Two-dimensional Electrophoresis Analysis of Proteins in Response to Cold Stress in Extremely Cold-resistant Winter Wheat Dongnongdongmai 1 Tillering Nodes

The overwintering survival ratio of the cultivar Dongnongdongmai 1 with strong cold-resistance in paramos of Heilongjiang Province in China are over 85%. The tillering nodes are the most important organs for overwintering survival of winter wheat, because there are more substances associated with cold resistance in tillering nodes than those in leaves and roots. Proteins in the tillering nodes of the cold-resistant cultivar Dongnongdongmai 1 grown under field conditions with or without any low-temperature stress were analyzed by 2–dimensional electrophoresis and identified by mass spectrometry. In the range of pH 4–7, the expression of 37 proteins showed obvious difference (±more than two fold) in the proteomic maps of cold-stressed and non-stressed tillering nodes, including a new protein spot. All proteins exhibiting the difference in expression were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, followed by a database search for protein identification and function prediction. Five groups of proteins were confirmed, namely stress-related proteins (22%), metabolism-associated proteins (35%), and signaling molecules (24%), cell wall-binding proteins (5%), unclear proteins (14%). This indicated that tillering node cells supported the energy requirements of plant growth and stress resistance by signal transduction adapting to metabolism and structure.     

Evergreen broad-leaved forest improves soil water status compared with tea tree plantation in Ailao Mountains,Southwest China

Abstract

In this paper, the spatial-temporal dynamics of soil moisture content was investigated in an evergreen broad-leaved forest and a tea tree plantation in Ailao Mountains, which was dominated by Fagaceae (Castanopsis wattii and Lithocarpus xylocarpus). Soil moisture content was studied between January 2005 and December 2006 at different depths (from 0–150 cm) with a neutron probe. The results showed that mean soil moisture content in the evergreen broad-leaved forest was usually higher than in the tea tree plantation in the dry season, whereas it was lower than the tea tree plantation in the rainy season. In addition, mean soil moisture content was depth dependent, and in the 10–50 cm layer the spatial variability was due to the active root zone within this depth area in two types of land use. From 50–150 cm, the spatial variability was slightly increasing in the evergreen broad-leaved forest or relatively stable in the tea tree plantation. Our study also showed that soil moisture content was higher and more stable under the evergreen broad-leaved forest than the tea tree plantation, hence we stress that evergreen broad-leaved forest plays an important role in holding soil moisture. It is suggested that the protection of evergreen broad-leaved forest should be strengthened.     

切实加强森林资源管理 提高凭证采伐率

凭证采伐率是体现一个地方森林资源管理的重要指标，文章对重点林区凭证采伐率的现状及下降原因作了分析，提出提高凭证采伐率的对策，这对森林采伐限额管理有一定的参考价值。     

浮顶油罐大修中金属结构的涂装

浮顶罐大修中，金属结构的涂装工作量大，强度高，制定合理的朋修方案，选择适用的表面预处理方式，编制细致的涂装工序，是提高油罐大修的工作效率和保证施工质量的有效措施。     

浅谈大扩孔桩施工技术

本文详尽地叙述了大扩孔桩在基础施工中应采取的施工技术。     

种瓣及密度对大蒜产量的影响

采用二次正交通用旋转设计，进行了种辩大小与种植密度对大蒜产量影响的研究，建立了大蒜产量与种植密度及种瓣大小间的二元二次数学模型。通过模型解析得出，种植密度对产量的影响大于种瓣大小，但密度对单株产量的贡献为负值。通过计算机模拟试验，得出本试验条件下，获得３００００ｋｇ／ｈｍ２以上产量的种植密度为５２．９５～６３．４５万株／ｈｍ２、种瓣大小为４．９～７．０ｇ;;单株产量大于５０ｇ的种植密度为３４．０５～４．５５万株／ｈｍ２、种瓣大小４．９～７．０ｇ。     

谈中小桥采用后张法16m预应力空心板的施工问题

本文阐述了中小桥上部结构采用后张法１６ｍ预应力空心板的施工时应注意的问题，如预应力空心板的预制中底模的施工；侧模、蕊模；砼的要求；浇筑空心板的具体步骤。     

玉米品种贮藏期对玉米象抗性的初步研究

国内外在粮食作物田间生长期间抗虫性的研究开展较早,并取得了显著的成效。但对收获后贮藏期间品种抗虫性的研究较少。Fermando(1959)研究了稻谷品种在贮藏期间对麦蛾的抗性之后,应用抗虫品种作为防治仓虫的一项经济有效的手段,开始为人们所重视,继后有小麦、大豆、豌豆、芝麻等品种抗仓虫的研究报道。Pandgy等     

粟品种生化物质对粟芒蝇的影响

近年来粟芒蝇(Atherigona biseta Karl)在我国北方谷子产区的为害日趋严重,已成为夏谷生产的严重障碍。中国是谷子的原产地,品种资源极为丰富,抗虫性研究利用的潜力很大,但对谷子抗粟芒蝇的内在机制,尤其是谷株体内一些生化物质在抗虫中的