森林可持续经营的社会福利效应分析

森林具有较强的正外部性,森林的可持续经营对保持和维护森林的外部性具有很强的作用.分析了森林可持续经营的意义及世界主要国家关于森林可持续经营方面的立法,研究人工林和天然林在自给自足和自由贸易情况下实施可持续经营的社会福利效应,并对我国森林实施可持续经营进行了展望.     

SALTIRSOIL: a simulation model for the mid to long‐term prediction of soil salinity in irrigated agriculture

The SALTIRSOIL model predicts soil salinity, sodicity and alkalinity in irrigated land using basic information on soil, climate, crop, irrigation management and water quality. It extends the concept of the WATSUIT model to include irrigation and crop management practices, advances in the calculation of evapotranspiration and new algorithms for the water stress coefficient and calculation of electrical conductivity. SALTIRSOIL calculates the soil water balance and soil solution concentration over the year. A second module, SALSOLCHEM, calculates the inorganic ion composition of the soil solution at equilibrium with soil calcite and gypsum at the soil’s CO₂ partial pressure. Results from comparing predicted and experimentally determined concentrations, observations and predictions of pH, alkalinity and calcium concentration in calcite‐saturated solutions agree to the second significant figure; in gypsum‐saturated solutions the standard difference between observations and predictions is <3% in absolute values. The algorithms in SALTIRSOIL have been verified and SALSOLCHEM validated for the reliable calculation of soil salinity, sodicity and alkalinity at water saturation in well‐drained irrigated lands. In simulations for horticultural crops in southeast Spain, soil solution concentration factors at water saturation, quotients of electrical conductivity (EC₂₅) at saturation to electrical conductivity in the irrigation water, and quotients of sodium adsorption ratio (SAR) are very similar to average measured values for the area.     

Mild Drought Stress‐Induced Changes in Yield,Physiological Processes and Chemical Composition in Festuca,Lolium and Festulolium

The impact of mild drought stress (3 weeks at 40 % field water capacity) on yield, physiological processes, accumulation of proline and phenolic compounds and forage quality parameters in forage grasses was evaluated in pot experiments. During four different growing periods, the effects of water deficit were assessed in nine varieties from five species (Lolium perenne, Lolium multiflorum, Festuca pratensis, Festuca arundinacea and Festulolium braunii). All measured parameters were affected by drought stress in the different cuts. Photosynthesis, transpiration rate, stomatal conductance and dry matter yield were significantly lower in drought stress than under well‐watered conditions in all varieties. Higher water‐use efficiency was only observed during the first and fourth drought period, while this was not the case in the second and third. Mild drought stress significantly increased the content of proline, phenolic acid, flavonoids, water‐soluble carbohydrates and protein. All tested grasses showed also an increase of organic matter digestibility and cell wall digestibility under drought stress conditions.     

朱砂根皂苷高产愈伤组织的诱导与筛选

采用比色法测定了以朱砂根(Ardisia crenata)胚、根、茎及叶等4种外植体诱导的愈伤组织中皂苷含量,筛选高皂苷含量的愈伤组织。结果表明,朱砂根的胚和根诱导出来的愈伤组织皂苷含量最高,达到24.07 mg/g和21.25 mg/g,茎和叶诱导的愈伤组织皂苷含量较低,根和茎诱导的愈伤组织生长较好。     

Dietary fiber in a low-protein diet during gestation affects nitrogen excretion in primiparous gilts,with possible influences from the gut microbiota

We investigated the effects of dietary fiber (DF) supplementation in normal or low crude protein (CP) diets on reproductive performance and nitrogen (N) utilization in primiparous gilts. In total, 77 Landrace × Yorkshire pregnant gilts were randomly allocated to four dietary treatments in a 2 × 2 factorial design. The groups comprised 1) equal intake of normal CP (12.82% and 0.61% total lysine), 2) low CP (LP) (10.53% and 0.61% total lysine), and 3) with or 4) without DF supplementation (cellulose, inulin, and pectin in a 34:10:1 ratio). A low-protein diet during gestation significantly reduced daily weight gain from days 91 to 110 of pregnancy (−162.5 g/d, P = 0.004). From N balance trials conducted at days 35 to 38, 65 to 68, and 95 to 98 of pregnancy, DF addition increased fecal N excretion at days 65 to 68 (+24.1%) and 95 to 98 (+13.8%) of pregnancy (P < 0.05) but reduced urinary N excretion (P < 0.05), resulting in greater N retention at each gestational stage. DF increased fecal microbial protein levels and excretion during gestation. An LP diet also reduced urinary N excretion at different gestational stages. An in vitro fermentation trial on culture media with nonprotein N urea and ammonium bicarbonate (NH₄HCO₃) as the only N sources revealed that microbiota derived from feces of gestating gilts fed the high DF diet exhibited a greater capacity to convert nonprotein N to microbial protein. Microbial fecal diversity, as measured by 16S rRNA sequencing, revealed significant changes from DF but not CP diets. Gilts fed an LP diet had a higher number of stillbirths (+0.83 per litter, P = 0.046) and a lower piglet birth weight (1.52 vs. 1.37 kg, P = 0.006), regardless of DF levels. Collectively, DF supplementation to gestation diets shifted N excretion from urine to feces in the form of microbial protein, suggesting that the microbiota had a putative role in controlling N utilization from DF. Additionally, a low-protein diet during gestation negatively affected the litter performance of gilts.     

