中国椰子耐寒生理研究进展与展望

低温是限制椰子种植业发展的主要因素,严重制约着中国椰子产业的发展。综述了寒害对中国椰子产业的影响,以及椰子抗寒生理的研究现状,并根据已经开展的棕榈科植物抗寒生理研究的情况,对椰子抗寒生理的研究进行了展望。     

农杆菌浸润瞬时表达分析番木瓜miR162a的研究

农杆菌浸润瞬时表达方法不仅在蛋白互作、鉴定基因沉默抑制子及基因功能研究方面得到广泛应用,目前还应用在小RNA的研究中.在对植物microRNA的研究中,农杆菌浸润方法通过将将转基因导入农杆菌浸润本生烟瞬时表达,可以快速、稳定地对植物microRNA进行检测和鉴定,样品在几天内就可分析完成,比获得稳定遗传的转基因有优势.笔者借鉴国外拟南芥microRNA瞬时表达分析的方法,克隆了番木瓜的miR62a前体序列连接到双元表达载体pSuper1300上构建成35S::cpa-miR162a质粒,导人农杆菌GV3101后浸润本生烟,浸润后48 h提取本生烟叶片总RNA后通过miRNA northern blot检测到miR162a,而本生烟和番木瓜自身则无法检测到低丰度的miR162a.本文介绍的农杆菌浸润瞬时表达方法能够在体外加工产生成熟的miRNA,该技术有助于对microRNA的结构-功能的相关性及靶标验证进一步研究.     

中国传统木结构在继承和创新发展中的问题分析

回顾总结我国传统木结构建筑的发展历史,并对我国传统木结构建筑及其文化的传承、推广进行深入分析;针对传统木结构发展瓶颈,提出我国传承和发展传统木结构的主要课题,指出现代科技在传统木结构发展中的重要作用,并从科研投入、人才培养、产学研合作及产业化等方面,对我国传统木结构的发展提出建议。     

研究型学院本科教学运行管理体系的构建与实践

研究型学院以培养创新人才和发展研究型教学为核心，构建适应研究型学院本科教学运行管理体系是实现这一核心任务的根本保证。通过探索和实践，初步构建了适合研究型学院的，能有效确保教学正常运行的管理体系，实现教学管理科学化、规范化、制度化，不断提高人才培养质量和管理水平。     

杂交中籼稻金优107高产制种技术

介绍杂交中籼稻金优107的高产制种技术,包括选择制种基地、合理安排播插期、培育高产群体、搞好花期预测、严格去杂等方面内容,以为该品种的制种提供参考。     

隆林油茶生产现状及发展对策

通过对隆林县油茶种植情况调查研究,分析目前隆林油茶生产中普遍存在的品种混乱、密度过大、管理粗放、落花落果严重、病虫害严重等主要问题,并通过对比试验提出今后油茶生产发展中的具体对策。     

现代产品中人机界面设计的研究——基于以人为本的视角

人机界面是用户使用者和计算机交流所需要的互动媒介,是对软件的人机交互、操作逻辑、界面美观的整体设计,是一个不断为用户设计满意视觉效果的过程。它有助于实现信息的内部形式与用户可接受形式之间的转换,是一个复杂的多学科相互交融参与的工程。从人机界面设计的意义、过程和优化等方面研究现代产品中的人机界面,并对现代人机界面设计方法进行了总结。     

花铃期淹水对棉花生长、生理和产量的影响

 以陆地棉（Gossipium hirsutum L.）品种K638为材料，在自动遮雨棚内研究了花铃期淹水8 d和10 d对棉花生长、生理和产量等的影响。结果表明，与未淹水的对照相比，淹水8 d、10 d的棉花产量降低了23.5%, 26.5%，单株干物质质量和叶面积分别降低了15.5%, 19.4%和22.6%, 36.1%，单株结铃数和铃重分别降低了26.3%, 28.8%和9.5%,9.9%，脱落率和烂铃率分别提高了11.1%, 12.7%和66.7%,111.1%，叶片光合速率降低了11.6%, 17.2%，叶绿素含量和叶绿素a/b的值分别减少了13.5%, 25.0%和3.3%, 8.5%；可溶性糖含量和MDA含量分别升高了3.4%, 8.5%和9.7%, 12.9%；可溶性蛋白和Bt蛋白含量分别减少了6.4%, 10.3%和8.7%, 14.1%。可见，淹水显著影响了棉株的生长发育和生理代谢，导致减产；淹水时间越长，不利影响越大，减产幅度也越大。     

新世纪“图书馆有偿服务”研究综述

有偿服务一直是图书馆界的热门话题。综观新世纪以来关于"图书馆有偿服务"的研究脉络,其主要内容大致涵盖以下几大部分:"有偿服务的研究脉络与发展趋势"、"收费价格"、"收费管理"等。     

Comparison of 2 methods for establishing rat model of chronic osmotic diarrhea

AIM: To compare 2 different rat models of chronic osmotic diarrhea with the lactose enrichment and magnesium citrate methods. METHODS: The SD rats (n=31) were randomly divided into lactose enrichment diarrhea group (Lac group, 12 rats), magnesium citrate diarrhea group (Mg group, 12 rats) and normal control group (Con group, 7 rats). The rats in Lac group were fed with a lactose enrichment diet and freely drank water. The rats in Mg group were fed with a standard diet and drank magnesium citrate solution. The Con group was fed with a standard diet and normal water. The general conditions and the characteristics of stool were observed every day. The grip strength and motor activity of the rats were measured after diarrhea for 14 d. The trans-epithelial resistance of the colonic mucosa was detected by Ussing chamber. The pathological changes of villus height and villus surface area in the intestine were observed with hemato-xylin-eosin staining. RESULTS: The rats in both diarrhea groups displayed the clinical manifestations of slow increase in body weight, spare and dull hair, lacking in strength and physical inactivity. The grip strength and motor activity of the rats in both diarrhea groups decreased significantly compared with Con group. The diarrhea rate and diarrhea index of the rats in the 2 diarrhea groups were both significantly higher than those in Con group (P<0.05). Moreover, the diarrhea index in Lac group was significantly increased compared with Mg group. The thickness of intestinal mucosa in Lac group was thinner than that in Mg group at the same time. However, the damage of intestinal villi in Mg group was more serious than that in Lac group.CONCLUSION: Both methods of lactose enrichment-induced diarrhea and magnesium citrate-induced diarrhea meet the requirement of chronic osmotic diarrhea, but there are different advantages between the diarrhea models. Therefore, we should select the correct diarrhea model to meet different aims of the experiments.