Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.     

Sensitivity of four Yeasts to Fungicides and CO2 Concentrations and Their Antagonistic Ability in Combination with Fungicide to pathogenic Fungi in vitro

The yeasts Cryptococcus laurentii,Trichosporon pullulans,Rhodotorula glutinis and Trichosporon sp.were used to investigate their sensitivity to four fungicides and different concentrations of CO2,as well as their antagonistic ability to Penicilliumexpansum and Alternaria alternata in vitro when applied with fungicide.There were significant differences in sensitivity to the fungicides among the different yeasts(P = 0.05).R.glutinis was more sensitive to Deccocil,Iprodione and Stroby as compared to other yeasts.Combination antagonistic yeasts with fungicide could more significantly enhance biocontrol ability of the yeasts against the pathogenic fungi in vitro(P = 0.05).C.laurentii was the most effective antagonist among the four yeasts and could completely control spore germination of P.expansum and A.alternata when combined with Stroby at the concentration of 100 μL L-1.The yeasts,except R.glutinis,could grow well on nutrient yeast dextrose agar (NYDA) after 8 d incubation even at 20% CO2 concentration at 25℃.Particularly Trichosporon sp.showeda better adaptability to low temperature as compared to other antagonists.     

甘薯新品种综合评价初探

对福建省新育成甘薯品种应用灰色加权关联度分析法进行综合评估，旨在鉴定新品种在丘陵旱地的适应性以及探讨出一种比较适宜的品种评估方法。结果表明，龙薯1号、莆薯41和泉薯647比较适应东南沿海丘陵旱地生产条件，评估结果与品种的实际表现比较一致。     

细胞因子对动物免疫与物质代谢的影响

细胞因子种类很多 ,本文主要就几类细胞因子———白细胞介素 (IL)、肿瘤坏死因子 (TNF -α)和干扰素 (IFN -γ)与动物免疫的关系及细胞因子对物质代谢的调控方面进行综述。     

迪卡配套系猪RYR1的PCR分析及其利用的研究

试验从鸡东县永安猪场随机抽取迪卡配套系猪B系和E系42头，通过PCR扩增技术和酶切鉴定．采用克隆技术进行测序，来检测是否含有氟烷基因序列。经过以上技术检测这42头猪均没有氟烷基因。     

棉花冠层对不同灌水量的反应及其产量形成研究

通过探讨新疆北部棉区推广棉种新陆早6号与新陆早8号,在不同灌水量条件下冠层结构特征的差异性,得出新陆早6号对限量滴灌反应不敏感,而新陆早8号对限量滴灌反应较敏感。因此,在限量滴灌条件下,选择新陆早6号种植较适宜。叶面积指数低,叶片平均倾斜角度大,群体散射辐射透过系数和群体直射辐射透过系数增加,光能的截获率降低,由此得出,不同品种对灌水量的反应表现及理想群体冠层结构的合理指标,为增加棉花产量和提高水分利用效率提供了理论依据。     

5日龄雏鹅痛风的诊治

家禽痛风是由于其体内尿酸产生过多或排泄障碍,造成体内大量尿酸蓄积,并以尿酸盐的形式沉积在关节、内脏器官浆膜面及其它组织间质,从而引起患禽运动迟缓、排白色稀粪、厌食、衰竭,甚至导致死亡.     

影响吉林省亚洲玉米螟〔Ostrinia furnacalis（Guenee）〕一代发生区发生量长期预测的关键因子Ⅱ.发生程度与降雨及种群质量的关系

玉米螟的发生程度与5～8月初的降雨和种群质量密切相关，降雨主要影响越冬种群的复苏、化蛹、羽化、成虫的活动及3令前幼虫的存活，5～6月份特别干旱不利于越冬种群的复苏、化蛹和羽化．对成虫的产卵量有影响；7月份的干旱或大暴雨严重影响成虫的活动、卵的孵化和3令前幼虫的存活。不同年度间一、二化种群在越冬种群中所占的比例影响越冬种群的质量，在以一化种群为主时，玉米螟繁殖力强、发生晚，危害重。     

日粮锌、硒水平对肉鸡肠道黏膜屏障结构的影响

为了探讨微量元素锌和硒相互作用对肉鸡肠道黏膜屏障结构的影响，将24只1日龄AA肉鸡随机分3组，分别饲喂添加有高锌高硒(锌1000mg／kg、硒5mg／kg)、低锌低硒(锌34mg／kg、硒0．08mg／kg)或常锌常硒(锌50mg／kg、硒0．15mg／kg)的日粮45d后，观察肠黏膜上皮细胞、上皮内淋巴细胞和盲肠扁桃体的形态结构变化。结果表明：高锌高硒或低锌低硒组肉鸡的肠黏膜结构有明显的损伤，表现为肠黏膜上皮细胞萎缩，绒毛长度下降，上皮内淋巴细胞数量减少；盲肠扁桃体的弥散淋巴组织和淋巴小结中，淋巴细胞数量减少，细胞出现肿胀，有的核消失，结缔组织增生，淋巴小结萎缩。尤其是高锌高硒组的损伤最为严重。而常锌常硒组肉鸡肠黏膜和盲肠扁桃体的形态结构正常。结论：日粮中按锌50mg／kg、硒0．15mg／kg的比例添加，对于维持肠道黏膜的正常屏障结构是合适的。过高或过低的锌和硒对小肠黏膜有毒性作用，破坏其屏障功能；而且高锌和高硒可相互促进以增强其毒性作用。     

宁陕县城区生态环境治理工程初探

介绍并分析宁陕县城区及周边地区“8．29”惨遭洪水、泥石流袭击,生态环境遭到严重破坏的情况,提出了城区生态环境方面存在的问题,同时在规划设计方案中运用生态治理技术,以工程造林的方法,探求城区生态环境建设的新模式。