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新农村建设是一项复杂的系统性工程,有无充足的资金支持决定了新农村建设的成败,该文分析了现有的主要筹资渠道的问题,提出了如何完善现有筹资渠道,广开源头,为新农村建设提供不竭的动力支持的建议。 相似文献
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Punnawich Yenjit Montree Issarakraisila Warin Intana Kan Chantrapromma 《Postharvest Biology and Technology》2010,55(2):129-132
Areca catechu L., a member of the Palmaceae family, is one of the most commonly used drugs in the world. Compounds obtained from the hexane, ethyl acetate and methanol extracts of the pericarp of A. catechu L. were assessed in vitro and in mango fruit for antifungal activity against Colletotrichum gloeosporioides Penz. In vitro studies also indicated that three triterpenes, namely fernenol (fern-9(11)-en-3α-ol), arundoin (fern-9(11)-en-3α-ol ME), and a mixture of stigmasterol and β-sitosterol, and one fatty acid, lauric acid, could inhibit the mycelial growth of C. gloeosporioides with EC50 values of 36.7, 47.5, 56.7 and 111.5 mg L?1, respectively. Furthermore, fernenol, arundoin, and the mixture of stigmasterol and β-sitosterol highly inhibited spore germination and germtube elongation. Mango fruit studies suggested that fernenol, arundoin and the mixture of stigmasterol and β-sitosterol were significantly more effective than benomyl for controlling postharvest anthracnose disease when used at 100 and 200 mg L?1. 相似文献
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SHI Dun-yun ZHANG Qiong-li LI Yu-zhu LI Ming XU Yun ZHUO Jia-cai DU Xin WANG Ming-chun 《园艺学报》2005,21(11):2264-2267
AIM: To explore the characters of fibroblast-like (F-L) cells cultured from granunocyte clony stimunating factor (G-CSF)-mobilized peripheral blood cell (PBC) harvests. METHODS: The adherent cells in the PBC harvests were cultured for 2 week in the mediums of RPMI-1640/L-DMEM/G-CSF or interleukin-3 (IL-3) plus RPMI-1640, the cultured F-L cells were analyzed by flow cytometry (FC). RESULTS: The adherent non-confluent F-L cells obtained from the four groups were similar in their phenotypes: CD33+, CD11c+, CD64+, CD14+, CD45+, HLA-DR+, CD86+, CD34-, CD38-, CD3-, CD19-, CD56-, CD29-, CD44-, CD105-. The F-L cells are similar to monocytes except CD38- and were distinct from dendritic cells (DC) or mesenchymal stem cells (MSC). CONCLUSION: The cultured F-L cells are macrophages rather than DC or MSC. G-CSF, rhIL-3 enhances their numbers. 相似文献
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采用变分方法考察了具有随机小费用的可修模型的维修策略问题,推导出了依赖年龄的最倨费用界维修策略所满足的微分方程及其边界条件。从而得到了费用界策略形式下的最优。对于包含预防性更换的可修模型也同样得到了费用界-预更换策略形式下的最优解。 相似文献
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Theo W. Prins Lia Wagemakers Jan A.L. van Kan 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(7):693-698
To identify genes of the necrotrophic pathogenic fungus Botrytis
cinerea that are expressed during infection of tomato leaves, a differential screening of a genomic library with radioactively labelled cDNA was performed. This resulted in the identification of a B. cinerea gene, denominated Bcubi4, which encodes a precursor protein consisting of four identical head-to-tail repeats of a 76aa ubiquitin unit. Subsequently a gene denominated Bcubi1CEP79, encoding a single ubiquitin unit joined to a Carboxyl Extension Protein of 79 amino acids, was isolated. The expression of the two ubiquitin genes was studied during pathogenesis of B. cinerea on tomato. Bcubi1CEP79, but not Bcubi4, mRNA was transiently induced at 16h after inoculation. The increased expression of the Bcubi1CEP79 gene at this stage of pathogenesis might be required for enhanced ribosomal biogenesis. 相似文献
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无菌采取5日龄SD大鼠的颅盖骨,用胰蛋白酶预消化后,漂洗,剪碎,再用Ⅱ型胶原酶消化获得成骨细胞。通过倒置相差显微镜观察,并用碱性磷酸酶和矿化结节染色鉴定,利用微量滴定(MTT)法测定一个培养周期的成骨细胞活性分布,探讨一种经济、高效的原代成骨细胞培养方法。倒置相差显微镜形态观察结果显示,未贴壁的细胞呈圆形,贴壁生长的细胞呈不规则梭形、三角形或多角形,单核,有1-3个核仁;碱性磷酸酶和钙化结节染色均呈阳性(阳性率≥98.06%);MTT法结合形态学观察结果显示,成骨细胞培养至第10 d时活性最强。证实用改良的二次酶消化法能够在短时间内获得大量成骨细胞,且所培养的细胞具有典型的成骨细胞形态和功能。 相似文献
29.
ZHU Hong-bo ZHUO Wen-ying HE Chao HUANG Xue-feng ZHU Yu-ping WANG Da FANG Bing-liang 《园艺学报》2007,23(2):231-235
AIM:To evaluate effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene and clarify the involved mechanisms in DLD1-TRAIL/R colon cancer cells. METHODS:Human colon cancer cell line DLD1-TRAIL/R cells that were resistant to TRAIL-expressing adenovector (Ad/gTRAIL) were treated with Ad/gTRAIL combined with different chemotherapeutic agents. Then, the cell viability was measured by MTT method, and apoptotic signaling conditions, including activation of caspase-3 and caspase-8, expression of Bax and Bcl-XL, were measured by Western blotting analysis. RESULTS:In vitro data showed that several chemotherapeutic agents, including 5-fluorouracil (5-FU) and mitomycin c (MMC), overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R colon cancer cells. The combination of Ad/gTRAIL and 5-FU effectively suppressed tumor growth in vivo in subcutaneous tumors established from DLD1-TRAIL/R cells. Further data showed that treatment with the combination of Ad/gTRAIL and 5-FU or MMC led to enhance the activation of caspase-3. Moreover, MMC but not 5-FU induced overexpression of Bax gene that was sufficient to overcome the resistance to TRAIL gene in DLD1-TRAIL/R cells. CONCLUSION:Chemotherapeutic agents, such as 5-FU and MMC, overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R cells. The candidate mechanisms for MMC but not 5-FU to overcome this resistance might involve the induction of over-expressed Bax protein in DLD1-TRAIL/R cells. 相似文献
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青海河湟地区水资源综合开发与区域经济发展研究 总被引:2,自引:0,他引:2
本文分析了河湟地区水资源特征、开发利用现状及存在的问题,在此基础上,论述了河湟地区水资源开发利用与区域经济发展模式。研究表明:加快开发黄河水能资源,建立以水能资源开采业为主导的产业群发展;浅山地区因地制宜推广雨水集流利用工程,走出“水窑+科技+节水产业+生态”的发展模式;“引大济湟”工程有助于提高水资源可利用量,推进湟水流域全面发展。 相似文献