植物基因启动子的克隆方法及其应用

植物基因的表达调控已成为分子生物学研究热点,启动子是基因表达调控的重要顺式元件,启动子的克隆对于研究基因表达调控、构建基因工程载体、表达目的蛋白有着重要的意义。启动子克隆的方法很多,从常用的启动子陷阱技术筛选启动子到PCR方法的应用,此后相继问世的一些基于PCR的克隆启动子技术,如载体锚定PCR、反向PCR、接头PCR、交错热不对称PCR等,为克隆启动子提供了更可靠,更合理的方法。本文着重介绍了几种植物基因启动子的克隆方法,分析了它们的优缺点,并展望了今后的研究前景。     

Wavelet Edge Detection Method Based-on Multi-scale Fuzzy Logic

A novel edge detection algorithm is proposed by implementing fuzzy image enhancement at different scales.The implement of this algorithm can reduce the negative influence bring from the non-maximum suppression algorithm which select only one threshold.The success of the approach depends on the selection of a general fuzzy operator(GFO) for the enhancement of contrast of region after smoothing of image at different scales.Consequently,it is more obvious that coarse intensity changes are obtained at a large scale whereas the fine details of intensity changes are obtained at a small scale.A pretty performance is obtained by used this work.     

A Study on the FMS Planning and Scheduling System

After researching the function requirements of the planning and scheduling in manufacturing cells, the paper advanced a control structure with three phases, i. e. planning , scheduling and dispatching. The paper represented in detail the main purposes, requirements and scheduling actions in each phase. It suits to general machining FMSs.     

内混式自吸离心泵自吸性能影响因素的试验研究

针对比转速为74~112的5台内混式自吸离心泵,采用3种不同直径吸水管和6种不同隔舌间隙,对其规定高度自吸时间和极限真空度进行了较全面的试验研究,并对试验结果进行了综合分析。结果表明,对于内混式自吸离心泵,规定高度自吸时间总体上随吸水管内初始存气量的增加而成线性递增,而极限真空度与吸水管内的初始存气量无关;当隔舌间隙为1~6 mm时,隔舌间隙越小,自吸性能越好;泵的额定流量越大,自吸性能受隔舌间隙的影响越大。该研究对内混式自吸离心泵的水力设计、部分结构参数的确定以及自吸性能的进一步提高具有较重要的意义。