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将用于厌氧消化反应器的载体经好氧挂膜预处理,采用有机负荷递增法启动厌氧附着膜膨胀床(AAFEB)反应器,好氧预处理后的反应器比对照提前15天完成启动,并且具有较好的耐冲击负荷能力和耐水力负荷能力。这与污泥结构良好,持留能力强,污泥流失量小呈正相关。在反应器的稳态运行和再启动运行期间,反应器的主要运行参数与对照反应器无明显差异。 相似文献
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FEM技术用于车门设计的研究 总被引:3,自引:0,他引:3
叶德涛 《拖拉机与农用运输车》2006,33(4):18-19
介绍了解决有限元FEM(fin ite elem entm ethod)技术用于某小型运输车车门设计的关键问题的方法。对没有用加强板进行加强的车门进行了下沉工况静力分析和自由状态的模态分析,得到了车门的固有频率及其相应振型,对车门的静刚度和动刚度做出了评价,为进一步设计提供了参考。 相似文献
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‘桂红’是从野生中华猕猴桃后代中选育出来的猕猴桃新品种。平均单果重94.4 g。果实短圆柱形,果顶平或微凸;果皮黄绿色、薄,果毛残存、柔软,果斑黄褐色。果肉外缘金黄色,中轴白色,子房桃红色,呈放射状,肉质细,汁多,清甜,有香味,干物质含量17.39%,可溶性固形物含量16.70%,可溶性糖含量10.69%,可滴定酸含量1.56%,维生素C含量994.3mg/kg,含18种氨基酸,含包括硒在内的多种矿物质。在广西桂林地区,果实9月中下旬成熟。 相似文献
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AIM: In this study, CD147 antibody was used to carry out targeted modification of nanoparticles for protein kinase Cε (PKCε)-siRNA gene therapy to target lung cancer cells. The inhibitory effects of the nanoparticles on the proliferation and invasion of the lung cancer cells were observed. METHODS: The magnetic nanoparticles targeting CD147 protein were assembled as gene vector. The expression of CD147 in the lung cancer cells was observed under laser scanning confocal microscope. The cells were divided into CP group, CN group and LP group as the experimental groups. Targeted nanoparticles were used as CA group. Non-transfected cells were used as control group. The cell transfection was carried out with 250 ng plasmids/well in 6-well plate. The effect of nanocontrast agent on the cell endocytosis was observed under laser scanning confocal microscope. The mRNA expression of PKCε was detected by RT-qPCR. The protein expression of Ki67, MMP3, PKCε, Wnt1 and GAPDH was determined by Western blot. The cell proliferation ability was detected with colony formation assay. The cell invasion ability was detected by Transwell method. RESULTS: The expression of CD147 protein in the human lung cancer A549 cells was confirmed by immunofluorescence staining. The endocytosis of siRNA into the A549 cells in CP group was observed with the highest efficiency as compared with CN group and LP group. The relative mRNA expression of PKCε in the A549 cells of CP group, CN group, LP group and CA group were (9.76±0.18)%, (98.51±0.32)%, (99.17±0.16)% and (99.68±0.11)%, respectively. The difference between CP group and control group was statistically significant (P<0.05). No significant difference among CN group, LP group and control group was observed. The protein expression of PKCε, Ki-67, MMP3 and Wnt1 in CP group was significantly reduced, and the protein expression levels among CN group, LP group and control group had no significant difference. The colony number in CP group was significantly smaller than that in control group (P<0.05). The effective colony numbers in CN group, LP group and CA group had no significant difference as compared with control group. The number of the invading cells in CP group was significantly less than that in control group (P<0.05). The numbers of the invading cells in CN group, LP group and CA group had no significant difference as compared with control group. CONCLUSION: Nanogene vector targeting CD147 can carry PKCε-siRNA to conduct gene therapy efficiently on the lung cancer cells to achieve effective inhibitory effects on the proliferation and invasion of the lung cancer cells. 相似文献
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YE Xing-guo 《中国农业科学(英文版)》2015,(3):411-413
Genetic transformation is a powerful biotechnology for introducing novel genes into economically important plants from distantly-related plants or even unrelated species such as microbes and animals.This feat is impossible to be achieved by conventional breeding techniques.Development of transgenic plants has been a controversial subject since 1971 when the first genetically modified organism(GMO)was developed(James and Krattiger1996).Afterwards,transgenic study of plants has sparked 相似文献