Research on biological invasions has traditionally focused on the ecological component of invasive alien species, either without considering or by considering in a restricted way, the knowledge of the social component. An understanding of the human dimension of invasions is critical to effectively tackle the problems associated with invasive alien species. We distributed questionnaires to evaluate the social perceptions and attitudes of different stakeholder groups affected by invasive alien species in the Doñana social-ecological system (SW Spain). Our analysis served to characterize respondents based on their knowledge and perceptions of the impacts of invasive alien species, and the attitudes toward their introduction and eradication. Hierarchical cluster and principal component analyses identified five categories of respondents, and potential support of eradication programs was assessed using a contingent valuation approach. The five stakeholder groups differed in their degree of knowledge, perceptions, attitudes, and willingness to pay for eradication. The fact that different stakeholders have remarkably different perceptions about the impacts and benefits caused by invasive alien species, and different attitudes toward their introduction or eradication should be considered in any decision-making process regarding their management, particularly when developing appropriate educational and informative programs. Consultation with different stakeholders should also be encouraged from the onset of any decision-making process. In this way, trade-offs involved in IAS management are directly addressed and successful implementation of management practices is facilitated. 相似文献
In water systems, water quality and geochemical properties of sediments determine the speciation of trace metals, metal transport, and sediment–water exchange, influencing metal availability and its potential effects on biota. Studies from temperate climates have shown that iron-ore mining and tailing wastewaters, besides being a source of trace metals, usually show high levels of dissolved ions and particulate suspended matter, thus having the potential of indirectly changing metal bioavailability. For the first time in the tropics, we identified the effects of iron-ore mining and processing on metal bioavailability in a coastal lagoon. With an extensive sampling scheme, we investigated the potential sources of metals; the links among metal levels in water, sediments, and invertebrates; and the contrasting effects on metal speciation and bioavailability.
Methodology
The metals Fe, Mn, Al, Cr, Zn, Cu, Ni, Pb, Cd, Hg, and As were measured in water, sediments (surface and profiles), and invertebrates from Mãe-Bá Lagoon and in the sites directly influenced by the mining operations (tailing dams and nearby rivers). In addition, samples from two other lagoons, considered pristine, were analyzed. The study area is located in the southeast of Brazil (Iron Quadrangle Region and a coastal area of Espírito Santo State). General water characteristics included pH, dissolved organic carbon, alkalinity, and anion composition. Water metal speciation was assessed by a speciation model (Chemical Equilibria in Aquatic Systems). Grain-size distribution, organic carbon, carbonate, and acid volatile sulfide (AVS) were determined in sediments. Statistical methods included comparison of means by Mann–Whitney test, ordination and correlation analyses, and analysis of regression for geochemical normalization of metals with grain size.
Results and discussion
The dissolved metal concentrations, the total metal levels in sediments, and the normalization based on the fine sediment fraction showed that the mining operations constitute potential sources of Fe, Mn, Cr, Cu, Ni, Pb, As, and Hg to Mãe-Bá Lagoon. However, trace metal availability was reduced because of increased pH, hardness, and sulfide content (356 μmol/g) in the sites influenced by the mining. The lagoon showed similar water chemistry as in the mining sites, with metal bioavailability further decreased by the presence of dissolved organic carbon and chloride. Although AVS levels in the lagoon were low (0.48–56 μmol/g), metal bioavailability was reduced because of the presence of organic matter. Metal levels in invertebrates confirmed the predicted low metal bioavailability in Mãe-Bá Lagoon. The lagoon was considered moderately contaminated only by Hg and As.
Conclusions
The iron-ore mining and processing studied here constitute potential sources of metal pollution into the tropical lagoon. Contrary to expectations, however, it also contributes to reducing the overall metal bioavailability in the lagoon.
