Genetic parameters for spawning and growth traits in the Pacific white shrimp (Penaeus (Litopenaeus) vannamei)

The aim of this study was to estimate genetic parameters for spawning traits and growth traits in a breeding line of Pacific white shrimp, Penaeus (Litopenaeus) vannamei, selected for growth and survival. Traits studied were number of eggs (NE) and number of nauplii (NN) and female body weight at insemination (FWI) and body weight at 130 days of age (BW130). Genetic parameters were estimated using a multivariate animal model. Heritability for NE and NN were estimated as 0.13 ± 0.04 and 0.03 ± 0.04 respectively. The contribution to NN total variation due to ‘factors associated with male’ effect was estimated as 0.47 ± 0.07. In the cases of FWI and BW130, heritability was estimated as 0.44 ± 0.08 and 0.19 ± 0.03 respectively. Genetic correlation between FWI and NE was estimated as 0.49 ± 0.15, between FWI and NN as 0.54 ± 0.39 and between NE and NN as 0.27 ± 0.41, whereas the genetic correlations of FWI, NE and NN with BW130 were 0.30 ± 0.13, ?0.21 ± 0.19 and ?0.25 ± 0.38 respectively. Although it is important to perform more studies on this issue, our results found no evidence of a genetic antagonistic effect between female reproductive traits and body weight at harvesting (130 days of age) in P. vannamei.     

Production of Surface-Active Compounds by a Hydrocarbon-Degrading Actinobacterium: Presumptive Relationship with Lipase Activity

The replacement of synthetic surface-active compounds (SACs) by their microbial counterparts is carving out a niche for themselves in the field of bioremediation. However, the high cost of microbial products has limited their application at a realistic scale. In the current study, several hydrocarbon-degrading microorganisms were assayed as potential SAC producers in low-cost liquid media. Only the strain CC10, placed within the class Actinobacteria, was able to produce emulsifying molecules by using a combination of sugarcane vinasse or crude glycerol (as cheap carbon substrates) with urea or peptone (as nitrogen sources). The emulsifying activity of the supernatants and the stability of emulsions formed with motor oil depended on the carbon and nitrogen sources. However, the biodegradability of these metabolites was only associated with the carbon substrate, and it was always higher than the two tested synthetic surfactants, sodium dodecyl sulfate and Triton X-100. Also, a positive linear association between emulsifying and lipase activities of the CC10 supernatants was detected (r?=?0.781; p?=?0.219), with the maximum activities detected in the glycerol-peptone supernatant. Interestingly, this supernatant was able to emulsify different oily substrates, a property that could be used to increase the efficiency of the treatment of effluents with high fat content.     

Signatures of adaptation to obligate biotrophy in the Hyaloperonospora arabidopsidis genome

Many oomycete and fungal plant pathogens are obligate biotrophs, which extract nutrients only from living plant tissue and cannot grow apart from their hosts. Although these pathogens cause substantial crop losses, little is known about the molecular basis or evolution of obligate biotrophy. Here, we report the genome sequence of the oomycete Hyaloperonospora arabidopsidis (Hpa), an obligate biotroph and natural pathogen of Arabidopsis thaliana. In comparison with genomes of related, hemibiotrophic Phytophthora species, the Hpa genome exhibits dramatic reductions in genes encoding (i) RXLR effectors and other secreted pathogenicity proteins, (ii) enzymes for assimilation of inorganic nitrogen and sulfur, and (iii) proteins associated with zoospore formation and motility. These attributes comprise a genomic signature of evolution toward obligate biotrophy.     

SARS-CoV-2 natural infection in animals: a systematic review of studies and case reports and series

COVID-19 pandemic is essentially a zoonotic disease. In this context, early in 2020, transmission from humans to certain animals began reporting; the number of studies has grown since. To estimate the pooled prevalence of SARS-CoV-2 natural infection in animals and to determine differences in prevalence between countries, years, animal types and diagnostic methods (RT-PCR or serological tests). A systematic literature review with meta-analysis using eight databases. Observational studies were included but analyzed separately. We performed a random-effects model meta-analysis to calculate the pooled prevalence and 95% confidence interval (95% CI) for prevalence studies and case series. After the screening, 65 reports were selected for full-text assessment and included for qualitative and quantitative analyses. A total of 24 reports assessed SARS-CoV-2 infection by RT-PCR, combining a total of 321,785 animals, yielding a pooled prevalence of 12.3% (95% CI 11.6%–13.0%). Also, a total of 17 studies additionally assessed serological response against SARS-CoV-2, including nine by ELISA, four by PRTN, one by MIA, one by immunochromatography (rest, two studies, the method was not specified), combining a total of 5319 animals, yielding a pooled prevalence of 29.4% (95% CI 22.9%–35.9%). A considerable proportion of animals resulted infected by SARS-CoV-2, ranking minks among the highest value, followed by dogs and cats. Further studies in other animals are required to define the extent and importance of natural infection due to SARS-CoV-2. These findings have multiple implications for public human and animal health. One Health approach in this context is critical for prevention and control.     

Morphological comparison of wild,farmed and hybrid specimens of two South American silversides,Odontesthes bonariensis and Odontesthes hatcheri

In this study, body shape of hybrid and presumptive introgressed South American silversides was studied. Body shape of O. bonariensis and O. hatcheri from wild populations and farmed stocks was compared to provide basic information on the effects of fish farming on morphometric parameters. Subsequently, wild presumptive introgressed individuals and artificially hybridized farmed individuals were morphologically analysed to assess the effects of hybridization on the same parameters. Most farmed purebred individuals were shorter and higher than their wild counterparts, which is probably due to the favourable growth conditions compared to the wild habitat. However, the results evidenced that purebred individuals were more slender than both hybrid (farmed) fish and introgressed (wild) fish. Further studies on the growth performance of hybrid Odontesthes will be required in order to assess whether the combination of hybridization and sterilization could produce, under farming conditions, growth performances which satisfy the requirements of aquaculture.     