过表达MyBPC1对牛骨骼肌卫星细胞增殖分化的影响

旨在探究肌球蛋白结合蛋白C1（myosin binding protein C1,MyBPC1）对牛骨骼肌卫星细胞增殖与成肌分化的影响,为进一步研究MyBPC1在细胞分化和肌肉发育过程中的调控作用提供依据。本研究利用西门塔尔胎牛原代牛骨骼肌卫星细胞体外诱导成肌分化模型模拟牛骨骼肌的生长发育过程。采用qRT-PCR和Western blot检测MyBPC1的细胞时序表达谱。试验分为两组。在RNA水平每组4个重复,每个重复20 μL;在蛋白水平每组3个重复,每个重复15 μg。采用qRT-PCR和Western blot检测牛骨骼肌卫星细胞转染MyBPC1的过表达效果,并进一步检测细胞增殖期标志因子Pax7、Ki67以及细胞分化期标志因子MyHC、MyOG的表达变化情况,观察牛骨骼肌卫星细胞肌管形成状态。结果,MyBPC1在牛骨骼肌卫星细胞分化前后表达水平存在极显著差异,牛骨骼肌卫星细胞诱导分化后MyBPC1的mRNA和蛋白表达量均极显著高于增殖期（P<0.01）。过表达MyBPC1后,细胞分化形成的肌管数量明显多于对照组,增殖标志因子Pax7的mRNA水平和蛋白表达水平无显著差异,分化标志因子MyHC的mRNA水平和蛋白表达水平极显著高于对照组（P<0.01）。过表达MyBPC1可以促进牛骨骼肌卫星细胞体外成肌分化,为进一步开展MyBPC1对牛骨骼肌卫星细胞的调控机制奠定基础。     

Chitosan nanoparticles enhance developmental competence of in vitro-matured porcine oocytes

Oxidative stress is inevitable as it is derived from the handling, culturing, inherent metabolic activities and medium supplementation of embryos. This study was performed to investigate the protective effect of chitosan nanoparticles (CNPs) on oxidative damage in porcine oocytes. For this purpose, cumulus–oocyte complexes (COCs) derived from porcine slaughterhouse ovaries were exposed to different concentrations of CNPs (0, 10, 25 and 50 µg/ml) during in vitro maturation (IVM). Oocytes treated with 25 µg/ml CNPs showed significantly higher levels of GSH, along with a significant reduction in ROS levels compared to control, CNPs10 and CNPs50 groups. In parthenogenetic embryo production, the maturation rate was significantly higher in the CNPs25 group than that in the control and all other treated groups. In addition, when compared to the CNPs50 and control groups, CNPs25-treated oocytes showed significantly higher cleavage and blastocyst development rates. The highest concentration of CNPs reduced the total cell number and ratio of ICM: TE cells in parthenogenetic embryos, suggesting that there is a threshold where benefits are lost if exceeded. In cloned embryos, the CNPs25 group, as compared to all other treated groups, showed significantly higher maturation and cleavage rates. Furthermore, the blastocyst development rate in the CNPs25-treated group was significantly higher than that in the CNPs50-treated group, as was the total cell number. Moreover, we found that cloned embryos derived from the CNPs25-treated group showed significantly higher expression levels of Pou5f1, Dppa2, and Ndp52il genes, compared with those of the control and other treated groups. Our results demonstrated that 25 µg/ml CNPs treatment during IVM improves the developmental competence of porcine oocytes by reducing oxidative stress.     

日粮蛋白质水平对云南半细毛羊空怀母羊能量代谢的影响

为研究日粮蛋白质水平对云南半细毛羊空怀母羊能量代谢的影响,试验选择25只云南半细毛羊空怀母羊,平均分为5个组,分别饲喂蛋白质水平为8.4%、10.0%、12.3%、13.6%、15.9%的日粮,用全收粪法进行消化代谢试验,试验期共19 d,预试期14 d,正试期5 d。结果表明：（1）日粮蛋白质水平对总能摄入量无显著影响（P > 0.05）。（2）从组1到组5粪能逐渐下降,且组3、组4、组5较组1分别降低了10.5%、11.4%、13.7%（P < 0.05）,组5较组2降低了9.5%（P < 0.05）。（3）从组1到组5尿能逐渐增加,除组2与组1、组3间差异不显著（P > 0.05）外,其余组间差异极显著（P < 0.01）。（4）组4和组5总能消化率较组1极显著提高13.2%和11.8%（P < 0.01）,其余各组差异不显著（P > 0.05）。组4总能代谢率较组1极显著提高9.8%（P < 0.01）,其余各组差异不显著（P > 0.05）。对于消化能代谢率,组1和组2差异不显著（P > 0.05）,组3、组4和组5依次显著降低（P < 0.05）。（5）蛋白质采食量与总能消化率、消化能代谢率间的回归方程分别为：Y=0.917X+45.872（R2=0.853）|Y=-0.370X+87.735（R2=0.899）。综上,日粮蛋白质水平与粪能呈负相关,与尿能呈正相关。日粮蛋白质水平为13.6%时,总能消化率和总能代谢率均较8.4%蛋白质水平组极显著提高。总能消化率随蛋白质采食量的提高而线性升高,消化能代谢率则随蛋白质采食量的提高而线性降低。 [关键词] 蛋白质|云南半细毛羊|空怀期|能量代谢