Research Progress of Epigenetics Modification in the Process of Skeletal Muscle Cell Proliferation and Differentiation

Skeletal muscle is the most abundant tissue and the main component of muscle in animals.The process of skeletal muscle cell's proliferation and differentiation is the basis of muscle development and it's directly affects the meat production performance of domestic animals.It has been found that epigenetic modification plays an important role in regulating the proliferation and differentiation of skeletal muscle cells.In this study,the effects of epigenetics on skeletal muscle in terms of the effects of DNA methylation on the proliferation and differentiation of skeletal muscle cells,the selection and expression of factors regulated by histone acetylation,the regulation of non-coding RNA,and the effects of chromosome remodeling.Research progress in the process of muscle cell proliferation and differentiation,briefly describes the effects of different modification methods and factors on the two processes of skeletal muscle proliferation and differentiation.At the same time,the methods and means used by predecessor in the study of skeletal muscle proliferation and differentiation were reviewed,and then the role of apparent regulatory factors in skeletal muscle growth was analyzed.The purpose was to further explain the important role of epigenetic modification in the proliferation and differentiation of skeletal muscle,enhanced the understanding of the regulation of skeletal muscle proliferation and differentiation,provided a reference path for integration with animal production,and also provided skeletal muscle.Molecular regulation of such as growth and development provided more reference materials.     

规模化野猪养殖场仔猪腹泻病原菌的分离鉴定和致病性分析

从重庆某一规模化野猪养殖场腹泻仔猪的粪便棉拭子中分离到5株细菌,经形态学观察、染色特性、培养特性、生化特性分析鉴定为大肠杆菌,分别命名为CQ0401、CQ0402、CQ0403、CQ0404和CQ0405。并进行了溶血性、对Vero细胞的细胞毒性、小鼠致病性、抗药性等试验,试验结果表明:这5株大肠杆菌不产溶血素;腹腔接种小白鼠具有高致病性;抗菌谱基本一致:对菌必治(头孢三嗪)、复达欣(头孢他啶)、先锋必(头孢哌酮)敏感,尤其对菌必治敏感性最高,而对其他的药物敏感性较低或不敏感;CQ0401、CQ0403、CQ0404、CQ0405对Vero细胞均无细胞毒性,而CQ0402对Vero细胞具有较强的细胞毒性。     

种子处理对籽瓜细菌性果斑病防治效果的研究

籽瓜细菌性果斑病(Acidovorax avenae subsp.citrulli)是近年来在新疆出现的一种检疫性病害,种子带菌是该病传播的重要方式之一.通过对市售的带有细菌性果斑病的籽瓜种子进行不同温度及不同药剂的处理,经温室种植测定,计算出各种处理方法对籽瓜果斑病的防治效果.结果显示50℃湿热处理30min;60℃干热处理4h,以及2%的HCl处理20min对防治籽瓜细菌性果斑病种子带菌具有良好的效果,各生产单位可以根据实际情况选择合适的方法,以减轻细菌性果斑病的危害和蔓延.     

特异抗体致敏的SPA茵体浓缩新城疫病毒方法在临床诊断中的应用

为提高新城疫临床诊断的准确率,建立了新城疫病毒浓缩方法,将粪便及脑、肺等器官组织病料中的新城疫病毒经特异性抗体致敏的金黄色葡萄球菌(SPA菌)吸附浓缩后,用RT-PCR进行检测.结果表明,所建立的新城疫病毒浓缩方法应用于临床诊断后,可显著提高检测敏感性,从而提高临床诊断的准确率.     

Relationship between expression of MKK-4 and MMP-9 genes in primary hepatic carcinoma with or without invasion and metastasis

