属和放线菌属。     

棉花叶螨的发生及其综合防治技术措施

该文阐述了棉叶螨形态、危害症状、发生规律、传播途径和因地制宜地采取农业、化学、生物等多种手段进行综合防治技术措施。     

Effect of biofertilizers on the nutrient availability in soil in relation to growth,yield and yield attributes of Stevia rebaudiana

A field experiment was conducted at Rani Chandramma Agricultural University (Arabhavi, Belgaum), Karnataka, India, in basic soil to study the nutrient availability in soil, yield and yield attributes of the medicinal plant stevia (Stevia rebaudiana). The results showed that the availability of nutrients such as nitrogen (N), phosphorus (P) and potassium (K) in the soil increased up to the fifth month; and thereafter, decreased by the same amount with the progress of the plant growth up to the sixth month, irrespective of treatments. Results also showed that the fresh and dry biomass yields significantly increased up to the sixth month with different treatments, but the yield attributes like plant height and the number of branches were observed to be varied with various treatments, being highest in the combined application of biofertilizers over that of their corresponding sole applications.     

Quantitative methods for estimating foliar uptake of zinc from suspension‐based Zn chemicals

The present study developed methods for quantifying foliar Zn uptake from suspension‐based Zn chemicals of low solubility, which were ZnO (particle size: 0.15^–1.34 μm) and a newly synthesized Zn hydroxide nitrate crystal (Zn‐HNC) (50^–100 nm thickness and 0.2^–1 μm lateral dimension). Recently matured leaves of citrus (Citrus aurantium L. cv. Valencia), capsicum (Capsicum annume L. cv. Giant Bell), and/or tomato (Solanum lycopersicum L. cv. Roma) were in vitro–treated with microdroplets (5 μL per droplet) of Zn‐HNC‐ and ZnO‐suspension solutions on the adaxial surface and incubated under controlled conditions for up to 72 h. Leaf‐washing protocols were compared, including: dilute ethanol (3%), dilute nitric acid (2%), and their combination. The methods for quantifying Zn uptake were: (1) whole‐leaf loading by which droplets of the Zn suspension solutions were loaded onto central regions of both left and right sides of leaf blades and (2) half‐leaf loading by which soluble‐Zn (ZnSO₄) droplets were loaded onto only one side of leaf blades while the other was used as the background Zn control. Foliar‐surface characteristics of the plant species affected the effectiveness of the washing methods. The dilute nitric acid (2%; ± 3% ethanol) was required to remove residue particles of the ZnO and Zn‐HNC suspensions from foliar surfaces of capsicum and tomato (highly trichomatic), but the residue Zn chemicals on citrus leaves (nontrichomatic and highly waxy) were similarly and effectively removed by the three washing methods. For quantifying Zn uptake by the leaves, the half‐leaf loading method showed its advantages over the whole‐leaf loading method, because it did not stringently require similar background Zn concentrations in the control and treated leaves at the start and had little risks of secondary absorption of soluble Zn in the washing solution.     

RNA干扰对烟草NtPPO8基因沉默的效应分析

多酚氧化酶（PPO）是发生酶促褐变的关键酶,PPO活性过高会使烟叶变褐。为明确烟草NtPPO8基因对PPO活性的影响,建立降低NtPPO8基因表达量的病毒诱导的基因沉默（virus induced gene silencing,VIGS）体系,探究使用RNA干扰技术对烟叶NtPPO8基因表达量及PPO活性的影响。以中烟100和K326为材料,分析在移栽后50d进行VIGS载体侵染的植株,并在侵染后10、20、30、40d分别测定NtPPO8基因表达量及PPO活性。结果表明,接种VIGS载体的转化植株,其中在侵染后20d时NtPPO8基因的沉默效率最高,为78.46%;随着侵染时间推移,NtPPO8基因的沉默效率逐渐降低,在接种后40d内有效表达;且转化植株中的病毒载体会随着植株的生长而向上传导,随着接种部位的上移,沉默效率逐渐降低。因此,研究建立VIGS体系能够有效沉默NtPPO8基因的表达,并且有效降低PPO活性,为提高烟叶耐烤性及降低烟叶褐变比例提供了新的思路和方法。     

采煤塌陷裂缝对降雨后坡面土壤水分的影响

为探究采煤塌陷裂缝对坡面储蓄降水的影响,本文对降雨后不同坡向上裂缝两侧的不同土层水分动态变化特征进行了研究。结果表明:降雨主要补充坡面地表0~20 cm土层土壤水分,且在雨后0~20 cm土层土壤水分散失也较为严重;雨后阴坡土壤含水率最高,0~10cm、10~20cm和20~40 cm土层土壤水分差异显著(p0.05)。降雨结束8 d后,裂缝附近的土壤水分变化幅度较大,尤以坡面裂缝上部边缘处最为明显,裂缝处与远离裂缝的土壤水分之间差异显著(p0.05);阳坡土壤水分损失最高,阳坡裂缝周边土壤水分平均损失量高达3.31%。可见,裂缝的出现会在一定程度上打破坡面储蓄降水的格局,加剧坡面局部土壤水分散失,这一点在植被恢复与建设过程中不容忽视。     

Relationship between photosynthesis and leaf nitrogen concentration in ambient and elevated [CO2] in white birch seedlings

