表皮生长因子对牛输卵管上皮细胞生长、增殖和凋亡的影响

研究不同浓度的表皮生长因子对牛输卵管上皮细胞生长、增殖和凋亡的影响,目的是建立高质量的牛体外胚胎共培养细胞系。牛输卵管上皮细胞体外培养技术结合血球计数法检测细胞增殖,并利用流式细胞仪进行细胞周期和凋亡的检测,通过Lysis软件对数据进行分析。结果表明,EGF在0～50 ng/mL浓度范围内,其促增殖、抑制凋亡作用随浓度的增加而增大,呈剂量依赖性;当浓度持续增高时,其促增殖、抑制凋亡能力降低;此外,S期细胞数量明显增加,G2-M期细胞数量有下降趋势。表皮生长因子在一定浓度范围内具有促进牛输卵管上皮细胞增殖和抑制其凋亡的作用,并使其细胞周期发生变化。     

萝卜叶片衰老过程中光合性能变化研究

研究了萝卜品种CR0502和CR0509叶片生长衰老过程中光合性能的变化。结果表明，萝卜叶片展开至衰老过程中，除气孔限制值（Is）随叶片展开时间的延长而缓慢降低外，叶绿素含量、净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）和叶绿素荧光参数（Fv／Fin、ψPSⅡ）的变化趋势一致，呈抛物形，在25d时达到峰值，此后逐渐下降。不同品种间存在明显差异，品种CR0502在叶片衰老期的下降速率明显高于品种CR0509。叶片初展时，气孔和非气孔因素同时影响叶片的Pr，而叶片衰老时，非气孔因素占主要因素。     

ORCHITIS IN TWO DOGS WITH ROCKY MOUNTAIN SPOTTED FEVER

Two dogs with testicular swelling were sonographically diagnosed with orchitis and were subsequently diagnosed with Rocky Mountain spotted fever (RMSF). Use of both gray scale and color Doppler sonography allowed for differentiation of orchitis from neoplasia and torsion. While only experimentally induced RMSF is reported to cause orchitis in dogs, it should be considered in any dog with vascular insult to the testes, especially when other signs of systemic illness are involved.     

犬MC4R基因单核苷酸多态性及其与体重的关系

人和几种动物的遗传学研究显示，MC4R基因在体重和能量平衡调控中具有重要作用，但未见有关犬的MC4R基因多态性、MC4R基因与性状关系的研究报道。以123只成年Beagle犬为试验材料提取血液DNA，利用软件设计PCR引物，经PCR扩增、克隆和测序后，发现了一个新的MC4R基因单核苷酸多态性(single nucleotide polymorphism，SNP)。该多态性恰好产生了限制性内切酶EcoO109Ⅰ的位点多态性。利用PCR-RFLP方法，分析了Beagle犬家系的MC4R基因，并利用SPSS软件分析该多态性与犬体重的关系。结果分析显示：MC4R该多态位点对犬的体重影响不显著。尚需进一步分析MC4R基因的其他片段。     

Progressive tetraparesis and laryngeal paralysis in a young Rottweiler with neuronal vacuolation and axonal degeneration: an Australian case

A 5-month-old female Rottweiler dog was diagnosed to have a neurodegenerative disease that has been recently report ed in Rottweilers from North America and Europe. The dog was presented with progressive signs of ataxia, tetraparesis and inspiratory stridor. The clinical investigation included analysis of CSF, radiography, myelography and electrophysiolog-ical testing. No evidence of vertebral malformation or inflammatory CNS disease was identified. Bilateral laryngeal paraly sis was identified in the lightly anaesthetised dog. Electromyography showed abnormal spontaneous activity from the intrin sic musculature of the larynx. At necropsy there were no gross abnormalities of the nervous system but there was atrophy of the dorsal cricoarytenoid muscles of the larynx. There were widespread histological abnormalities throughout the ner vous system including neuronal vacuolation, spongiform changes in the neuropil and axonal degeneration which was most prominent in the spinal cord. These clinical and pathological findings are consistent with the diagnosis of a new neurode-generative disease reported from North America and Europe. This diagnosis is of particular significance in Australia where transmissible spongiform encephalopathies have not been identified.     

AORTO-CARDIAC FISTULAS IN SEVEN HORSES

This report describes the history, clinical, electrocardiographic and echocardiographic findings, treatment, outcome, and post-mortem findings in seven horses with aorto-cardiac fistula. Affected horses included 5 stallions, one gelding and one mare; 2 each of the Thoroughbred, Arabian and Standardbred breeds and one Thoroughbred-cross with a mean ± s.d. age of 12 ± 4 years, range 6–18 years. The presenting sings were acute distress (four horses), exercise intolerance (two horses) and the lesion was detected during a routine examination in one horse. Five horses had monomorphic ventricular tachycardia on admission and one other had a history of this arrhythmia. Five horses had a characteristic continuous murmur loudes in the right fourth intercostal space. Echocardiography (six horuses) and/or post-mortem examination (four horses) revealed the horses had aorto-cardiac fistulas arising from the right aortic sinus in all five horses in which the site was recorded. Two horses had ruptured aneurysmall dilatations of the aortic wall at this site. Fistulas extended into the right ventricle in four horses; the right atrium in two horses, the left ventricle in one horse, and five horses had dissecting tracts in the septal myocardium. Horses survived for periods ranging from 24th to 4 years. Aorto-cardiac fistula should be considered in the differential diagnosis for horses presenting with acute distress, bounding arterial pulse, a right-sided continuous murmur and/or monomorphic ventricular tachycardia, particularly in middle-aged or older stallions. Echocardiography is the technique of choice for confirming the diagnosis and demonstrating accompanying cardiac changes.     

