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Lezcano M Novo M Al-Soufi W Rodríguez-Núñez E Tato JV 《Journal of agricultural and food chemistry》2003,51(17):5036-5040
The formation of inclusion complexes with beta-cyclodextrin was studied for several popular fungicides of different types: prochloraz, 2-phenylphenol, thiophanate methyl, 8-hydroxyquinoline, and benalaxyl. Phase solubility diagrams showed that in all cases complexation takes place, leading to an important increase of water solubility in prochloraz and benalaxyl. Equilibrium association constants could be determined from the phase solubility data and from NMR titrations in the case of 2-phenylphenol. Because of the low solubility of the complex formed between 8-hydroxyquinoline and beta-cyclodextrin, the corresponding association constant could not be determined. The solid complexes of fungicide-cyclodextrin were prepared and isolated by different methods. The isolation of real complexes and not physical mixtures was confirmed in the cases of prochloraz, 2-phenylphenol, and benalaxyl by differential scanning calorimetry. 相似文献
63.
Palazolo G Rodríguez F Farruggia B Picó G Delorenzi N 《Journal of agricultural and food chemistry》2000,48(9):3817-3822
Functional properties of whey protein concentrates (WPC) are primarily dependent on the degree of denaturation of beta-lactoglobulin (beta-LG), the major globular whey protein. Irreversible modifications in the tertiary structure and association state of beta-LG after heat treatment were studied by partition in aqueous two-phase systems and fluorescence quenching. Partitioning of preheated beta-LG in two-phase systems containing 5% (w/w) poly(ethylene glycol) and 7% (w/w) dextran, between pH 6.0 and7.0, are appropriately related with the intensity of heat treatment. An increase in the partition coefficient of beta-LG was observed with increasing temperature of heat treatment. On the other hand, fluorescence quenching of beta-LG by acrylamide was used to study the conformational flexibility of the protein at pH values between 4. 0 and 9.0. The values of bimolecular quenching rate constant (k(q)) obtained showed that beta-LG appears to be more flexible at high pH values, while at low pH the protein assumes a more compact form. The efficiency of acrylamide quenching on preheated beta-LG was substantially more pronounced than for the untreated protein. This difference can be ascribed to the presence of unfolded monomers and aggregates of denatured molecules formed after heat treatment, whose tryptophanyl residues are more exposed to the solvent. In conclusion, the results suggest that partition studies in aqueous two-phase systems and fluorescence quenching are very useful tools to detect changes in conformation and aggregation of beta-LG induced by heat treatment. 相似文献
64.
Miguel Esquivel Leonor Castiñeiras Bartolomé Rodríguez Karl Hammer 《Genetic Resources and Crop Evolution》1987,35(2):367-378
Summary After a short introduction into recent collecting activities in Cuba information is provided concerning the joint expedition of the Instituto de Investigaciones Fundamentales en Agricultura Tropical and the Zentralinstitut für Genetik und Kulturpflanzenforschung in the province of Holguín, Eastern Cuba, in February 1986 within the agreement between the Academy of Sciences of Cuba and the Academy of Sciences of the German Democratic Republic. Altogether 249 accessions of vegetables, pulses, cereals and other crops were collected. A remarkable diversity was found inCapsicum andPhaseolus. The material collected is important for breeding purposes.
Sammlung pflanzlicher genetischer Ressourcen in Kuba
Zusammenfassung Nach einer kurzen Einführung in die Sammelarbeit der letzten Jahre in Kuba folgen Informationen über die gemeinsame Expedition des Instituto de Investigaciones Fundamentales en Agricultura Tropical und des Zentralinstituts für Genetik und Kulturpflanzenforschung, die im Rahmen des Abkommens zwischen der Akademie der Wissenschaften Kubas und der Akademie der Wissenschaften der Deutschen Demokratischen Republik im Februar 1986 durchgeführt wurde und in die Provinz Holguín, Ost-Kuba, führte. Insegsamt wurden 249 Proben von Gemüsen, Hülsenfrüchten, Getreiden und anderen Fruchtarten gesammelt. Eine bemerkenswerte Variation wurde beiCapsicum andPhaseolus gefunden. Das Sammelmaterial ist für Züchtungszwecke von Bedeutung.
¶rt; , , , ¶rt; . C 1986 . . 249 , , . Capsicum Phaseolus. .相似文献
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Adsorption of whey protein isolate at the oil-water interface as a function of processing conditions: a rheokinetic study 总被引:4,自引:0,他引:4
Rodríguez Patino JM Rodríguez Niño MR Sánchez CC 《Journal of agricultural and food chemistry》1999,47(6):2241-2248
In this paper we present surface dynamic properties (interfacial tension and surface dilational properties) of a whey protein isolate with a high content of beta-lactoglobulin (WPI) adsorbed on the oil-water interface as a function of adsorption time. The experiments were performed at constant temperature (20 degrees C), pH (5), and ionic strength (0.05 M). The surface rheological parameters and the interfacial tension were measured as a function of WPI concentration (ranging from 1 x 10(-)(1) to 1 x 10(-)(5)% w/w) and different processing factors (effect of convection and heat treatment). We found that the interfacial pressure, pi, and surface dilational modulus, E, increase and the phase angle, phi, decreases with time, theta, which should be associated with WPI adsorption. These phenomena have been related to diffusion of the protein toward the interface (at short adsorption time) and to the protein unfolding and/or protein-protein interactions (at long-term adsorption) as a function of protein concentration in solution and processing conditions. 相似文献
67.
Supercritical fluid extraction of pesticides from a table-ready food composite of plant origin (gazpacho) 总被引:1,自引:0,他引:1
Aguilera A Brotons M Rodríguez M Valverde A 《Journal of agricultural and food chemistry》2003,51(19):5616-5621
Supercritical fluid carbon dioxide extraction (SFE) has been evaluated for the extraction of 17 organohalogen and organophosphate pesticides in gazpacho (a table-ready food composite containing crude vegetables, white bread, vegetable oil, water, and other minor components) using anhydrous magnesium sulfate as drying agent. The effects of different parameters, such as fat content in gazpacho composites, magnesium sulfate/gazpacho ratio, supercritical fluid volume, pressure, temperature, and static modifier additions, on SFE recoveries from spiked gazpacho samples have been studied. Analyses were performed by gas chromatography (GC) with flame photometric (FPD), electron capture (ECD), and mass spectrometry (MSD) detectors. In most experiments, recoveries obtained for the nonpolar organohalogen pesticides were lower than those obtained for the most polar organophosphate pesticides, but overall pesticide recoveries determined by using the optimal SFE conditions indicate that SFE could be used to determine pesticide residue levels in gazpacho. 相似文献
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