LED effect on free radical-scavenging activity of barley leaf

Barley leaf grown under light-emitting diode (LED) light was prepared and investigated for its free radical scavenging activity. LED light such as red, far-red, blue, blue-red, green, yellow, UVA, and white and also fluorescent light and dark conditions were also used in this study. Length and weight of barley leaves were measured and the Hunter color value, DPPH radical scavenging activity, and ABT radical-scavenging activity of barley leaf extracts were estimated. Extraction of barley leaf was performed by using a 70% ethanol solution. Length of barley leaf grown with red and green LED was 9.9 and 12.6 cm, respectively. Hunter L* and a* values of extracts of barley leaf grown under dark conditions were higher than those of other LED light-treated samples. The DPPH radical scavenging activity of extracts of barley leaf grown with blue, UVA, and under dark conditions were 41.40, 36.23, and 43.57%, respectively. ABT radical scavenging-activity of extracts of barley leaf grown under LED of red, far-red, blue, blue-red, green, yellow, and white were 5.46, 6.10, 6.00, 5.93, 5.74, 6.32, and 5.94 μmol TE g⁻¹, respectively.     

Enhanced seed production and metabolic alterations in Larix leptolepis by girdling

The demand for Japanese larch (Larix leptolepis) seeds has increased in Korea but their supply has been limited due to sporadic natural seed production. Among the various flower induction techniques, girdling woody stems has been recognized as a simple and efficient method to enhance seed production in a number of conifers. In this study, stem girdling was applied to 42-year-old Japanese larches, resulting in remarkable enhancement of seed cone production with no morphological changes in the seed characteristics. Metabolic alterations in needles of the girdled and the control trees were interrogated by using 23 individual metabolites including soluble sugars, polyamines and amino acids. In the girdled trees, the contents of both soluble sugars and polyamines were higher from June to July and then became similar to those in the control. The levels of amino acids were significantly increased only in August by girdling compared to the non-girdled trees. In control larch trees, the dominant amino acids were changed from Glu and Ala until July to Asp and Tyr in August. Interestingly, the concentrations of Asp-derived amino acids such as Lys, Met and Thr, were increased by girdling and they also showed significant correlations with the number of seed cones per trees, suggesting that the enhanced levels of three amino acids might positively influence on the development of seed cones in girdled larch trees. Although the role of Asp-derived amino acids on enhanced seed cone production remains unclear, the approach presented in this study might provide useful information in elucidating metabolic network modulation induced by girdling and will be further applied for enhanced seed cone production in Japanese larch trees.     

Natural Gradient Drift Tests for Assessing the Feasibility of In Situ Aerobic Cometabolism of Trichloroethylene and Evaluating the Microbial Community Change

The objective of this study is to develop a method for using the single-well natural gradient drift test (SWNGDT) in the field to assess in situ aerobic cometabolism of trichloroethylene (TCE) and to analyze microbial community changes. The SWNGDT was performed in a monitoring well installed in a TCE-contaminated aquifer in Wonju, South Korea. The natural gradient drift biostimulation test (NGDBT) and surrogate test (NGDST) were performed by injecting dissolved solutes (bromide (a tracer), toluene (a growth substrate), ethylene (a nontoxic surrogate substrate to probe for TCE transformation activity), dissolved oxygen (DO, an electron acceptor), and nitrate (nutrient)) into the aquifer. Push?Cpull blocking tests (PPBT) were also performed to examine whether the monooxygenase of toluene oxidizers is involved in the degradation of toluene and the transformation of ethylene. Through the NGDBT, NGDST, and PPBT, we confirmed that the addition of toluene and oxygen in these field tests stimulated indigenous toluene utilizers to cometabolize aerobically TCE, with the following results: (1) the observed simultaneous utilization of toluene and DO; (2) the transformation of ethylene to ethylene oxide and propylene to propylene oxide; and (3) the transformation of TCE. Furthermore, the results of restriction fragment length polymorphism suggested that the microbial community shifts and the microbes capable of transforming TCE are stimulated by injecting the growth substrate, toluene.     

Photocatalytic Oxidation of Reactive Red 22 in Aqueous Solution Using La2Ti2O7 Photocatalyst

This study was focused on the application of a highly doped layered perovskite, La₂Ti₂O₇, as the photocatalyst for the photocatalytic decomposition of an azo dye, Reactive Red 22 (RR22). The temporal behavior of the photocatalytic decomposition of RR22 in aqueous solution by the UV/La₂Ti₂O₇ with a batch photoreactor operated in a recirculation mode was studied under various operating conditions including solution pH, light intensity, and La₂Ti₂O₇ loading. The decomposition of RR22 in aqueous solution by La₂Ti₂O₇ photocatalytic process was found to be feasible. An empirical kinetic equation was developed for modeling the photocatalytic decomposition of RR22 in aqueous solution using UV/La₂Ti₂O₇ photocatalytic processes.     

Influence of Different Substrates in Wetland Soils on Denitrification

Different substrates were evaluated to investigate their effect on nitrate removal and denitrifying bacterial community in soils obtained from wetland. Serial batch kinetic tests were conducted on soils obtained from wetland mixed with glucose and sawdust using KNO₃ solution. Column tests were also conducted on soils obtained from wetland mixed with three different substrates (glucose, sawdust, and scoria coated with zero-valent iron) using KNO₃ solution. For the batch tests, the nitrate removal efficiency for soil mixed with glucose was comparable to that for soil mixed with sawdust, but the nitrate removal rate for soil mixed with glucose (23.3 NO ₃ ^? -N mg/L-d) was approximately eight times higher than that for soil mixed with sawdust (2.8 NO ₃ ^? -N mg/L-d). For column tests among soil samples, nitrate removal efficiency was highest in soil mixed with glucose, which is an easily biodegradable carbon source. Removal efficiency increased with increasing incubation time for both soil samples with glucose and sawdust. A phylogenetic analysis based on nitrate reductase gene demonstrated that the different carbon sources affected both the diversity and compositions of the denitrifying bacterial in soil samples.     

Spatial orientation in weightlessness and readaptation to earth's gravity

Unusual vestibular responses to head movements in weightlessness may produce spatial orientation illusions and symptoms of space motion sickness. An integrated set of experiments was performed during Spacelab 1, as well as before and after the flight, to evaluate responses mediated by the otolith organs and semicircular canals. A variety of measurements were used, including eye movements, postural control, perception of orientation, and susceptibility to space sickness.     

African wild ungulates compete with or facilitate cattle depending on season

Savannas worldwide are vital for both socioeconomic and biodiversity values. In these ecosystems, management decisions are based on the perception that wildlife and livestock compete for food, yet there are virtually no experimental data to support this assumption. We examined the effects of wild African ungulates on cattle performance, food intake, and diet quality. Wild ungulates depressed cattle food intake and performance during the dry season (competition) but enhanced cattle diet quality and performance during the wet season (facilitation). These results extend our understanding of the context-dependent-competition-facilitation balance, in general, and are critical for better understanding and managing wildlife-livestock coexistence in human-occupied savanna landscapes.