Atomic-scale visualization of inertial dynamics

The motion of atoms on interatomic potential energy surfaces is fundamental to the dynamics of liquids and solids. An accelerator-based source of femtosecond x-ray pulses allowed us to follow directly atomic displacements on an optically modified energy landscape, leading eventually to the transition from crystalline solid to disordered liquid. We show that, to first order in time, the dynamics are inertial, and we place constraints on the shape and curvature of the transition-state potential energy surface. Our measurements point toward analogies between this nonequilibrium phase transition and the short-time dynamics intrinsic to equilibrium liquids.     

Structural basis for gluten intolerance in celiac sprue

Celiac Sprue, a widely prevalent autoimmune disease of the small intestine, is induced in genetically susceptible individuals by exposure to dietary gluten. A 33-mer peptide was identified that has several characteristics suggesting it is the primary initiator of the inflammatory response to gluten in Celiac Sprue patients. In vitro and in vivo studies in rats and humans demonstrated that it is stable toward breakdown by all gastric, pancreatic, and intestinal brush-border membrane proteases. The peptide reacted with tissue transglutaminase, the major autoantigen in Celiac Sprue, with substantially greater selectivity than known natural substrates of this extracellular enzyme. It was a potent inducer of gut-derived human T cell lines from 14 of 14 Celiac Sprue patients. Homologs of this peptide were found in all food grains that are toxic to Celiac Sprue patients but are absent from all nontoxic food grains. The peptide could be detoxified in in vitro and in vivo assays by exposure to a bacterial prolyl endopeptidase, suggesting a strategy for oral peptidase supplement therapy for Celiac Sprue.     

Titan: discovery of carbon monoxide in its atmosphere

The 3-0 rotation-vibration band of carbon monoxide in the near-infrared spectrum of Titan has been identified, and a reflecting layer model mixing ratio of carbon monoxide to molecular nitrogen of 6 x 10(-5) has been determined. This result supports the probable detection of carbon dioxide by Samuelson and his co-workers and strengthens possible analogies between the atmosphere of Titan and conditions on primitive Earth.     

The age and size of the universe

Modern distance determinations to galaxies were reviewed and placed on a uniform and self-consistent scale. Based on eight separate but not entirely independent techniques, the distance to the Virgo cluster was found to be 15.8 +/- 1.1 megaparsec. Twelve different determinations yield a Coma/Virgo distance ratio of 5.52 +/- 0.13 and hence a Coma distance of 87 +/- 6 megaparsec. With a cosmological redshift of 7210 kilometers per second, this gives a Hubble parameter H(0) (local) of 83 +/- 6 kilometers per second per megaparsec. From the velocity-distance relation of rich clusters of galaxies, the ratio of the value of H(0) (global) to the value of H(0) (local) was determined to be 0.92 +/- 0.08. In other words, the cluster data do not show a statistically significant difference between the local and global values of the Hubble parameter. If one nevertheless adopts this relation between H(0) (global) and H(0) (local), then the value of H(0) (global) is 76 +/- 9 kilometers per second per megaparsec. This observed value differs at the approximately 3sigma level (where sigma is the standard deviation of the distribution) from values in the range 36 less, similar H(0) less, similar50 kilometers per second per megaparsec, which are derived from stellar evolutionary theory in conjunction with standard cosmological models with a density parameter (Omega) that is equal to 1 and a cosmological constant (lambda) that is equal to 0.     

The Chironomid (Diptera) Subfossil Record of Lille Hovvatn (Norway), with a Comparison with a Similar Study in Adjacent Store Hovvatn

The subfossil chironomid community of Lille Hovvatn during the last 150 years was analysed. A short sediment core was taken in 1999, sliced at 0.5 cm intervals and dated using the ²¹⁰Pb method. A total of 1588 specimens were identified, most of them to species level, and 37 chironomid taxa were found. Effects of acidification were recorded on several species up-core, especially in the distribution of Micropsectra insignilobus and Stictochironomus rosenshoeldi. Ordination analysis showed that the chironomid community of Lille Hovvatn is different from the one in Store Hovvatn based on the abundance of the various species, but that the species composition is almost identical in the two lakes. The reappearance of S. rosenshoeldi in the upper 1 cm of the sediment in Lille Hovvatn probably is the first response among the chironomids to the increased pH values measured in the lake during the last decade.     

A single-dose pharmacokinetic study of oxolinic acid and vetoquinol,an oxolinic acid ester,in cod,Gadus morhua L., held in sea water at 8 degrees C and in vitro antibacterial activity of oxolinic acid against Vibrio anguillarum strains isolated from diseased cod

The pharmacokinetic properties of the antibacterial agent oxolinic acid and vetoquinol, the carbitol ester of oxolinic acid, were studied after intravenous (i.v.) and oral (p.o.) administration to 100-150 g cod, Gadus morhua L., held in sea water at 8 degrees C. Following i.v. injection, the plasma drug concentration-time profile showed two distinct phases. The distribution half-life (t1/2alpha) was estimated at 1.3 h, the elimination half-life (t1/2beta) as 84 h and the total body clearance (Cl(T)) as 0.047 L kg(-1) h(-1). The volume of distribution at steady state, Vd(ss) was calculated to be 5.5 L kg(-1), indicating good tissue penetration of oxolinic acid in cod. Following p.o. administration of oxolinic acid or vetoquinol, the peak plasma concentrations (C(max)) of oxolinic acid and the time to peak plasma concentrations (T(max) were estimated to be 1.2 and 2.5 microg mL(-1) and 24 and 12 h, respectively. The bioavailabilities of oxolinic acid following p.o. administration of oxolinic acid and vetoquinol were calculated to be 55 and 72%, respectively. The in vitro minimum inhibitory concentration (MIC) values of oxolinic acid against three strains of Vibrio anguillarum isolated from diseased cod were 0.016 microg mL(-1) (HI-610), 0.250 microg mL(-1) (HI-618) and 0.250 microg mL(-1) (HI-A21). Based on a MIC value of 0.016 microg mmL(-1) a single p.o. administration of 25 mg kg(-1) of oxolinic acid maintains plasma levels in excess of 0.064 microg mL(-1), corresponding to four times the MIC-value, for approximately 12 days. The analogous value for a single p.o. dose of 25 mg kg(-1) of oxolinic acid administered as vetoquinol was 13 days.     

Teratogenicity of elevated egg incubation temperature and egg vitamin A status in Atlantic salmon, Salmo salar L

The present study was undertaken to investigate the possibility that high egg vitamin A (VA) status in combination with elevated egg incubation temperatures may cause deformities in Atlantic salmon, Salmo salar L. Egg batches selected for their total VA concentration were exposed to low (normal, 8 degrees C) or elevated (14 degrees C) egg incubation temperatures. Temperature was the main factor causing bone deformities such as warped gill opercula, fin and jaw deformities, but not for the development of spinal deformities where all groups displayed a 'baseline' occurrence of mild deformity (decreased vertebral size in the cephalic region) and no systematic variation in the occurrence of serious spinal deformities (fused vertebrae). A possible effect of egg incubation temperature fluctuation was found for the groups reared at low temperatures. An indication of a negative effect of elevated egg VA status for the development of organ deformities such as missing septum transversum and situs inversus was found in addition to temperature effects, however, no firm conclusions could be drawn from the present data. The phenotypes for temperature-induced deformities resembled the phenotype of VA-induced deformities, but no clear conclusions on the causality of the deformities found in the present study could be drawn. Egg incubation temperatures, both absolute temperature and temperature variations, should therefore be strictly controlled.