Uptake by dietary exposure and elimination of aflatoxins in muscle and liver of rainbow trout (Oncorhynchus mykiss)

Uptake and elimination of aflatoxins (AFs) by rainbow trout ( Oncorhynchus mykiss ) during a long-term (21 days) dietary exposure were studied to assess contamination by AFs in aquaculture fish fed AF-containing feed. The uptake factor (UF) of aflatoxin B(1) (AFB(1)) in muscle ranged from 0.40 × 10(-3) to 1.30 × 10(-3). AFB(1) concentrations in liver were 165-342 times higher than in muscle. AFs from feed were more highly accumulated in liver than in muscle. Aflatoxicol (AFL) and aflatoxin M(1) (AFM(1)) were detected in muscle and liver and also in the rearing water. AFL concentrations were higher than AFM(1) by 2 orders of magnitude in muscle, and AFL was a major metabolite of AFB(1). The elimination rate constants (α) of AFB(1) and AFL in muscle (1.83 and 2.02 day(-1), respectively) and liver (1.38 and 2.41 day(-1), respectively) were very large. The elimination half-life (t(1/2)) of AFB(1) was 0.38 days (9.12 h) in muscle and 0.50 days (12.00 h) in liver. The elimination half-life of AFL in muscle and liver was 0.34 day (8.16 h) and 0.29 day (6.96 h), respectively. These data show that AFs are eliminated rapidly and are not biomagnified in fish. Thus, AFB(1) concentration in muscle of fish fed AFB(1)-containing feed (ca. 500 μg/kg) decreased to below the detection limit (20 ng/kg) of the most sensitive analytical method at 1.54 days (36.96 h) after the change to uncontaminated feed.     

Effect of potassium rate on growth,quality, chemical composition,and winter hardiness of perennial ryegrass

Seven potassium (K) rates (0, 50, 150, 350, 450, 550, and 650 kg K/ha) were used to determine the optimum rate of K for turf‐type perennial ryegrass during the winter based on turf growth, quality, and winter hardiness. Turf density, color, growth, and winter hardiness were increased as the rate of K application increased up to 350 kg K/ha. However, there were no beneficial effects of K application beyond that rate. Potassium content of the whole plant was 1.72% of the dry matter at the 350 kg K/ha rate. Calcium and Mg contents of the plant were reduced as the result of K application, and a reduction in growth and K content was detected at the higher K application rates. It is suggested that the optimum K rate for perennial ryegrass is about 350 kg K/ha under our experimental conditions in the winter, and that the K content should be about 1.72% of dry weight of whole plant.     

Effect of fertility ratios on growth and turf quality of perennial ryegrass (Lolium prenne L.) in winter

Perennial ryegrass (Lolium prenne L.) has been introduced to Japan as a cool season turfgrass. In spite of relatively mild winter in most parts of the country, it still goes to winter dormancy. A well‐balanced fertility ratio and rate may prevent such dormancy without causing winter injury. Therefore, this experiment was designed to find a balanced rate and ratio among nitrogen (N), potassium (K), and phosphorus (P) fertilizers in order to prevent winter dormancy and promote growth during the winter in Japan. Yorktown 2 perennial ryegrass was used in this experiment. Nitrogen and K application rates were 50, 150, 250, 350, 450, 550, and 650 kg K/ha; whereas P rates were 50, 150, and 250 kg P/ha. These rates were applied in 30 different ratios. Growth, color, and density of the turf were estimated and used as the parameters for finding a suitable rate and ratio for these three major nutrients. Color, density, and growth were increased as the rate of N fertilizer increased, but there was no visual response to P or K rates. It is suggested that increases in color, density, and growth were function of N rates, whereas K and P rates maintained the cold hardiness of the turf when high N rates were applied under the conditions of this experiment. Fertility ratio and the rates of 450 kg N/ha, 250 kg K/ha, and 50 kg P/ha were sufficient to prevent dormancy, promote growth, and produce good quality turf throughout the winter. Thus, it is concluded that fertility ratios and rates are the main factors limiting growth and quality of perennial ryegrass in transitional zones similar to Japan.     

Ultrastructural studies of spermatia of grey mould (<Emphasis Type="Italic">Botryotinia fuckeliana</Emphasis>)

To investigate maturation of spermatia of Botryotinia fuckeliana, we examined micro-structural changes in spermatia in liquid culture. Three to four spherical lipid bodies were seen in the center of the cell at the start of culturing. After 3–7 days, maturing spermatia had developed large mitochondria along with extensively developed granular endoplasmic reticulum. In contrast, the lipid bodies decreased not only in number but also in size over time. The results obtained here suggest that the maturation of spermatia involves active cell metabolism using the energy supply from lipid bodies.     

Growth of Glucose-uptake-deficient Mutant of Xanthomonas oryzae pv. oryzae in Rice Leaves

We isolated Xanthomonas oryzae pv. oryzae mutants deficient in the phosphoenolpyruvate : carbohydrate phos-photransferase system, a major glucose transport system in bacteria, using the glucose analogue 3-deoxy-3-fluoro-d-glucose (3FG). Glucose uptake by the mutants was decreased to 15–35% of the parental strain, and growth greatly decreased in synthetic media containing glucose as a sole sugar source. Growth of the mutants in rice leaves was, however, similar to the wild type. These findings suggest that glucose is not necessarily a major carbohydrate source for X. o. pv. oryzae in rice leaves. Received 11 August 2000/ Accepted in revised form 15 December 2000     

Effect of water‐soluble carbohydrate content in orchardgrass pasture on grazing time and rumen fermentation in dairy cows

Two experiments were conducted to clarify the effect of water‐soluble carbohydrate (WSC) content in orchardgrass pasture on the diurnal distribution of grazing time. Six ruminally cannulated, non‐lactating dairy cows were grazed on either of two pastures with different orchardgrass cultivars containing low WSC (LWSC; cultivar: ‘Hokkai 28’) or high WSC (HWSC; cultivar: ‘Harunemidori’). The cows were grazed in morning and evening sessions in experiment 1, whereas the cows were grazed throughout the day in experiment 2. In experiment 1, grazing time of the cows on HWSC was longer than that of the cows on LWSC (P < 0.01). This difference was larger in the morning session than in the evening session (pasture × grazing session: P < 0.05). Effects on herbage intake were similar to those on grazing time. In experiment 2, daily total grazing time was longer for the cows on HWSC than for those on LWSC (P < 0.05). The cows on HWSC spent a longer time grazing than those on LWSC in the morning between 03.00 and 09.00 hours (P < 0.01). The results indicated that prolonged grazing time in the period between dawn and early morning could increase daily herbage intake in cows grazed on pastures of orchardgrass cultivars with high‐WSC content.