Recommendations and perspectives
These findings are believed to be useful for evaluating metal exposure in a more integrated way, identifying not only the sources of pollution but also how they can affect the components involved in metal speciation and bioavailability in water systems, leading to new insights. 相似文献
Vitamin A deficiency (VAD) is a significant public health problem in many countries. While cost-effective interventions are available to control VAD, reliable information is needed to the track progress of control programmes. However, assessment of VAD is uncommon because current approaches are expensive and not feasible in low-resource settings. The present study explores the utility of retinol-binding protein (RBP), analysed by enzyme-linked immunosorbent assay from capillary blood, as an alternative measure of serum retinol concentrations in populations. The study collected matched panels of venous and capillary blood from pre-school children in Chiang Mai, Thailand. Of a total sample of 195 children, there were no differences between RBP from venous blood, RBP from capillary blood or retinol from capillary blood relative to retinol from venous blood. Receiver-operating characteristic curve analysis suggested a cut-off of RBP < 0.825 micromol l(-1) had optimal screening proficiency relative to retinol <0.70 micromol l(-1). For the purpose of population assessment, all three parameters performed well in screening for VAD relative to retinol from venous blood. There were no differences in the estimates of VAD between children stratified by inflammation status. Lower RBP concentrations were found in children in the early convalescent stage of infection than in children with no infection or in the late convalescent stage. This study provided evidence of the biological comparability between retinol and RBP estimated from venous blood and capillary blood. This is a critical observation as it provides empirical evidence that RBP from capillary blood is a surrogate measure of serum retinol concentrations. 相似文献
Solanum okadae Hawkes et Hjert is a wild diploid potato species endemic to Argentina and Bolivia, of potential breeding value. However,
no genetic studies have been carried out with this species and its crossability relations with the common tetraploid potato,
S. tuberosum L. ssp. tuberosum, are unknown. Furthermore, accessions from both countries differed in their morphological phenotypes. To ascertain the feasibility
of incorporating this wild germplasm into cultivated potato, 2n pollen screening was carried out in 10 accessions and families
derived from crosses between accessions; also, pollen-pistil compatibility relations were studied in reciprocal interspecific
crosses. Plants of four of the 10 accessions produced 2n pollen (0.1 to 5.0%) and 4n pollen (0.0 to 3.0%). Parallel and tripolar
spindles at Anaphase II were the cytological mechanisms involved in 2n pollen formation; lack of chromosome migration in both
meiotic divisions originated the 4n pollen. Both full compatibility and incompatibility at various sites along the pistil
were observed in the S. tuberosum × S. okadae combinations; most reciprocal combinations were incompatible. Compatible genotypes produced 2n pollen. However, only a few
seeds were obtained and chromosome counts could not be carried out in the hybrid progeny because seedlings died at an early
stage. The pollen-pistil barriers are incomplete and can be circumvented by the appropiate choice of parents. The identification
of the post-zygotic barriers will be the focus of further studies. 相似文献
Loquat species (Eriobotrya japonica Lindl.) originated in China, later was introduced in Japan. However, introduction in Europe occurred much later, in 18th
century, it was introduced as an ornamental tree. Since then, the species was very well adapted to the Mediterranean climate,
mainly in South East of Spain, where is located more than 50% of total European crop production. A European Project titled
‘Conservation, evaluation and collection of underutilized fruit species’ supported surveys and collection of plant material
from these species in the Mediterranean basin countries. One of these surveys resulted in a germplasm collection of loquat
established at IVIA, Valencia, Spain. This collection is being extended with new accessions collected across the world, currently
the collection have 123 accessions under study. Germplasm management needs first a characterization of the plant material
introduced and second diversity studies into the collection that could point out the variability pattern, to establish groups
of accessions with similar traits and the most significant variables. These results allow organizing the information gathered
for further uses. In this paper, 62 accessions from the IVIA germplasm collection were studied. Results from 43 variables
were gathered and data studied by multivariate analysis. The analysis allowed summarizing the data tables with new variables
containing most of the information. These results along with those obtained in the coming years will allow define a nuclear
collection (minimum number of accessions that contained all the variability present in the collection). 相似文献
Tejocote (Crataegus spp.) is a genus of fruit-bearing trees distributed widely throughout Mexico; 13 species are reported for the north and central
zones and two or more species may be present in southern Mexico. Accessions of this genus are safeguarded in the Germplasm
Bank of Tejocote at the Autonomous University of Chapingo, mainly from three regional sources, i.e. the states of Puebla,
Mexico, and Chiapas, including five different species that belong to series Mexicanae and series Crus-galli. They can be morphologically
characterized by leaves from different shoot types, flowers and fruits. Multivariate statistical methods were used to elucidate
patterns of variation in each of these regional sources. The sources displayed very low intra-source variability. The source
from Chiapas showed significant statistical differences in all morphological variables evaluated, as a result, this genetic
pool is considered as different from the other two sources. The sources from the states of Puebla and Mexico only differed
by 22.79% (with P ≤ 0.05), and thus they could be considered as components of a single genetic pool. The most highly discriminant variables
were from the leaf, such as basal angle, petiole length/major axis length ratio, minor axis length/major axis length ratio,
and number of veins.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
Pinus patula is a species commonly used for reforestation in Mexico.However,efficient methods for the mass production seedlings are required.Micropropagation particularly by somatic embryogenesis provides an option for the rapid multiplication of high-quality,genetically improved material.This study induces somatic embryogenesis in this species using the thin cell layer(TCL)technique.Two sources of explants(complete immature embryos;lTCL segments from immature embryos)were evaluated.The efficiency of TCL from longitudinal sections[lTCL]and transverse[tTCL]was evaluated.The results show using thin cell layers from immature embryos cultivated in 16 light/8 dark hours achieves induction of somatic embryos.A higher percentage of embryogenic callus was obtained when tTCL segments were used as an explant source.These results produced somatic embryos from tTCL segments of an immature embryo without germinating the seed,making the process more time efficient.In addition,this technique can be used to generate somatic embryogenesis in forest species that have low germination rates. 相似文献
Several interactions between Al and the solid phase of soil influence Al buffering in soil solution. This work evaluated soils cultivated with Pinus taeda L. to determine Al forms in organic and mineral horizons using various extraction methods and to relate acidity with clay mineralogy.
