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91.
Antoine Decamp Orane Michelo Christelle Rabbat Cline Laroche Dominique Grizeau Jrmy Pruvost Olivier Gonalves 《Marine drugs》2021,19(2)
In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures. The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides’ (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system. Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization. Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis. Initial results showed that the enzymes were not sensitive to the presence of TFA and NaOH, so the methodology could be carried out on fresh hydrolysate. The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance. A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol. Finally, analyses of raw culture media were carried out and compared to the results obtained in miliQ water; no differences were observed. The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors. 相似文献
92.
Dominique SERRANI Ilario FERROCINO Cristiana GAROFALO Andrea OSIMANI Maria Rita CORVAGLIA Vesna MILANOVI Lucia AQUILANTI Stefania COCCO Valeria CARDELLI Rogrio Borguete Alves RAFAEL Elena FRANCIOSI Kieran TUOHY Francesca CLEMENTI Giuseppe CORTI 《土壤圈》2023,33(3):508-520
The slash-and-burn system is a subsistence agronomical practice widespread in tropical areas worldwide. This system has been extensively studied,especially for its impacts on agronomical aspects and soil physicochemical properties; however, knowledge of soil microbial diversity under slash and bum is scarce. In this study, for the first time, soil bacterial diversity of three locations from Central Mozambique, where slash and burn has been practiced for different durations of the forest fallow p... 相似文献
93.
Christopher Foote Chris T. Darimont Michel Baguette Simon Blanchet Luke M. Jacobus Dominique Mazzi Josef Settele 《BMC ecology》2017,17(1):28
For the fifth year, BMC Ecology is proud to present the winning images from our annual image competition. The 2017 edition received entries by talented shutterbug-ecologists from across the world, showcasing research that is increasing our understanding of ecosystems worldwide and the beauty and diversity of life on our planet. In this editorial we showcase the winning images, as chosen by our Editorial Board and guest judge Chris Darimont, as well as our selection of highly commended images. Enjoy! 相似文献
94.
Boulanger Yan Arseneault Dominique Boucher Yan Gauthier Sylvie Cyr Dominic Taylor Anthony R. Price David T. Dupuis Sébastien 《Landscape Ecology》2019,34(1):159-174
Landscape Ecology - Forest landscapes at the boreal–temperate ecotone have been extensively altered. Reducing the gap between current and presettlement forest conditions through... 相似文献
95.
Boucher Dominique Gauthier Sylvie Thiffault Nelson Marchand William Girardin Martin Urli Morgane 《New Forests》2020,51(4):543-571
New Forests - Climate change is projected to increase fire severity and frequency in the boreal forest, but it could also directly affect post-fire recruitment processes by impacting seed... 相似文献
96.
Jaime Aguayo Cline Fourrier‐Jeandel Xavier Capdevielle Fabrice Vtillard Dominique Piou Eugenia Iturritxa Ccile Robin 《Forest Pathology》2020,50(1)
Fusarium circinatum is the causal agent of pitch canker, a destructive disease that threatens natural and planted pine forests around the world. Although pitch canker has caused problems in Spain and Portugal, concerning Europe as a whole, the fungus is not established across the pine distribution area. Its dispersion by wind and/or insect vectors could nevertheless play a role in the colonization of currently uninfected stands. It is therefore crucial to develop monitoring tools for its detection. To this end, we assessed the molecular detection of the pathogen in environmental samples of bark beetles and passive spore traps, collected in two infected Pinus radiata plantations in Basque country, Spain. The spread pattern of F. circinatum was assessed by an experimental design that included insect and spore traps installed at the centre, at the edge and outside the plots. Our results showed that F. circinatum was detected in both types of samples, at almost all collection dates. In both type of samples, positive detections were mainly found at the centre of the plots, a lower proportion at the edge, and very few outside. This suggests that long‐distance dispersion of Fusarium circinatum does not rely on wind spore dispersal neither on insect flight. Our study also shows that molecular methods are a powerful tool to monitor the pathogen in environmental samples. 相似文献
97.
