氰戊菊酯对草鱼肝微粒体CYP3A活性的影响

通过设计1.2、6.0、12.0μg/L 3个氰戊菊酯质量浓度组,1个空白对照组,1个恩诺沙星阳性对照组,进行氰戊菊酯对草鱼肝微粒体CYP3A活性影响及草鱼CYP3A活性的组织分布研究。结果表明:CYP3A存在于肝脏、鳃、肾脏、脾、肠、脑组织中,其中肝脏中活性最高;氰戊菊酯能显著抑制CYP3A的活性,随暴露时间的延长抑制作用增强,但抑制趋势减弱,3 d内趋势最强,各试验浓度组之间的差异不显著。建议临床用药,尤其是联合用药、多次用药时要充分考虑氰戊菊酯对代谢酶活性的影响。     

东祁连山高寒植被土壤解磷菌筛选及其解磷能力的初步研究

采用PKO固体培养基从东祁连山高寒草地中分离出具有解磷能力的菌株33株,对其进行继代培养,得到13株功能稳定的解磷菌,采用2种方法测定其解磷能力。13株解磷菌在PKO平板上8 d后,解磷圈D/d值为1.21~4.01;在液体摇瓶中,其解磷率在0.13~11.842 mg/g。其中,菌株2-2-1具有较高的解磷率,解磷效果在1%水平下显著高于其他菌株,具有较大的应用潜力。     

碳化微米木纤维模压DPF设备的研究与实验

介绍了以碳化微米木纤维模压DPF的技术及工艺,研究了微米木纤维模压制品的制备方法,设计了专用模压设备,阐述了实验方法,获取了关键工艺参数,为产品产业化推广奠定了基础.     

医院制剂伤科灵膏含量测定

[目的]建立伤科灵膏的高效液相色谱法含量测定方法。[方法]采用BP-NH2色谱柱测定伤科灵膏中苦参碱和氧化苦参碱的含量;流动相为乙腈:异丙醇:3%磷酸溶液(72.0∶4.5∶23.5),检测波长210 nm,流速0.8 m L/min。[结果]该含量测定法分离度较好;苦参碱在0.248~1.160μg范围内呈良好的线性关系,平均加样回收率为97.64%(RSD=1.25%);氧化苦参碱在0.904~4.068μg范围内呈良好的线性关系,平均加样回收率为97.21%(RSD=1.84%)。[结论]建立的方法简便、可行、灵敏、准确,可以用于伤科灵膏的质量控制。     

纤维素分解功能菌及其在草业系统界面中的利用潜势

草业系统界面论完善了草业科学的理论,为分析草业生产中系统耦合和系统相悖提供了新的方法论。本研究就国内外关于纤维素分解功能菌方面的相关报道,从纤维素分解菌的物种资源、微生物分泌的纤维素酶资源、纤维素酶基因资源3个方面总结纤维素分解功能菌的资源优势。以系统论和草业系统界面论为理论依据,从草地农业系统中的3个界面,即草丛-地境界面(A)、草地-动物界面(B) 和草畜-经营管理界面(C),分析纤维素分解功能菌资源在草业系统中的利用潜势,认为纤维素分解功能菌在土壤有机质分解、资源循环利用、环境保护、缓解草畜矛盾等方面具有一定的应用前景。并提出3个发展方向：1）继续以生态学研究为基础,研究纤维素分解菌多样性和生态功能的相互关系;2）选育对牧草及农作物秸秆具有高效降解作用的优良菌株,通过非传统的农业方法来解决家畜养殖中的饲料来源;3）促进家畜-作物系统深度耦合,维持整个草地农业生态系统有机协调地持续发展。     

芽孢杆菌B_1对“银帝”甜瓜主要采后病害的抑制

研究了芽孢杆菌B1原液、滤液、灭菌液和悬浮液对“银帝”甜瓜主要采后病害的抑制效果。结果表明,B1原液能显著的抑制损伤接种甜瓜果实的白霉病、粉霉病、软腐病和黑斑病,其抑制率分别为75.9 %、78.7 %、100 % 和99 %。B1滤液仅对白霉病病斑面积的扩展有所抑制,但对该病发病率及粉霉病的抑制效果不显著。B1杀菌液可有效降低黑斑病的发病率与病斑面积,减轻白霉病和粉霉病病斑面积的扩展,但对这两种病害的发病率影响不大。B1悬浮液仅能抑制粉霉病及白霉病病斑面积。     