Materials and methods
Organic and mineral horizons of 10 soil profiles (up to 2.1 m deep) in southern Brazil were sampled. Organic horizons were separated into fresh, aged, and fermented/humified litter. The following Al extraction methods were utilized: 0.5 mol L?1 pH 2.8 CuCl2–Al complexed in organic matter; 1.0 mol L?1 KCl–exchangeable Al; water–Al soluble in soil solution; HF concentrated?+?HNO3 concentrated?+?H2O2 30% (v/v)–total Al. Six sequential extractions were carried out to isolate different forms of amorphous minerals that can buffer Al on soil solution: 0.05 and 0.1 mol L?1 sodium pyrophosphate; 0.1 and 0.2 mol L?1 ammonium oxalate; 0.25 and 0.5 mol L?1 NaOH. Samples of clay were also analyzed by XRD.
Results and discussion
There was a clear effect of litter age on increasing total Al concentration. In the aged litter and fermented and/or humified litter, levels of total Al were 1.4 to 3.8 and 1.5 to 7.8 times greater than in fresh litter, respectively. The CuCl2 method had higher Al extraction capacity than the KCl method for litter. The lowest Al–pyrophosphate values were observed in the Oxisol, which also had a predominance of gibbsite and the lowest levels of Al–KCl and Al–CuCl2. There was an inverse relationship between degree of soil weathering and soluble and exchangeable Al in soils. Available Al increased with higher Si proportion in minerals of the clay fraction (2:1?>?1:1?>?0:1).
Conclusions
The worst scenario was soils with the combination of high soluble and exchangeable Al levels and high concentrations of amorphous forms of Al minerals. The best predictors of Al accumulation in the youngest litter horizon were extractions of amorphous minerals with pyrophosphate and NaOH. These extractors are normally used to predict the level of Al buffering in soils. Organic matter had less influence on Al dynamics in soils.
If we are to breed common bean (Phaseolus vulgaris L.) for durable resistance to diseases, we must understand pathogenic variation
and find sources of resistance. Our first objective was to determine the patterns of pathogenic variation found among isolates
of Phaeoisariopsis griseola (PG), the fungus that causes angular leaf spot (ALS) in common bean. We characterized 433 PG isolates
from 11 Latin American and 10 African countries, using differential cultivars, isozymes, and/or random amplified polymorphic
DNA (RAPD) markers. We also systematically screened, for ALS resistance, common bean accessions from the world collection
held at CIAT, and assessed the progress so far made in breeding for resistance to ALS. Despite their great diversity within
and between countries on both continents, the PG isolates were classified into two major groups: Andean, and Middle American.
Although each group had internal differences for virulence, and biochemical and molecular characteristics, the ‘Andean’ PG
isolates were more virulent on common beans of Andean origin, than on those of Middle American origin, thus, suggesting a
host-pathogen co-evolution. The ‘Middle American’ PG isolates, although more virulent on common beans from Middle America,
also attacked Andean beans, thus, exhibiting a much broader virulence spectrum. To find sources of resistance, we tested 22,832
common bean accessions against naturally occurring PG isolates in the field at CIAT's Experiment Station, Quilichao, Colombia,
between 1985 and 1992. The resulting 123 intermediate (scores of 4 to 6) and resistant (scores of 1 to 3) accessions were
then tested in the greenhouse against selected 14 PG isolates of diverse origins. Nineteen accessions were intermediate or
resistant to at least 13 of 14 PG isolates. Similarly, of 13,219 bred lines tested in the field between 1978 and 1996, 89
were intermediate or resistant. Of these, 33 bred lines proved intermediate or resistant to at least eight of nine PG isolates
to which they were challenged in the greenhouse. We suggest that, to breed for durable resistance to ALS, common bean populations
should be developed from crosses between Andean and Middle American gene pools. The populations should then be systematically
evaluated and selected against the broadest range of the most virulent PG isolates of diverse evolutionary origins.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
This study investigates the plastic responses after shoot removal of Amaranthus cruentus L. and A. hypochondriacus L. This practice is common in the Sierra Norte de Puebla, Mexico, where both species are cultivated and used as a food source during the vegetative stage. Empirical observations indicate that biomass increases in clipped plants. The experimental study carried out on both species of Amaranthus imitated local farm management. Three levels of shoot removal were applied to plants of both species: Control, 10% and 40%. In A. cruentus plants with 10% of the shoot removed produced more stalk biomass, more leaves, greater leaf area, greater length of primary branches and a larger number of secondary branches than the control plants. In A. hypochondriacus, there were no differences among levels of shoot removal in stem biomass; in general, the number of primary and secondary branches in control plants was greater than that in clipped plants. Patterns of biomass allocation revealed important differences between species, as clipped plants allocated more biomass to seeds in A. cruentus, while control plants allocated more to leaves in A. hypochondriacus. Furthermore, changes in response at the primary branch (secondary branches, leaf biomass, and area) level were observed between species. In general, clipped plants of A. cruentus overcompensate for the damage, while this response was not evident in clipped plants of A. hypochondriacus. Instead, this species showed high tolerance to damage. The phenotypically plastic responses expressed for both species can be used as a guide to improve management of these plants by local farmers from the Sierra. 相似文献