利用人工卵大量繁殖赤眼蜂及其田间防虫试验概况

利用人工卵大量繁殖赤眼蜂及其田间防虫试验概况刘建峰刘志诚冯新霞李敦松（广东省农科院植物保护研究所,广州５１０６４０）ＰＲＥＳＥＮＴＳＴＡＴＵＳＯＦＭＡＳＳ┐ＲＥＡＲＩＮＧＴＲＩＣＨＯＧＲＡＭＭＡＷＩＴＨＡＲＴＩＦＩＣＩＡＬＨＯＳＴＥＧＧＳＴＯＣＯＮＴ...     

猪新基因CFL2真核表达载体的构建及在C2C12细胞中的瞬时表达

从猪的股二头肌中提取总RNA,利用RT-PCR技术从猪骨骼肌中获得CFL2基因的编码区序列,T-A克隆至PMD-18T载体,经HindⅢ和XhoⅠ酶切后定向克隆至真核表达载体pCDNA3.1(+)中。获得的重组质粒pCDNA3.1(+)/CFL2用限制性内切酶酶切分析和DNA测序分析鉴定,并经脂质体瞬时转染C2C12细胞,最后利用PCR检测转基因细胞中CFL2基因的存在及其表达情况。结果显示:猪CFL2基因已克隆到真核表达载体pCDNA3.1(+)中,转染的C2C12细胞中检测到细胞内较高量CFL2的表达。pCDNA3.1(+)/CFL2真核表达载体的成功构建,为深入研究CFL2基因在猪肌肉细胞中的功能奠定基础,为猪肉质品质的改良提供了新的思路。     

Factors associated with low vitamin D status of Australian alpacas

Objective To investigate factors associated with low vitamin D status of alpacas at pasture in southern Australia. Design A 2‐year survey of alpacas from two farms in South Australia and three in Victoria. Blood samples were collected from 20 to 30 alpacas on each farm on five occasions each year. Breed, gender, age and fleece colour of animals were recorded. Method Blood samples were assayed for plasma 2.5‐hydroxycholecalciferol (25‐OH D₃) and plasma inorganic phosphorus (Pi). Data sets from 802 animal samples were analysed by multiple regression to determine variables associated with low vitamin D status of alpacas. The relationship between plasma 25‐OH D₃ and plasma Pi was also investigated. Results Vitamin D status was significantly affected by month of sampling, with low values in late winter and high values in summer. Plasma vitamin D concentrations increased with age, were higher in alpacas with light fleeces than in those with dark fleeces and were also higher in the Suri than in the Huacaya breed. Plasma Pi concentrations were generally lower in alpacas with plasma 25‐OH D₃ values < 25 nmol/L. Conclusions Young alpacas with dark fleeces are most at risk from vitamin D insufficiency in late winter in southern Australia. The present study indicates that plasma Pi values are not a reliable indicator of vitamin D status of alpacas as assessed by plasma 25‐OH D₃ concentrations.     

SPLENIC SEQUESTRATION SCINTIGRAPHY IN THE DOG: A COMPARISON OF DENATURING TECHNIQUES

Five mixed-breed dogs underwent splenic sequestration scintigraphy following intravenous injection of 647.5 to 740 MBq (17.5–20 mCi) of ^99mTechnetium labeled autologous red blood cells (RBCs) that had been chemically denatured using two μg of stannous chloride. Left lateral dynamic images were obtained for 20 minutes after injection. Regions of interest (ROI) were drawn around the splenic body and ventral extremity, ventral liver and caudal abdominal great vessels and time activity curves created. Count density information was obtained and uptake ratios were calculated for the spleen ROI/vessel ROI, liver ROI/vessel ROI and spleen ROI/liver ROI at 1, 2.5, 5, 10, 15 and 20 minutes after injection. Two additional studies using different RBC denaturing procedures were done in four of the five dogs. In the second study, the stannous chloride level was doubled to 4.0 μg, while in the third study, the RBCs were denatured by addition of 2.0 μg of stannous chloride and heating at 49°C for 15 minutes. Progressive splenic uptake of denatured RBCs was seen in all dogs during the first 15 to 20 minutes of each study, no matter which denaturing technique was utilized. Significant increases in the spleen ROI:/vessel ROI and spleen ROI:liver ROI ratios were obtained at 5, 10, 15 and 20 minutes when compared to the 1 minute values for each of the labeling techniques. A significant difference was not identified between the three ratios at 15 minutes between the three labeling techniques. There was a trend of increasing values for each ratio where 2.0 μg of stannous chloride technique being the lowest, 4.0 μg of stannous chloride having an intermediate value and the heated technique had the highest values. The heating technique resulted in higher liver activity and increased variability of the mean liver ROI/vessel ROI ratios at all times (1, 2.5, 5,10, and 15 minutes). Using a non-linear least squares regression analysis, a double exponential equation fit the spleen ROI/vessel ROI and spleen ROI/liver ROI ratios for all three labeling techniques. Imaging of the spleen using all three techniques was acceptable, and a persistent blood pool image would allow for vascular imaging and cardiac gated studies 30 minutes after injection of the denatured, labeled autologous red blood cells. Veterinary Radiology & Ultrasound, Vol. 36, No. I, 1995, pp 57–63.