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111.
Oral administration of Escherichia coli lipopolysaccharide enhances the immune system of striped catfish,Pangasianodon hypophthalmus (Sauvage) 下载免费PDF全文
Lipopolysaccharide (LPS), a component of Gram negative bacteria, was reported as important immunostimulant for fish. In this study, striped catfish were fed diets containing different Escherichia coli LPS concentrations (0%, 0.01% and 0.05%) for 2 weeks and then fed control feed (0% LPS) for 4 weeks. Plasma cortisol and glucose were rather low and did not differ significantly among treatments (P > 0.05). The respiratory burst activity, lysozyme, complement, total of antibody as well as mortality in fish challenged with Edwardsiella ictaluri were recorded every 2 weeks (W2, W4 and W6). The lysozyme activity significantly increased in fish treated with LPS (P < 0.05) in W2, W4 and W6. The highest values of respiratory burst activity were observed at week 4 in fish fed 0.01% LPS. There were significant differences in total of antibody between fish fed LPS (0.01%) and control in W2, W4. The challenge test with Edwardsiella ictaluri showed that fish fed 0.01% LPS had lower cumulative mortality (40%, 33% and 42%) compared with the fish fed 0.05% LPS (50%, 40% and 47%) and control fish (40%, 57% and 53%) in the three difference sampling times respectively. These results suggest that feed supplemented with 0.01% LPS could enhance immunity of striped catfish after 2 weeks of oral administration and fish could be protected against bacterial infection during the following 4 weeks. 相似文献
112.
Utilization of fermented soybean meal for fishmeal substitution in diets of Pacific white shrimp (Litopenaeus vannamei) 下载免费PDF全文
N. Van Nguyen L. Hoang T. Van Khanh P. Duy Hai L. T. Hung 《Aquaculture Nutrition》2018,24(3):1092-1100
Two feeding experiments were carried out to evaluate the utilization of fermented soybean meal (FSBM) in diets for Pacific white shrimp (Litopenaeus vannamei). The digestibility experiment was assessed with four diets: a reference diet and three ingredient test diets containing FSBM, local and Chilean fishmeal. The growth experiment was conducted including four isonitrogenous and isocaloric diets formulated to contain the graded levels of dietary fishmeal (0 g/kg, 150 g/kg, 300 g/kg and 450 g/kg) substituted by FSBM. The results indicated that ADCs of crude protein in FSBM were high (888.4 g/kg) and equal in local fishmeal but little lower than Chilean fishmeal while without any significant differences observed in ADCs of crude lipid among the different test ingredients. After 75 days of growth trial, survival rates and feed conversion ratio were not significantly different among the experimental treatments. However, shrimp fed the diets with increasing inclusions of FSBM had a tendency to reduce weight gain and specific growth rate. Based on the correlation between weight gain and substituted fishmeal level analysed by broken‐line regression, the optimum level of fishmeal replaced by FSBM in diet was 253.6 g/kg without adverse effects on growth and feed utilization of Pacific white shrimp. 相似文献
113.
Duong Tan Nhut Truong Thi Thuy AnNguyen Thi Dieu Huong Nguyen Trinh DonNguyen Thanh Hai Nguyen Quoc ThienNguyen Hong Vu 《Scientia Horticulturae》2007
An unique procedure for the mass shoot propagation of Gerbera using receptacle transverse thin cell layer (tTCL) culture procedure was developed. Genotype, flower bud age, explant size, position of receptacle tTCLs and culture media were found to affect the success of culture. Ten interspecific crosses of Gerbera showed different shoot regeneration rates and callus induction via receptacle tTCL culture, all of which had shoot regeneration rates higher than 57%. Flower buds collected on the 10th day resulted in 91% shoot regeneration after 6 weeks of culture on basal MS medium [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15, 475–497] supplemented with 0.02 mg l−1 thidiazuron (TDZ), 0.8 mg l−1 adenine and 10% (v/v) coconut water (CW). This was significantly higher than those from flower buds on the 7th and 14th days (22% and 54%), respectively. Shoot regeneration rate was the highest (94–100%) in the middle layers of the receptacle. For mass shoot propagation, shoot clusters were subcultured on half-strength MS medium supplemented with 0.5 mg l−1 indole-3-butyric acid (IBA), 0.5 mg l−1 6-benzyladenine (BA) and 2.0 mg l−1 kinetin after every 4 weeks. Plantlets formed when single shoots were cultured on half-strength MS medium containing 1 mg l−1 IBA. All plantlets acclimatized well in the greenhouse. 相似文献
114.