Effect of the US3 protein of bovine herpesvirus 5 on the actin cytoskeleton and apoptosis

The US3 protein is a unique protein kinase only present in the Alphaherpesvirinae subfamily of the herpesviruses. Studies performed with several alphaherpesviruses demonstrated that the US3 protein is involved in cytoskeleton modifications during viral infection and displays anti-apoptotic activity. However, the US3 protein of BoHV-5 has not been studied up to now. As reported for other alphaherpesviruses, our results showed that BoHV-5 US3 confers resistance against apoptosis and induces cytoskeletal reorganization leading to cell rounding, actin stress fiber breakdown and cell projections that interconnect cells. The expression of a kinase-dead version of BoHV-5 US3 showed that the anti-apoptotic activity and the induction of cell projections are kinase-dependent whereas kinase activity is not absolutely required for actin stress fiber breakdown. Besides, the kinase-dead version of US3, but not the wild type protein, was found excluded from the nucleus. These results constitute the first report on the BoHV-5 US3 functions, and highlight that there are functional differences and similarities among US3 proteins of different alphaherpesviruses.     

N<Subscript>2</Subscript>-fixation along a gradient of long-term disturbance in tropical mangroves bordering the gulf of Mexico

Microbial processes are key elements in determining the productivity of mangroves, and reductions in these processes reflect the loss of microbial biodiversity and function due to fabricated disturbances. Because nitrogen is a major limiting nutrient for the productivity of these ecosystems, the goal of this study was to determine profiles of inorganic nitrogen combined with several environmental parameters, all in relation to the degree of long-term hydraulic impairment of a tropical, monospecific black mangrove (Avicennia germinans) forest that showed degradation ranging from total loss of mangrove cover to no disturbance. N₂-fixation, oxygen levels, and nitrite contents decreased significantly with the severity of the disturbance, and almost null levels were reached in the completely degraded zone, whereas salinity achieved very high values. Concomitantly, total N, ammonium, and P contents and ammonia volatilization increased significantly. Pore-water temperature and pH increased moderately. Other soil physical properties (sand, silt, clay, organic matter, and total C), which varied among the sampling sites, were not correlated with the level of disturbance. Principal component analyses, including environmental and biological parameters, suggested that the most significant finding was the considerable loss of N₂-fixation with increasing impairment, which was concomitant with significant increases in volatilization of ammonia and salinity. The results show that microbial N-cycling processes are highly sensitive to salinity and to man-made disturbances that modify the water level and flow.     

ADVERSE EFFECTS OF HUMIC SUBSTANCES FROM DIFFERENT ORIGIN ON LUPIN AS RELATED TO IRON SOURCES

Humic substances improve the efficiency of different iron (Fe) sources overcoming Fe deficiency chlorosis of plants. However, applied at high rates, they can promote negative effects on plants. The main objective of this work was to study the potential adverse effect of three humic acids from different origin when they were applied with two effective Fe sources for plants: Fe- ethylenediaminedihydroxyphenylacetic acid (EDDHA) and Vivianite. To this end, an experiment with lupin (Lupinus albus L.) was performed involving two factors: (i) Fe source, and (ii) humic substances from three different origin (composted cork, leonardite, and compost obtained from a mixture of olive husk with cotton gin trash) applied at 0, 0.1, and 0.5 g organic carbon (C) kg^?1 of growing media. At the rates used, humic substances promoted adverse effects on plant development, chlorophyll meter readings, and Fe content in lupin grown in calcareous media. Overall, the effect on dry matter and Fe content in plants was more relevant when Fe was supplied with Vivianite, the effect on chlorophyll meter readings being more significant when Fe was applied as Fe-EDDHA. Differences were also observed depending on the source of humic substances, those from leonardite promoting the greatest decrease in dry matter in roots and shoots. These humic substances possessed the highest values of spectroscopy index for aromaticity (A₂₅₄ ). On the other hand, the application of humic substances from olive husk compost, which exhibited the lower aromaticity index, resulted in the smallest decrease in dry matter production and chlorophyll meter readings. Dry matter in roots decreased logarithmically with increased values of the estimates of the amounts of aromatic compounds accumulated in the growing media (R² = 0.92; P < 0.01) with Vivianite as Fe source. Thus, the effects decreasing dry matter production, particularly in roots, and chlorophyll meter readings can be ascribed at least partially to the presence of phytotoxic aromatic compounds in humic substances.     

Ontogeny and total sugar content of yacon tuberous roots and other three Smallanthus species (Heliantheae,Asteraceae), insights on the development of a semi-domesticated crop

The ontogeny of the tuberous roots of yacon (Smallanthus sonchifolius), an ancient Andean crop, is described for the first time along with three other wild species of Smallanthus: S. connatus, S. macroscyphus and S. siegesbeckius. Tuberous storage roots are present in the four species. Tuberization results from unusual secondary thickening. A meristematic endodermis maintains the primary cortex by generating cortex cells continuously. Secretory schizogenous canals develop adjacent to the endodermis in opposition to the phloem poles. Phellogen originates in the outer layers of the parenchymatic cortex. The four species develop a parenchymatous pith that stores mainly fructooligosacharides (FOS) and plays an important role in storing water, protecting the plant from drought stress and environmental hazards in the Andean climate. In yacon, domestication has produced a wide pith that stores water, reducing sugars and FOS with low degree of polimerization, that could have eventually made it more palatable as compared to the wild counterparts.