AIM：To investigate the expression of mitogen activated protein kinase kinase 4 (MKK-4) and MMP-9 mRNA in primary hepatic carcinoma (PHC), and to analyze its relationship with invasion and metastasis. METHODS：The expression of MKK-4 and MMP-9 mRNA in 34 cases of hepatic carcinoma tissues and adjacent tissues,and in 12 cases of normal liver tissues were detected with RT-PCR. RESULTS：(1) The expression level of MMP-9 mRNA was higher in metastatic cancer tissues than that in other tissuses (P<0.01). (2) There was significant statistical difference among the expression level of MKK-4 mRNA, but the level in metastatic cancer was low (P<0.01). (3) There was no statistical difference among the expression level of MKK-4 or MMP-9 mRNA among the adjacent tissues and normal tissues (P>0.05). (4) MMP-9 mRNA had a tendency to rise as PHC became invasive and metastatic.The expression level of MKK-4 had a tendency to decline in PHC became invasive and metastatic. (5) The expression level of MMP-9 or MKK-4 mRNA had no correlations with tumor volume,or cell differentiation (P>0.05). (6) There were correlations between expressions of MKK-4 and MMP-9 mRNA in PHC (Pearson Correlation, r=-0.925, P<0.01). CONCLUSION：There are high MMP-9 mRNA expression and low MKK-4 mRNA expression in PHC.The expression level of MKK-4 or MMP-9 mRNA is correlated with tumor metastasis.     

Expression of nerve growth inhibiting factor Nogo-A mRNA and protein in the brain ischemic infarction rats

AIM: To investigate the changes of expression of Nogo-A at different time points in brain ischemic infarct rats. METHODS: The model of 80 cases of middle cerebral artery occlusion (MCAO) in rats was established. The expression of Nogo-A mRNA and protein were determined by Western blotting and hybridization, and the relationship between functional scoring and Nogo-A was also evaluated. RESULTS: In the brain of MCAO rats, Nogo-A mRNA expression was decreased on day 3 and increased significantly on day 7. The highest level was observed at the 21th d, keeping the same level at the 28th d. Nogo-A protein expression showed the same results. These results were correlated with the brain function scoring. CONCLUSION: Expression of Nogo-A does not increase in the early stage, but increases significantly in the late stage of MCAO, suggesting that Nogo-A expression may play an important role in the nerve regeneration of brain ischemic injury.     

氮素形态对新高梨生长和果实品质的影响

在盆栽条件下,研究了氮素形态对新高梨生长和果实品质的影响.结果表明,培养液中铵态氮和硝态氮混合比例为50∶150时,新高梨的新梢生长、新梢数和叶面积增加显著;叶片叶绿素含量、平均单果重和可溶性固形物含量随培养液中硝态氮浓度的提高而增加,而果实酸度和果实硬度变化与可溶性固形物含量变化趋势相反.     

新疆莎车县棉田自然天敌数量动态

2002-2004年研究了新疆莎车县棉田节肢动物类自然天敌种群动态及棉花品种的影响。结果发现节肢动物类自然天敌67种,数量较多的共10类,即瓢虫类、蝽类、隐翅虫类、蓟马类、草蛉类、食蚜双翅目、胡蜂类、蚜茧蜂类、蜘蛛类和捕食螨类。其中瓢虫类、蝽类和蜘蛛类占天敌个体总数85%~93%。3年中这三类天敌各自所占比例,瓢虫为4%~65%,食虫蝽为19%~57%,蜘蛛为6%~34%。瓢虫、食虫蝽和蜘蛛的最高密度2002年分别为237.2、164.0、87.0头/百株,2003年分别为28.3、533.3、232.0头/百株,2004分别为1506.5、237.0、55.3头/百株。对转基因抗虫棉GK19(表达Cry1A杀虫蛋白)、SGK321(表达Cry1A CpTI杀虫蛋白)和普通棉花泗棉3号、石远321的研究表明,各种天敌在不同品种上的种群数量没有显著性差异,而喷施化学农药可以造成天敌数量明显减少。     

苏云金芽胞杆菌伴胞晶体蛋白对猪体内蛔虫的作用及其血液生理生化指标的分析

感染性猪蛔虫卵以每头份3000个卵的量感染断奶仔猪，于第3天开始肌肉注射不同剂量苏云金芽胞杆菌晶体蛋白（insecticidal crystal proteins，ICPs），每天1次，连续4d。同时测定猪血液生理生化指标，饲喂2个月后收集猪粪便计算虫卵数。结果显示，感染猪出现咳嗽、发热等临床症状，GOT、GPT、ALP、LDH等指标明显升高，经ICPs治疗后又恢复正常。粪便检查，ICPs高剂量组（16．08mg）的EPG（每克粪便虫卵数）为0，而ICPs低剂量组（9．45mg）的EPG为394，未经ICPs作用对照组EPG为2113，差异极显著（P〈0．01），证明ICPs对猪体内猪蛔虫具有较好的杀灭作用。