Logging pattern and landscape changes over the last century at the boreal and deciduous forest transition in Eastern Canada 总被引:4,自引:0,他引:4
Forestry practices associated with the industrial era (since ~1900) have altered the natural disturbance regimes and greatly
impacted the world’s forests. We quantified twentieth century logging patterns and regional scale consequences in three sub-boreal
forest landscapes of Eastern Canada (117,000, 49,400 and 92,300 ha), comparing forestry maps depicting age and forest cover
types for early industrial (1930) and present-day (2000) conditions. Results were similar for the three landscapes, indicating
large-scale forest change during the twentieth century. In 1930, previous logging activities had been concentrated in the
lowlands and along the main hydrographical network, as compared to a more even distribution over the landscapes in 2000, reflecting
a decreasing influence of the environmental constraints on forest harvesting. In 1930, old-aged forests (>100 years) accounted
for more than 75% of the unlogged areas of the three landscapes, as compared to less than 15% for the present-day conditions.
Logging practices have thus inverted the stand age distribution of the landscapes that are currently dominated by young and
regenerating stands. The 1930 forest cover types showed a clear relationship with elevation, with conifers located in the
lowlands and mixed and deciduous stands restricted to the upper slopes. Between 1930 and 2000, 58–64% of the conifer areas
transformed to mixed and deciduous forests, such that no clear altitudinal relationships remained in 2000. We conclude that
twentieth century logging practices have strongly altered the preindustrial vegetation patterns in our study area, to the
point that ecosystem-based management strategies should be developed to restore conifer dominance, altitudinal gradients,
as well as the irregular structure inspired from old forest stands. 相似文献
98.
This investigation compared the effects of using two binders of no nutritional value, lignosol and agar, and the binding method,
microbinding and microcoating, on the growth of juvenile Atlantic ditch shrimp, Palaemonetes varians, and rockpool prawn, Palaemon elegans. Shrimp were fed one basal diet which varied solely in binder type and method of inclusion (Diet A, with lignosol added by
microbinding, Diet B, with agar added by microbinding, and Diet C with lignosol added by microcoating) for a period of 45 days.
At the end of the experiment significant differences in the final wet weight were found between shrimps fed Diet A and Diet
C and between shrimps fed Diet B and Diet C (P < 0.001), but no significant differences were found between Diet A and Diet B (P > 0.05). These results were verified for both species. Significant differences were found between the linear regression slopes
of the three tested diets for P. varians (F
3,411 = 3.41; P < 0.04), and for P. elegans (F
3,431 = 10.51; P < 0.0003). Palaemon elegans grew faster than P. varians and at the end of the experiment significant differences were found between the final wet weight of the two species (F
3,842 = 48.17, P < 0.0001). The growth parameters analysed, weight gain (WG), thermal-unit growth coefficient (TGC), and food conversion rate
(FCR) were always higher for P. elegans. Results indicate that use of either lignosol or agar, added to the diet by microbinding, is advisable. 相似文献
99.
Michel Pépin Philippe Dufour Maurice Lambert Michel Aubert Aurèle Valognes Thierry Rotis Anne Van de Wiele Dominique Bergonier 《Journal of veterinary diagnostic investigation》2003,15(3):281-285
Serologic diagnosis of ovine contagious agalactia (Mycoplasma agalactiae) with the enzyme-linked immunosorbent assay (ELISA) developed by Agence Fran?aise de Sécurité Sanitaire des Aliments (AFSSA) may produce a few false-positive (FP) and false-negative (FN) results. When the prevalence of disease is low, these erroneous results may generate problems for eradication schemes. To prevent this, 2 commercial ELISAs were compared with the AFSSA ELISA. Flocks of known status were selected and classified into 4 categories: true positive (TP), FP, true negative (TN), and FN; 20 sheep per flock were submitted for blood sampling. A flock was considered positive when at least 1 out of 20 sera was positive or 2 sera were doubtful. In the flock, the diagnostic sensitivity of the 3 kits was very good (100%), and the diagnostic specificity showed an improvement from 46% (AFSSA test) to 88% and 92% (commercial tests). Considering individual animals, very few positive ewes were detected within TN or FP flocks; the proportion of positive ewes varied greatly from one kit to another (48% to 82%) within TP flocks. The kinetics of antibody response in sheep experimentally infected with various field strains of M. agalactiae were quite similar with all 3 ELISAs. The agreement between the 3 tests, assessed using the kappa value, varied from moderate to good (respective values of 0.56, 0.61, and 0.86). The 2 commercial ELISAs showed better performances, probably because of a superior analytical sensitivity, and are a good alternative for the serodiagnosis of contagious agalactia in sheep. 相似文献
100.