Humus balances of different farm production systems in main production areas in Austria

The importance of the soil humus content is indisputable. Soil humus plays an important role in preserving soil fertility and exerts great influence on plant production and yield potential. However, proofing that management‐related changes in the stock of soil organic matter (SOM) have taken place against the background of spatial and temporal variation is a difficult task. In most cases, sampling over a long period of time is needed to verify these changes. Alternatively, potential changes in the SOM stock can be estimated using humus balancing models, which help to identify the need for humus reproduction on a farm. In general, a humus balance is the difference between the humus demand of cultivated crops and humus supply through crops and organic fertilizers. In this study, the ‘Dynamic Humus Unit Method' within the modelling program REPRO was applied to calculate the humus balance for 29 model‐farms that are representative of most of the agricultural production in Austria. Each model‐farm represents a specific production type (PT) and farming system in a defined region or main production area (MPA). This approach gives an overview of the humus balances at a large scale and allows a general trend in Austria to be estimated. Besides differing between conventional and organic farming systems, specific site conditions can also be selected in the model. The constructed model‐farms belong to different PTs such as “forage production”, “cash crops”, “refinement”, and “permanent crops”. The PT “permanent crops” refers to the cultivation of wine. The cropping system of each PT was analyzed in detail, while livestock keeping was considered only when applicable. Positive humus balances were found for all PTs except for permanent crops. The results ranged from –122 to 890 kg C ha^?1 y^?1. Regions and farm structure, e.g., forage production compared to cash crop, were found to have a greater influence than the kind of farming system (i.e., organic vs. conventional farming). Comparing the different PTs, forage production had the highest positive humus balances (219 to 890 kg C ha^?1 y^?1), followed by cash crop (24 to 239 kg C ha^?1 y^?1), refinement (–64 to 402 kg C ha^?1 y^?1) and permanent crops (–122 to –38 kg C ha^?1 y^?1). Regarding the farming system, organic farming led to more humus accumulation than conventional farming due to a higher share of fodder legumes and catch crops and more diverse crop rotations. The results were within the range of available empirical data on SOM change, and it was therefore concluded that the results are reasonable. In general, humus reproduction can be regarded as sufficient for agricultural production.     

中国沿海两例食用织纹螺中毒事件中织纹螺体内毒素分析

近年来,因食用织纹螺(Nassariusspp.)导致的中毒事件在中国沿海屡有发生。由于中毒患者的症状与麻痹性贝毒中毒症状相似,因此,许多中毒事件被归咎于麻痹性贝毒,认为织纹螺中的毒素与邻近海域的有毒赤潮有关,但也有研究发现螺体内存在河豚毒素。对此,本研究应用亲水性相互作用色谱柱建立了河豚毒素的液相色谱-质谱联用分析方法,对造成2002年和2003年两次中毒事件的织纹螺样品进行分析。结果表明,两批织纹螺样品中均含有高浓度的河豚毒素及其衍生物,包括三脱氧河豚毒素、脱水河豚毒素和单加氧河豚毒素等,而且两个织纹螺样品中的毒素组成非常相似。因此,导致这两起中毒事件的致毒因子是河豚毒素及其衍生物。江苏和福建两地织纹螺中毒素组成的相似性显示两地织纹螺可能具有相同或相近的毒素来源。     

Pharmacological studies in an herbal drug combination of St. John's Wort (Hypericum perforatum) and passion flower (Passiflora incarnata): in vitro and in vivo evidence of synergy between Hypericum and Passiflora in antidepressant pharmacological models

Extracts of Hypericum, Passiflora and Valeriana are used for the treatment of mild depression and anxiety. We were interested whether a combination of Hypericum and Passiflora exerts comparable effects to Hypericum alone. We used two well-established models for investigating extracts for their anti-depressant activity, namely the effects on synaptic uptake of serotonin and the forced-swimming-test. We show here for the first time, that Passiflora significantly enhances the pharmacological potency of Hypericum in both models. Our data suggest that anti-depressive therapeutic effects of Hypericum are possible with lower doses, when it is combined with Passiflora, than with mono-preparations of Hypericum.     

