Development of a label-free immunosensor system for detecting oocyte maturation-inducing hormone in fish

A label-free immunosensor for detecting the oocyte maturation-inducing hormone 17, 20β-dihydroxy-4-pregnen-3-one (DHP), was developed. A principle of the sensor system was based on differences in electrochemical activity changed by an immunoreaction in the absence and presence of DHP. For preparation of the immunosensor, anti-DHP IgG was immobilized on an Au working electrode modified with a self-assembled monolayer of 3-mercaptopropionic acid. The sensor was immersed into a sample solution containing DHP. DHP was determined by cyclic voltammetry. The immunosensor showed a specific response to DHP, and the oxidation peak current linearly decreased in the range of 7.8–500 pg ml⁻¹ with a correlation coefficient of 0.996. The sensor system was applied to determine the DHP levels in plasma of goldfish and was compared with the DHP levels of the same samples determined using an ELISA as the conventional method. Good correlation was obtained between values determined using both methods in the range of 0.1–7.7 ng ml⁻¹ (correlation coefficient 0.876). These findings suggest that the proposed label-free immunosensor can be used to analyze DHP levels in fish plasma samples.     

Effect of salt stress on the physiological and photosynthetic characteristics of Weigela florida

Effects of salt stress on the physiological and photosynthetic characteristics of Weigela florida were studied. The results showed that the leaf area of Weigela florida was enhanced at 0.1% and 0.2% salt concentration, but decreased obviously when salt concentration was higher than 0.3%. The symptom of the salt injury was not significant when the salt concentration was lower than 0.3%, but was significant when higher than 0.4%. The water content decreased gradually but the electrolyle leakage increased gradually with the increasing of salt concentration and time. The total content of chlorophyll was rising while salt concentration was lower than 0.3%, but decreased while salt concentration was higher than 0.4%. The proline contents increased gradually while salt concentration was lower than 0.5%, but decreased at 0.5%. The soluble sugar content increased gradually with the increasing of salt concentration and prolonging of treatment, but decreased at 0.4% salt concentration 15 days after salt treatment. To the photosynthetic characteristics, the salt concentrations of 0.1% and 0.2% did not affect the photosynthetic characteristics of Weigela florida, when salt concentration was higher than 0.3%, the salinity significantly reduced Pn, Gs and Tr, but enhanced intercellular CO₂ concentrations at the salt concentrations of 0.3% and 0.4%. It was indicated that 0.1% and 0.2% salt concentrations had little influence on Weigela florida. The growth of Weigela florida was slightly decreased at the salt concentration of 0.3% without effect on its normal growth. However, the salt concentration of 0.4% affected the growth of Weigela florida obviously. Weigela florida died 7 days after treatment and it could not normally grow when salt concentration is higher than 0.5%.     

氰烯菌酯与己唑醇及其混配对小麦赤霉病菌的影响

为明确氰烯菌酯与己唑醇不同比例混配对小麦赤霉病菌联合作用类型,采用菌丝生长速率法测定了氰烯菌酯与己唑醇及其6种配比对小麦赤霉病菌的毒力。结果表明,氰烯菌酯与己唑醇及1:1、1:3、1:5、1:7、1:9、1:11等混配组合对小麦赤霉病菌的EC50分别是0.393、0.643、0.571、0.338、0.369、0.513、0.577、0.726 mg/L;6种混配组合对小麦赤霉病菌的增效系数（SR）分别是0.85、1.64、1.58、1.16、1.05、0.84。氰烯菌酯对小麦赤霉病菌的室内生物活性高于己唑醇,氰烯菌酯与己唑醇1:3和1:5配比对小麦赤霉病菌有增效作用。     

干旱胁迫对甜叶菊生理指标及单株干叶产量的影响

为了给干旱胁迫条件下的甜叶菊栽培管理提供理论依据,利用温室盆栽法研究了干旱胁迫对不同种质材料甜叶菊生理指标及干叶产量的影响。结果表明:正常水分处理下5份甜叶菊种质材料的可溶性蛋白质含量、相对电导率、丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)及甜叶菊单株干叶产量不同。随着干旱时间的持续,5份甜叶菊种质材料的可溶性蛋白质含量均表现为下降趋势,仅IS-1在T3(停止灌溉15天)略有上升;5份甜叶菊种质材料相对电导率均出现较平稳的显著增加;IS-1和AL-4叶片MDA的含量随着干旱胁迫时间的持续先降低后升高,而QF-1、‘青甜杂4’及JD-1叶片MDA的含量持续升高;SOD与POD的活性均表现出先升高后降低再升高的变化趋势;5份甜叶菊种质材料的单株干叶产量均出现不同程度的下降趋势。相同处理中不同材料的相同指标变化程度不同,说明其抗旱能力不同:其中IS-1最强,QF-1最弱,其他3份材料的抗旱性顺序分别为:AL-4>‘青甜杂4’>JD-1。     

强优势小麦杂交种‘绵杂麦168’选育研究

为利用小麦杂种优势提高产量、改进品质和抗性,采用“四同步一交叉”育种技术和方法,利用具有自主知识产权的温光型两系不育系MTS-1与恢复系MR168配组,育成早熟,穗大粒多,结实正常,高抗条锈病和白粉病,产量高,品质优良,适应性广的杂交小麦新品种‘绵杂麦168’通过国家和省级双审定。总结了‘绵杂麦168’的选育历程,配套栽培技术研究和高产栽培、繁育技术措施;简述了‘绵杂麦168’在区域试验和示范推广中的突出表现,认为‘绵杂麦168’是中国小麦杂种优势利用研究取得的重要成果,具有广阔的推广应用前景。     

拮抗细菌bio-2的抑菌蛋白初步研究

拮抗细菌bio-2(Bacillus subtilis)经发酵、离心、硫酸铵沉淀、浓缩和过滤,获得蛋白粗提物;将蛋白粗提物经60C、80℃、100℃、120℃处理后测定其对稻瘟病菌的抑菌活性,结果显示,蛋白粗提物经60℃和80℃处理后,其活性并没有明显降低,经100％处理后,其活性显著下降,而经120℃处理后,蛋白粗提...     

中国胶合板出口美国遭遇的非关税贸易壁垒及其影响分析

随着林产品生产规模的扩大和出口份额的提高, 中国林业企业遭遇越来越多的出口贸易壁垒, 尤其是在作为我国胶合板出口第1大市场的美国。这不仅阻碍了我国林业产业的发展, 甚至严重威胁一部分企业的生存。文中利用量化分析工具测算出, 在美国消费者偏好中国胶合板产品的情况下, 中国胶合板面临的非关税贸易壁垒相当于150%到580%的从价关税税率。面对严峻的形势, 我国企业应在提高自身国际竞争能力的同时, 积极应对贸易摩擦, 并着力开拓新市场。     

Habitat suitability,coverage by protected areas and population connectivity for the Siamese crocodile Crocodylus siamensis Schneider, 1801

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