薏苡仁红枣保健饮料的研制

[目的]研究薏苡仁红枣保健饮料的液化和浸提制汁工艺。[方法]薏苡仁乳与红枣汁用量比、糖度、酸度做三因素三水平正交试验1,由10人评分小组按感官评分,选出较优水平组合。选取XGM和CMC作为稳定剂,蔗糖酯和单甘酯作为乳化剂,做四因素三水平正交试验2,选出较优水平组合。[结果]根据正交试验1和2的结果,确定产品最佳基料配方为:薏苡仁乳(料水比1∶6)和红枣汁(料水比1∶7)比为1∶2。辅料:蔗糖6%,柠檬酸0.25%,XGM 0.10%,CMC 0.12%,蔗糖酯0.08%。[结论]按该配方及工艺流程操作,完全去掉了薏米特有的令人不快的气味,制成口感优良、风味独特的美容保健饮料。     

Inoculation with chlamydospores of Trichoderma asperellum SM-12F1 accelerated arsenic volatilization and influenced arsenic availability in soils

Fungi capable of arsenic(As) accumulation and volatilization are hoped to tackle As-contaminated environment in the future. However, little data is available regarding their performances in field soils. In this study, the chlamydospores of Trichoderma asperellum SM-12F1 capable of As resistance, accumulation, and volatilization were inoculated into As-contaminated Chenzhou(CZ) and Shimen(SM) soils, and subsequently As volatilization and availability were assessed. The results indicated that T. asperellum SM-12F1 could reproduce well in As-contaminated soils. After cultivated for 42 days, the colony forming units(cfu) of T. asperellum SM-12F1 in CZ and SM soils reached 1010–1011 cfu g–1 fresh soil when inoculated at a rate of 5.0%. Inoculation with chlamydospores of T. asperellum SM-12F1 could significantly accelerate As volatilization from soils. The contents of volatilized As from CZ and SM soils after being inoculated with chlamydospores at a rate of 5.0% for 42 days were 2.0 and 0.6 μg kg–1, respectively, which were about 27.5 and 2.5 times higher than their corresponding controls of no inoculation(CZ, 0.1 μg kg–1; SM, 0.3 μg kg–1). Furthermore, the available As content in SM soils was decreased by 23.7%, and that in CZ soils increased by 3.3% compared with their corresponding controls. Further studies showed that soil p H values significantly decreased as a function of cultivation time or the inoculation level of chlamydospores. The p H values in CZ and SM soils after being inoculated with 5.0% of chlamydospores for 42 days were 6.04 and 6.02, respectively, which were lowered by 0.34 and 1.21 compared with their corresponding controls(CZ, 6.38; SM, 7.23). The changes in soil p H and As-binding fractions after inoculation might be responsible for the changes in As availability. These observations could shed light on the future remediation of As-contaminated soils using fungi.     

Inoculation with chlamydospores of Trichoderma asperellum SM-12F1 accelerated arsenic volatilization and inlfuenced arsenic availability in soils

Fungi capable of arsenic (As) accumulation and volatilization are hoped to tackle As-contaminated environment in the future. However, little data is available regarding their performances in ifeld soil...     

微生物肥对高油玉米叶部生理指标及产量性状的影响

自然环境下施用微生物肥和复合肥,探索微生物肥料对高油玉米叶部生理特性和产量性状的影响。结果表明：施用微生物肥后,高油玉米叶绿素含量比施复合肥叶绿素含量要高,对玉米的光合作用有一定的改善;能改善玉米叶片的抗脱水能力,增加叶片束缚水含量,降低自由水含量与束缚水含量的比值;增加玉米叶片细胞内游离脯氨酸和玉米叶片蛋白质含量,提高叶片过氧化物酶活性,对高油玉米产量性状有明显改善作用,有效协调了产量的构成因素。