Nguyen Phuc Cam Tu Nguyen Ngoc Ha Tetsuro Agusa Tokutaka Ikemoto Bui Cach Tuyen Shinsuke Tanabe Ichiro Takeuchi 《Fisheries Science》2010,76(4):677-686
The dominant coastal bivalve in Vietnam, hard clams Meretrix spp., collected from the South Key Economic Zone (SKEZ), the Mekong River Delta, and the Central Coastal Zone (CCZ) were
analyzed for 21 trace elements. Comparison of the results from the three regions indicated that levels of most of the trace
elements, especially As, Mo, Sn, and Pb, were highest in the samples collected from the CCZ, whereas most of the trace elements
were found to be present at relatively low levels in samples from the SKEZ. The high concentrations of these trace elements
in the CCZ, a sparsely populated region with less human activity than the other two regions, were believed to have originated
from industrial waste produced in a shipyard. Although the trace element concentrations in the bivalves were within safe levels
for human-consumption criteria reported by the United State Food and Drug Administration and the European Commission, estimation
of cancer risk based on As concentration indicates that the hard clams from the CCZ pose a high risk to consumers. Thus, the
industrial waste produced in the less densely populated region might increase the health risk to consumers via the contamination
of bivalves commonly used as food. 相似文献
115.
Nguyen Thi Ngoc Anh Mathieu Wille Nguyen Van Hoa Patrick Sorgeloos 《Journal Of Applied Aquaculture》2013,25(3):256-270
Supplementation of microalgae and Artemia nauplii with practical formulated feeds containing fresh or dried Artemia biomass for larval rearing of black tiger shrimp, Penaeus monodon, was assessed. Five feeding treatments were carried out in a recirculating seawater system with fifteen 30-L fiberglass tanks. Shrimp nauplii were stocked at a density of 150 L?1 for 23 days. In the control treatment, live feed was supplemented with commercial formulated feed (Inve Aquaculture NV, Belgium). In two other treatments, live feed was supplemented with a pelleted feed based on either fresh or dried Artemia. In the remaining two treatments live feed was supplemented with a combination of 50% commercial feed and 50% fresh or dried Artemia feeds. Overall, performance of PL in the combination treatments (commercial feed and Artemia diets) were equal to or better than those fed commercial feed alone as seen by the better growth rate and higher resistance to formalin stress. The results indicate that feed containing fresh or dried Artemia biomass can partially supplement live feeds for larval rearing of P. monodon. 相似文献
116.
Summary The Giemsa banding patterns of four inbred lines (DB1113, DB1116, WIR44 and WIR27) ofZea mays and their hybrids have been compared. The polymorphism of knob heterochromatin of 4 chromosome pairs allowed to distinguish the 4 inbred lines on the basis of their banding patterns, reflected the evolutionary distance of the inbred lines and provided evidence as to the origin of these chromosomes in hybrids.Sister chromatid differentiation by means of BrdUrd incorporation prior to fluorescence plus Giemsa(FPG)-technique revealed 3.7 SCEs per cell for the hybrid BEKOS 251. Individualization of chromosomes on the basis of their replication patterns or asymmetric bands after application of the same technique was impossible.
Differentialfärbung mitotischer Chromosomen von Inzuchtlinien und deren Hybriden beiZea mays L.
Zusammenfassung Die Giemsabandenmuster der vier Inzuchtlinien des Maises DB1113, DB1116 (convar.vulgaris) und WIR27, WIR44 (convar.dentiformis) sowie der daraus entstandenen Hybriden SU 10, Kappa und BEKOS 251 wurden untereinander verglichen. Polymorphismen des Knob-Heterochromatins auf vier Chromosomenpaaren (I, VI, VII, VIII) erlaubten die Unterscheidung der vier Inzuchtlinien anhand ihrer Bandenmuster, widerspiegelten den jeweiligen Verwandtschaftsgrad zwischen den Inzuchtlinien und ermöglichten für einige Chromosomen der Hybriden den Herkunftsnachweis.Schwesterchromatidendifferenzierung nach BrdUrd-Einbau und FPG-Färbung ergaben für die Doppelhybride BEKOS 251 3.7 SCEs/Zelle. Die Erfassung von Replikationsmustern oder asymmetrischen Banden zur Charakterisierung individueller Chromosomen war mit dieser Methode nicht möglich.
Zea mays L.