Two-year dynamics of foliage labelling in 8-year-old <Emphasis Type="Italic">Pinus pinaster</Emphasis> trees with <Superscript>15</Superscript>N, <Superscript>26</Superscript>Mg and <Superscript>42</Superscript>Ca—simulation of Ca transport in xylem using an upscaling approach

• Introduction   

Atmospheric deposition is an important input of major nutrients into forest ecosystems. The long-term goal of this work was to apply stable isotope methodology to assess atmospheric nutrient deposition in forest systems.     

陕北黄土高原森林植被的性质和当前森林培育的策略

对比和分析了前人对陕北黄土高原植被的研究成果后认为陕北黄土高原的阔叶栎林为主的森林界限截止于渭北黄土高原北部,即陇县、彬县、宜君、铜川、黄龙一线。经过植被破坏和土壤侵蚀后土壤基质已经严重旱化和贫瘠化,通过重建植被土壤基质是可以逐步恢复的。植被的重建应该首选择先锋植物种类,造林后应及时进行改造,使其向接近该区天然林(植被)的方向发展,并需要建立一套可持续的森林经营体系。     

Fluctuations of element concentrations during the growing season of Polytrichum formosum (Hedw.)

Fourteen elements (Al, Ba, Ca, Cd, Cr, Cu, Fe, K, Mg, Ni, Pb, Sr, Ti, and Zn) were quantitatively determined by AES/ICP in aboveground parts of the endohydric moss Polytrichum formosum (Hedw.) after sampling over regular intervals from 1985 to 1987. With the exception of K, Mg, and Zn all elements showed a large continuous fluctuation (> 30%) during the growing season. Four different patterns of seasonal variation were found: (1) large seasonal variations of element concentrations (～80%) with maximum concentrations in winter and lowest concentrations in the summer months (Al, Fe, Cr, Mg, Pb, and Ti), (2) smaller seasonal variations (～50%) with maximum concentrations in winter and lowest concentrations in summer (Ba, Ca, Cd, Cu, Sr), (3) slight seasonal variations (～30%) with maximum concentrations in the summer (K), and (4) slight seasonal changes (～30%) with maximum concentrations in the winter (Mg and Zn). The relative biological variance of element concentrations between 9 different stands of Polytrichum formosum decreases in the following sequence: Ti, Al, Pb, Fe, Cr, Ni, Ba, Ca, Cu, Cd, K, Mg, Sr, Zn. The relative biological variance of individual elements is generally lower than the variance in element concentrations caused by seasonal changes. Because of its high biological variance for most elements investigated (seasonal as well as between different stands of Polytrichum formosum) this moss should only be used as a passive bioindicator, if all other environmental parameters are standardized.     

A Laboratory Study on Revegetation and Metal Uptake in Native Plant Species from Neutral Mine Tailings

Lygeum spartum, Zygophyllum fabago and Piptatherum miliaceum are typical plant species that grow in mine tailings in semiarid Mediterranean areas. The aim of this work was to investigate metal uptake of these species growing on neutral mine tailings under controlled conditions and their response to fertilizer additions. A neutral mine tailing (pH of soil solution of 7.1–7.2) with high total metal concentrations (9,100 and 5,200 mg kg^?1 Zn and Pb, respectively) from Southern Spain was used. Soluble Zn and Pb were low (0.5 and <0.1 mg l^?1, respectively) but the major cations and anions reached relatively high levels (e.g. 2,600 and 1,400 mg l^?1 Cl and Na). Fertilization caused a significant increase of the plant weight for the three species and decreased metal accumulation with the exception of Cd. Roots accumulated much higher metal concentrations for the three plants than shoots, except Cd in L. spartum. Shoot concentrations for the three plants were 3–14 mg kg^?1 Cd, 150–300 mg kg^?1 Zn, 4–11 mg kg^?1 Cu, and 1–10 mg kg^?1 As, and 6–110 mg kg^?1 Pb. The results indicate that neutral pH mine tailings present a suitable substrate for establishment of these native plants species and fertilizer favors this establishment. Metal accumulation in plants is relatively low despite high total soil concentrations.     

