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101.
The nuclear genomes of multicellular animals and plants contain large amounts of noncoding DNA, the disadvantages of which can be too weak to be effectively countered by selection in lineages with reduced effective population sizes. In contrast, the organelle genomes of these two lineages evolved to opposite ends of the spectrum of genomic complexity, despite similar effective population sizes. This pattern and other puzzling aspects of organelle evolution appear to be consequences of differences in organelle mutation rates. These observations provide support for the hypothesis that the fundamental features of genome evolution are largely defined by the relative power of two nonadaptive forces: random genetic drift and mutation pressure. 相似文献
102.
Mary Jo Burkhard Sarah Leavell Rachael B. Weiss Kody Kuehnl Hope Valentine G. Thomas Watters Barbara A. Wolfe 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(4):426-436
Background: Freshwater mussels are among the most endangered taxa in North America and minimally invasive techniques to evaluate their health are needed. Objective: The objective of this study was to develop a standardized approach for identifying and enumerating the cellular components of freshwater mussel hemolymph. Methods: Hemocyte clumping, total hemocyte count, and hemocyte morphology were compared in untreated hemolymph or hemolymph treated with formalin, sodium citrate, sodium heparin, EDTA, water, or l ‐cysteine. Morphology was then used to categorize hemocytes and perform a 100‐cell differential. Results: Treatment with formalin or >25 mg/mL l ‐cysteine reduced hemocyte clumping, although only formalin significantly increased the total hemocyte count. However, formalin also induced crenation that impaired hemocyte identification. Both EDTA and sodium citrate‐induced hemocyte degranulation while sodium citrate and >40 mg/mL l ‐cysteine‐induced cell lysis. Hemocytes could be categorized into 2 groups of granulocytes (eosinophilic or basophilic) and 2 groups of agranulocytes (large or small) for performing a cytologic differential. The differential was not significantly altered by anticoagulant treatments providing cell morphology was adequate for obtaining a differential. Eosinophilic granulocytes predominated (59%) with fewer large agranulocytes (27%) and basophilic granulocytes (13%). Small agranulocytes comprised 2% of the total population. Conclusions: No single treatment provided an optimal method to evaluate freshwater mussel hemolymph. Maximal hemocyte counts were obtained following formalin treatment. l ‐cysteine reduced clumping and maintained hemocyte morphology for performing a cytologic differential. These techniques provide a standardized approach for the hematologic evaluation of freshwater mussels. 相似文献
103.
Craig J. Johns Britt A. Holmén Debbie A. Niemeier Robert H. Shumway 《Journal of Agricultural, Biological & Environmental Statistics》2001,6(1):99-117
Dust plumes were generated by farm equipment during standard field preparations and harvest operations. Light detection and ranging (lidar) measurements of dust particle concentrations in the plumes were censored by an upper detection limit. The nonstandardized Gaussian curve, with range values as the argument, was used as a model for the one-dimensional plume profile. Maximum likelihood estimates for the Gaussian plume equation were found in order to make inferences concerning plume characteristics. 相似文献
104.
McDonald D Wu L Bohks SM KewalRamani VN Unutmaz D Hope TJ 《Science (New York, N.Y.)》2003,300(5623):1295-1297
Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that, in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface while the MDDCs concentrated HIV to the same region. We propose that contact between dendritic cells and T cells facilitates transmission of HIV by locally concentrating virus, receptor, and coreceptor during the formation of an infectious synapse. 相似文献
105.
106.