, , DB1113, DB1116 (convar.vulgaris) WIR27, WIR44 (convar.dentiformis), SU 10, Kappa BEKOS 251. - (I, VI, VII, VIII) , . BrdUrd FPG BEKOS 251 3,7 . .相似文献
117.
Lacroux C Nguyen TH Andreoletti O Prevot F Grisez C Bergeaud JP Gruner L Brunel JC Francois D Dorchies P Jacquiet P 《Veterinary research》2006,37(4):607-622
Selection of resistant animals and host immunization have been proposed as alternative methods for the control of gastrointestinal nematode parasites. However, a better knowledge of the mechanisms involved in protective immunity against these parasites is required for the development of optimal strategies. In this study, 3 month old INRA 401 lambs (n = 81) were allocated into three groups: uninfected control, challenged either once (primary-infected animals) or twice (previously-infected animals) with 10,000 Haemonchus contortus L3. Uninfected control and challenged animals were sequentially sacrificed at 3, 7, 15 and 28 days post challenge. In both challenged groups, a clear Th2-oriented immune response was observed in the abomasal lymph node and mucosa. IL-4 and IL-13 mRNA over-expression, recruitment of eosinophils, mast cells and globule leukocytes and production of specific systemic IgG and mucosal IgA were observed earlier in previously-infected animals than in primary-infected ones. At 28 days post infection, no differences between intensities of these responses were observed between the challenged groups. Worm establishment rates were similar in previously-infected and primary-infected lambs. However, reductions of worm development, female fecundity and fecal egg output were observed in previously-infected sheep. We conclude that H. contortus infection in young INRA 401 lambs induced an unequivocal Th2 immune response resulting in the regulation of worm egg production without affecting their establishment. 相似文献
118.
Kawabata A Yamamoto K Lan NT Uchida K Yamaguchi R Hayashi T Tateyama S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(10):1047-1053
A cell line, MCO-Y4, was established from a mammary gland osteosarcoma of a 16-year-old female mongrel dog. Histopathologically the tumor was composed of osteoblastic cells with an osteoid meshwork and chondroid matrix. The mean doubling time of the cells at the 93rd passage was 32.39+/-4.66 hr. Immunohistochemically, the osteoblastic and chondroblastic cells were positive for bone morphogenetic protein (BMP)-2/4 and BMP receptor (BMPR) II. The cultured cells were spindle in shape during the growth and the confluent phases. No tumor matrix was detected in the culture dish by alcian blue staining or von-Kossa silver impregnation. MCO-Y4 cells on the chamber slides showed intense immunoreactivity for BMP-2/4 and BMPR II. Noggin, an antagonist for BMP-2/4, showed the growth inhibition on MCO-Y4 cells. In addition, fibronectin might be potential for stimulating growth of MCO-Y4 cells. When transplanted into severe combined immunodeficiency mice, the cells formed tumors consisting of solid proliferation of osteoblastic and fibroblastic cells with woven-bone trabeculae. These tumor cells were intensely positive for BMP-2/4 and BMPR II. Our results suggested that the cell line might be useful for studying the role of BMPs in canine osteosarcoma and the mechanism of ossification. 相似文献
119.
Dong Khuu Thi Phuong Duc Nguyen Minh Hoa Nguyen Thi Ngoc Hanh Bui Le Thai Trang Le Thi Thu 《Aquaculture International》2021,29(3):1265-1277
Aquaculture International - Food incidents have led to the increase in demand for food products certified by an international quality assurance certification of consumers all over the world. Shrimp... 相似文献
120.
Chérif N Thiéry R Castric J Biacchesi S Brémont M Thabti F Limem L Hammami S 《Veterinary research communications》2009,33(4):345-353
Viruses belonging to the Nodaviridae family cause disease worldwide among a large number of species of marine fish, and have
been described in all continents. In the present study, a total of 69 farmed Tunisian sea bass (Dicentrarchus labrax) and
24 sea bream (Sparus aurata) samples were tested monthly for the detection of betanodavirus. The virus was identified in both
species using indirect immunofluorescence assays (IFAT) and RT-PCR. In addition sequence analysis of part of the coat protein
gene indicated that both species were infected by highly related, but distinct, strains belonging to the RGNNV genotype. The
sequence of the coat protein gene of several strains was identical but up to 9 different sequences were detected in a single
farm. In addition, viral sequences obtained from fish that were held at lower temperature (<20°C) were distinct from the rest
of the sequences. 相似文献