To study the effects of elevated CO2 concentration ([CO2]) on relationships between nitrogen (N) nutrition and foliar gas exchange parameters, white birch (Betula papyrifera Marsh.) seedlings were exposed to one of five N-supply regimes (10, 80, 150, 220, 290 mg N l(-1)) in either ambient [CO2] (360 micromol mol(-1)) or elevated [CO2] (720 micromol mol(-1)) in environment-controlled greenhouses. Foliar gas exchange and chlorophyll fluorescence were measured after 60 and 80 days of treatment. Photosynthesis showed a substantial down-regulation (up to 57%) in response to elevated [CO2] and the magnitude of the down-regulation generally decreased exponentially with increasing leaf N concentration. When measured at the growth [CO2], elevated [CO2] increased the overall rate of photosynthesis (P(n)) and instantaneous water-use efficiency (IWUE) by up to 69 and 236%, respectively, but decreased transpiration (E) and stomatal conductance (g(s)) in all N treatments. However, the degree of stimulation of photosynthesis by elevated [CO2] decreased as photosynthetic down-regulation increased from 60 days to 80 days of treatment. Elevated [CO2] significantly increased total photosynthetic electron transport in all N treatments at 60 days of treatment, but the effect was insignificant after 80 days of treatment. Both P(n) and IWUE generally increased with increasing leaf N concentration except at very high leaf N concentrations, where both P(n) and IWUE declined. The relationships of P(n) and IWUE with leaf N concentration were modeled with both a linear regression and a second-order polynomial function. Elevated [CO2] significantly and substantially increased the slope of the linear regression for IWUE, but had no significant effect on the slope for P(n). The optimal leaf N concentration for P(n) and IWUE derived from the polynomial function did not differ between the CO2 treatments when leaf N was expressed on a leaf area basis. However, the mass-based optimal leaf N concentration for P(n) was much lower in seedlings in elevated [CO2] than in ambient [CO2] (31.88 versus 37.00 mg g(-1)). Elevated [CO2] generally decreased mass-based leaf N concentration but had no significant effect on area-based leaf N concentration; however, maximum N concentration per unit leaf area was greater in elevated [CO2] than in ambient [CO2] (1.913 versus 1.547 g N m(-2)).     

A soil temperature control system for ecological research in greenhouses

We designed and tested a soil temperature control system for plant ecophysiological experiments in greenhouses and growth chambers. The system consists of a plywood box, polyethylene liner, insulation, seedling containers, a water pump, and a flow-through heater or chiller. One hundred and twelve seedling containers (11cm diameter, 13.5 cm high) are mounted in the plywood box. There is a hole at the bottom center of each container to allow the free drainage of irrigation water and fertilizer solution. The space between containers is filled with water that is circulated through the chiller/heater. The water is also circulated within the plywood box by a water pump to increase the uniformity of temperature. The system was tested for three soil temperatures (5, 20, and 30°C) over a period of four months. The containers were filled with a peat-moss vermiculite mixture and planted with tree seedlings. The test showed that the soil temperature was almost equal to the water temperature for all three soil temperatures (regression slop = 0.99, intercept = 0.12,r ² = 1.00). The average soil temperatures were within (0.41°C of the set values. The soil temperature of the 112 containers within the same box followed a normal distribution with a small standard deviation (0.34°C for the 30°C treatment). There was a temperature gradient from the top to the bottom of the container (< 1°C). The direction of the temperature gradient was determined by the direction of temperature difference between the soil and the ambient air. When the soil temperature was lower than air temperature, the soil temperature decreased from the top to the bottom of the container, and vise versa. The soil temperature was higher during the day than at night (difference < 1.5°C). Funding for this research was provided by Lakehead University, CFI, ORDCF, and NSERC research grants to Dang, and an NSERC PGS A to Cheng.     

咸水沟灌对土壤水盐变化与棉花生长及产量的影响

为持续高效利用咸水资源,在棉花长期定位咸水沟灌试验(始于2006年)的基础上,研究了不同矿化度(1、2、4、6、8、10 g/L)咸水连续灌溉第10年土壤水盐分布与棉花生长响应以及历年土壤盐分和籽棉产量的变化特征。结果表明:1)年际间,各处理0～100 cm土层土壤盐分受灌溉和降水影响而波动,但未随灌溉年限的增加而逐渐累积,灌溉水矿化度≤4 g/L处理可基本维持土壤盐分周年补排平衡。单个棉花生长季(2015年),各处理沟底部位的土壤含水率大于垄上,灌溉水矿化度≥6 g/L时主根层土壤含水率高于1 g/L处理;土壤盐分随灌溉水矿化度增加而增大,随棉花生育期的推进先增大后降低,灌水沟剖面土壤盐分呈向垄上和深层运移的趋势;与播种时比,棉花收获后各处理主根层土壤盐分均未出现累积。2)低矿化度咸水沟灌对棉花成苗率、株高和叶面积指数影响不明显,超过一定限值后3项指标显著下降,与1 g/L处理相比,当灌溉水矿化度达到6 g/L时棉花成苗率和叶面积指数显著降低,当灌溉水矿化度达到8 g/L时株高显著降低;咸水沟灌对棉花纤维品质影响较小,5项品质指标在处理间差异均不显著。3)适量浓度的咸水灌溉对籽棉产量影响较小,与1 g/L灌水处理相比,2和4 g/L处理对历年籽棉产量(2006-2015年)无显著影响,大于6 g/L时历年籽棉产量显著降低。在该研究灌溉制度下,推荐试验区咸水沟灌棉花的灌溉水矿化度阈值为4 g/L。