瓣化型胡萝卜雄性不育基因atp6的CAPS标记建立

根据atp6的基因序列设计特异扩增引物,以瓣化型胡萝卜核质互作雄性不育系H05A及其保持系H05B为试材,扩增获得了1条约500bp的特异扩增片段。用限制性内切酶BglII对该片段进行酶切,不育系产生1条保持系中没有的特异片段,其分子量约为60bp。建立了atp6基因的CAPS标记,该分子标记可用于胡萝卜雄性不育分子标记辅助育种。     

干法保存30年的鲤鱼脑垂体催情异育银鲫产卵成功

首次报道用干法保存了30年的鲤鱼脑垂体催情异育银鲫产卵成功。当该垂体的剂量分别为10、20、30mg/kg亲鱼体重时,各获得50%、70%、90%的催产率,以及60.08±0.49%、70.42±0.32%、75.27±0.28%的受精率和79.93±0.29%、80.46±0.55%、81.10±0.26%的孵化率,平均每尾约250g体重的亲鱼产异育银鲫鱼苗0.976万尾。这些鱼苗和正常对照组的鱼苗一样,已经培育成体格健壮的优质鱼种。     

上海率先基本实现农业现代化的重要途径--"信息农业"研究

采用文献分析、实证研究和理论研究相结合的方法，探讨了上海发展信息农业与率先基本实现农业现代化的关系，分析了上海发展信息农业的基础条件和存在问题，提出了上海发展信息农业的决策建议，论述了建设上海农业信息系统的构思，可供有关部门参考。     

浅谈瞭望塔在林火监测体系中的应用

针对五常市林业局瞭望塔布局和选址情况，论述了瞭望塔建设应本着科学规划，合理布局，注重实效，善于管理的原则建设和应用管理。     

Role of IFNLR1 gene in PRRSV infection of PAM cells

BackgroundInterferon lambda receptor 1 (IFNLR1) is a type II cytokine receptor that clings to interleukins IL-28A, IL29B, and IL-29 referred to as type III IFNs (IFN-λs). IFN-λs act through the JAK-STAT signaling pathway to exert antiviral effects related to preventing and curing an infection. Although the immune function of IFN-λs in virus invasion has been described, the molecular mechanism of IFNLR1 in that process is unclear.ObjectivesThe purpose of this study was to elucidate the role of IFNLR1 in the pathogenesis and treatment of porcine reproductive and respiratory syndrome virus (PRRSV).MethodsThe effects of IFNLR1 on the proliferation of porcine alveolar macrophages (PAMs) during PRRSV infection were investigated using interference and overexpression methods.ResultsIn this study, the expressions of the IFNLR1 gene in the liver, large intestine, small intestine, kidney, and lung tissues of Dapulian pigs were significantly higher than those in Landrace pigs. It was determined that porcine IFNLR1 overexpression suppresses PRRSV replication. The qRT-PCR results revealed that overexpression of IFNLR1 upregulated antiviral and IFN-stimulated genes. IFNLR1 overexpression inhibits the proliferation of PAMs and upregulation of p-STAT1. By contrast, knockdown of IFNLR1 expression promotes PAMs proliferation. The G0/G1 phase proportion in IFNLR1-overexpressing cells increased, and the opposite change was observed in IFNLR1-underexpressing cells. After inhibition of the JAK/STAT signaling pathway, the G2/M phase proportion in the IFNLR1-overexpressing cells showed a significant increasing trend. In conclusion, overexpression of IFNLR1 induces activation of the JAK/STAT pathway, thereby inhibiting the proliferation of PAMs infected with PRRSV.ConclusionExpression of the IFNLR1 gene has an important regulatory role in PRRSV-infected PAMs, indicating it has potential as a molecular target in developing a new strategy for the treatment of PRRSV.     

高羊茅品种试验研究

１９９６年本所试验园进行了高羊茅品种特性对比试验，经过对供试草种的密度，均匀性，色泽，抗性等进行测定分析。结果表明；供试的６个品种，只要养护水平能跟上，在合肥地区均能保持四季常青。     

淳安县林业骨干苗圃建设的探讨

林业骨干苗圃的建设，是发展“一优二高”林业的物质基础，针对淳安县林业骨干苗圃的建设，科学地确定了建设的原则和规模，并对目前骨干苗圃的建设形式及存在问题提出了一些建议。     

1994年采自新疆的真菌鉴定报告

记述了１９９４年采自新疆布尔津，贾登玉，南山林场等地部分植物寄生真菌的３５个种。从采集的标本中初步鉴定出３５个种，其中白粉菌１６种，锈菌１３种，其他菌类６种。其中有５个种为新疆新记录，并对其进行了较详细的特征描述。