Structural profiling and quantification of sphingomyelin in human breast milk by HPLC-MS/MS

The sphingolipid composition of food as well as of physiological samples has received considerable interest due to their positive biological activities. This study quantified the total amount of sphingomyelin (SM) in 20 human breast milk samples from healthy volunteers and determined the structures of SM by detailed mass spectrometric studies in combination with enzymatic cleavage. The quantification of SM was performed by hydrophilic interaction liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (HILIC-HPLC-ESI-MS/MS) measuring the characteristic fragment ion of the phosphorylcholine group at m/z 184.2 and by using hexanoylsphingomyelin (C6-SM) and heptadecanoylsphingomyelin (C17-SM) as internal standards. The structures of SM species were identified after enzymatic cleavage with alkaline sphingomyelinase (SMase) to the corresponding ceramides. Structure elucidation of the sphingoid base and fatty acid backbone was performed by reversed-phase HPLC-ESI-MS/MS. The method includes the sphingoid bases dihydrosphingosine (d18:0), sphingosine (d18:1(Δ4)), 4,8-sphingadienine (d18:2(Δ4,8)), 4-hydroxysphinganine (phytosphingosine (t18:0)), and 4-hydroxy-8-sphingenine (t18:1(Δ8)) and fatty acids with even-numbered carbon atoms (C12-C26) as well as their (poly)unsaturated and monohydroxylated analogues. The total amount of SM in human breast milk varied from 3.87 to 9.07 mg/100 g fresh weight. Sphingosine (d18:1) was the predominant sphingoid base, with 83.6 ± 3.5% in human breast milk, followed by 4,8-sphingadienine (d18:2) (7.2 ± 1.9%) and 4-hydroxysphinganine (t18:0) (5.7 ± 0.7%). The main SM species contained sphingosine and palmitic acid (14.9 ± 2.2%), stearic acid (12.7 ± 1.5%), docosanoic acid (16.2 ± 3.6%), and tetracosenoic acid (15.0 ± 3.1%). Interestingly, the fatty acid composition of SM species in this study differs from the total fatty acids in human breast milk, and the fatty acids are not consistently distributed among the different sphingoid bases.     

Mycoplasma synoviae lipoprotein MSPB, the N-terminal part of VlhA haemagglutinin, induces secretion of nitric oxide, IL-6 and IL-1beta in chicken macrophages

Mycoplasma synoviae is a major pathogen of chickens and turkeys, causing respiratory disease and infectious synovitis. M. synoviae haemagglutinin VlhA is an abundant surface-exposed lipoprotein and immunodominant antigen. Post-translational cleavage of VlhA generates two proteins, MSPB and MSPA. MSPB, the amino-terminal end of VlhA, is a lipoprotein of about 40-50 kDa but can appear in truncated forms (tMSPB) of about 20-30 kDa. The aim of this study was to determine whether MSPB and tMSPB can stimulate chicken macrophages to secrete NO and cytokines. Macrophages derived from chicken monocytes (MDM) or the MQ-NCSU macrophage cell line were stimulated with M. synoviae protein extracts containing MSPB or tMSPB, as well as with purified MSPB and tMSPB. Proteins from detergent extractions induced IL-6 secretion in MDM and NO secretion in MQ-NCSU. Both MSPB and tMSPB were capable of inducing NO secretion in MQ-NCSU, as well as IL-6 and IL-1beta in MDM. The activity of IL-6 induced by purified tMSPB was similar to the effect of 60 pg/ml of recombinant chicken IL-6. The effect of IL-1beta induced by tMSPB was comparable to the effect of 10 ng/ml of recombinant IL-1beta. Whereas all samples containing MSPB were able to induce NO, IL-6 and IL-1beta, it seemed that the purified tMSPB of about 20 kDa was the most potent in its ability to induce IL-6 and IL-1beta in MDM. Compared to MSPB, tMSPB lacks about 200 amino acids in its carboxyl-terminal part. Therefore, our results suggest that the major part of the stimulating activity is associated with the amino-terminal part of MSPB, most likely with its lipid moiety.