Rolling behaviour of sows in relation to piglet crushing on sloped versus level floor pens 总被引:1,自引:0,他引:1
Lone Danholt Vivi Aarestrup Moustsen Mai Britt Friis Nielsen Anders Ringgaard Kristensen 《Livestock Science》2011,141(1):59-68
The study focused on the rolling behaviour of sows and the crushing of piglets by sows' rolling behaviour. The experiment examined the influence of sloped floor in loose housed farrowing pens on the rolling behaviour of sows and crushing of piglets. The experimental unit was made up of 24 pens. There were two experimental pen designs with piglet creep in the corner of the pen and piglet creep across the end of the pen, respectively. Both of the experimental pen designs had a 10% sloped floor in the sow's resting area. The two control pen designs were identical to the experimental pen designs, but with a level floor. The behaviour of 85 sows and their litters was continuously video recorded. Behavioural observations were made from birth of the first piglet and until 3 days after birth of the first piglet. Rolling behaviour of sows caused significantly more trapped piglets under the sow than lying down from standing (P = 0.04). Rolling behaviour caused 64% of the trapped piglets and lying down from standing caused 36% of the trapped piglets. Rolling from udder to side without protection trapped significantly more piglets than rolling from udder to side near slanted wall or piglet protection rails and rolling from side to udder (P < 0.001). With a certain pen design sloped floor reduced rolling from udder to side without protection (P = 0.007) and reduced the number of trapped piglets (P = 0.01), but results concerning lying behaviour showed that sloped floor pushed sows to rest on the level part of the floor. The results indicate that rolling behaviour that crushes piglets can be reduced, and sows prefer to lie on a level floor. 相似文献
107.
108.
Our understanding of leukemia development and progression has been hampered by the lack of in vivo models in which disease is initiated from primary human hematopoietic cells. We showed that upon transplantation into immunodeficient mice, primitive human hematopoietic cells expressing a mixed-lineage leukemia (MLL) fusion gene generated myeloid or lymphoid acute leukemias, with features that recapitulated human diseases. Analysis of serially transplanted mice revealed that the disease is sustained by leukemia-initiating cells (L-ICs) that have evolved over time from a primitive cell type with a germline immunoglobulin heavy chain (IgH) gene configuration to a cell type containing rearranged IgH genes. The L-ICs retained both myeloid and lymphoid lineage potential and remained responsive to microenvironmental cues. The properties of these cells provide a biological basis for several clinical hallmarks of MLL leukemias. 相似文献
109.
110.
Andreas Bolte Lutz Hilbrig Britt Grundmann Friederike Kampf Jörg Brunet Andreas Roloff 《European Journal of Forest Research》2010,129(3):261-276
It is believed that European beech (Fagus sylvatica L.) will increase its competitive ability at its northern range margin in Scandinavia due to climate change. In mixed old-growth
forests of beech and Norway spruce (Picea abies (L.) Karst.) at Siggaboda nature reserve (southern Sweden), stand structure characteristics were sequentially recorded in
the years 2004, 2005 and 2007 as well as growth in stem diameter using tree-coring analyses. Using these measurements, we
studied the effects on stand dynamics of an extreme storm event (2005 “Gudrun” hurricane), drought and heat (mid-summer 2006,
spring 2007) and subsequent bark beetle attacks on spruce (growing season 2007), overlaid with warming tendencies. The storm,
which caused disastrous damage in many stands nearby, had comparatively little impact on the structure of the spruce–beech
stand. All together, only 32 trees (19 spruces, 10 beeches, 3 other species) per hectare were thrown or broken mainly in the
leeward direction (NE) or impacted by secondary damage by uprooted neighbour trees; this represents 7% of the total tree number
and 11% of the growing stock. Diameter and height structure did not change significantly. However, the 2006 drought and the
2007 attack of biotic agents changed the stand structure and composition strongly due to the death of about 19% of the dominating
older spruce trees that accounted for 35% of total stand volume. This resulted in a considerable increase in beech’s contribution
to stem number (4% increase) and wood volume of the living stand (7% increase). A comparison of diameter growth of beech and
spruce during the periods 1894–1949 and 1950–2005 showed a distinct decrease in growth superiority of spruce during the last
50 years. These results support the idea of a northward migration of European beech as a nemoral tree species in Sweden, due
to a higher tolerance to the abiotic and biotic threats accompanying climate change and an increased competitive ability compared
to boreal tree species Norway spruce. 相似文献