甘蓝NLR家族全基因组鉴定、进化分析及在不同病害胁迫下的表达分析

从全基因组水平鉴定了87个甘蓝NLR基因家族成员,并对其进行了进化分析和表达分析。基因结构和motif组成分析表明,87个基因可分为5个亚类:NB-LRR(34个)、CC-NB-LRR(6个)、TIR-NB-LRR(45个)、RPW8-NB-LRR(1个)和CC-TIR-NB-LRR(1个);染色体定位分析表明,该家族基因在9条染色体上呈不均匀地单一或成簇分布;进化分析表明,甘蓝35个NLR基因与白菜NLR基因存在同源关系,其余52个基因为甘蓝特有;利用转录组数据分析了参与甘蓝枯萎病、黑腐病、根肿病、霜霉病、白粉病等抗性应答相关的差异表达基因,发现参与不同病害胁迫抗性应答反应的NLR基因差异较大,这可能与不同NLR基因对不同病原特异性识别有关。     

高职院校模具设计与制造专业的教学改革与应用

文章对传统模具教学中的不足进行分析介绍,同时结合学生的学习现状,为高校模具设计与制造专业的教学找到了切实可行的改革措施,最终实现了模具设计在社会生活中更好的应用。     

外源接种黄曲霉污染普洱茶安全性研究

以云南普洱茶为实验材料,外源接种黄曲霉(Aspergillus flavus)菌株于普洱茶原料及成品中,设未接种菌株普洱茶为对照,分别置于室温,湿度80%、温度30℃,湿度90%、温度30℃的恒温恒湿箱条件下存放,在第7天、14天、21天、28天分别取样以LC-MS/MS检测法进行黄曲霉毒素检测。检测结果表明,所有受试茶样中均未检测到黄曲霉毒素B1、B2、G1、G2,检出率为0。说明无论是室温还是在高温高湿条件下,被黄曲霉污染的普洱茶,经存放后,不会产黄曲霉毒素,就这一点而言普洱茶具有较高的饮用安全性。     

Effect of ClC-3 siRNA on cell cycle of HeLa cells

AIM: To investigate the roles of ClC-3 chloride channels in the regulation of cell cycle and the relationship between ClC-3 chloride channels and the cell cycle regulators, such as cyclin D1, cyclin-dependent kinase (CDK)4, CDK6, P21 and P27 in the HeLa cells.METHODS: ClC-3 genes were silenced by the siRNA technique in the HeLa cells. The transfection efficiency of ClC-3 siRNA was detected by real-time PCR. The cell cycle distribution was analyzed by the flow cytometry. The protein expression of ClC-3, P21, P27, CDK4, CDK6 and cyclin D1 was determined by Western blot.RESULTS: ClC-3 was knocked down by ClC-3 siRNA in the HeLa cells. Transfection of the cells with ClC-3 siRNA arrested the cells at G₀/G₁ phases, decreased the expression of cyclin D1, CDK4 and CDK6, and increased the expression of P21 and P27.CONCLUSION: ClC-3 plays an important role in the cell cycle of HeLa cells through the G₁-S transition point. ClC-3 may regulate the cell cycle progression by up-regulation of cyclin D1, CDK4 and CDK6 expression and/or by down-regulation of P21 and P27 expression.     

天坪石竹林的改造技术及石竹笋干的精制工艺

天坪石竹笋属天然绿色食品,风味独特,营养丰富,深受江浙人们的喜爱.天然石竹林多为野生状态,经济效益极低.对野生石竹林实施科学改造后,竹笋产量显著增加,笋干品质明显提高,经济效益大大提升.文章总结了野生石竹林的改造技术及石竹笋干的精制工艺,为石竹产品开发提供借鉴.     

红心材马尾松胚培养研究

采用正交试验设计，研究不同基本培养基和激素配比对红心材马尾松胚培养的影响。结果表明，沙子和无菌水培养基上的种子萌发率最高，达到71.6％，苗长得最好。WPM＋6 BA 2 mg/L＋NAA 0.3 mg/L＋KT 0.2 mg/L培养基为最佳增殖培养基，增殖系数最高，达到7.3，芽高度较高、粗壮。以1/2WPM＋ABT1＃0.8 mg/L＋NAA 0.1 mg/L培养基不定芽生根率最高，达到69.7％。     

都市屋顶绿化草坪草保水剂施用技术及经济效益分析

选取了北方地区常用草坪草高羊茅为研究对象,探究了保水剂在屋顶绿化草坪草栽培应用中的最优施用制度及经济效益。结果表明：在50%～70%FC水分控制下高羊茅生长所需的耗水量比70%～90%FC水分控制下的耗水量要小,且任意水分控制条件下,施用保水剂处理组的总耗水量均比对照组的总耗水量要小;当保水剂施用浓度为0.5%,施入层...     

不同栽培条件对中稻产量及生育进程的影响

对杂交中稻丰两优香1号进行不同播期、密度、施氮量和水分4因素3水平的正交试验,筛选其最佳栽培因子组合。结果表明,播期和施氮量是影响中稻生育进程和产量的主要因素;播期对中稻生育期影响显著,对产量影响极显著,施氮量对中稻产量影响极显著;中稻高产栽培的最佳组合的播期为5月1日、大田栽插秧苗密度为60万苗/hm2、施氮量为121.6kg/hm2、水分为10cm水层。     

枫香树脂浅色化工艺试验

应用水蒸气蒸馏工艺,通过添加抗氧化剂,研究制备浅色化枫香树脂的方法。试验表明:对枫香脂的前处理可有效的提高枫香树脂的颜色等级,同时添加抗氧化剂也可有效防止枫香树脂在蒸馏过程中的颜色加深。适宜的工艺条件为:枫脂精油做溶解油,溶解油加入量与枫脂质量比为2∶1,蒸馏水洗4次,单次洗涤水的用量与枫脂质量比为4∶1,常压水蒸气蒸馏温度为130~140℃,蒸馏时间为30 min,减压蒸馏时间为10 min,所得枫香树脂的颜色等级由简单直接蒸馏的12(加纳级)上升为6(加纳级),软化点为131℃,酸值为53 mg/g,皂化值为104 mg KOH/g。     

QTL Identification of the Insensitive Response to Photoperiod and Temperature in Soybean by Association Mapping

The insensitive response to photoperiod and temperature is an important quantitative trait for soybean in wide adaptation breeding. The natural variation in response to photoperiod and temperature was detected using 275 accessions of soybean [Glycine max (L.) Merrill] from China. Genome-wide association mapping, based on population structure analysis, was carried out using 118 SSR markers by the TASSEL GLM (general linear model) program. Nine SSR markers (P<0.01) were associated with the value of the response to photoperiod and temperature (VRPT) caused by days to flowering (DF), among which, Satt308 (LG M), Satt150 (LG M) and Satt440 (LG I), were identified in both 2006 and 2007. Twelve SSR markers (P<0.01) were associated with VRPT caused by days to maturity (DM), among which three markers, Satt387 (LG N), Satt307 (LG C2) and AW310961 (LG J), were detected in both 2006 and 2007. In addition, a total of 20 elite alleles were screened out over 2006 and 2007 for being associated with an insensitive response to photoperiod and temperature (IRPT) caused by DF and a total of seven different elite alleles were screened out for being associated with IRPT caused by DM. Among these elite alleles, five alleles, Satt150-244, Satt308-164, Satt308-206, Satt440-176, and Satt440-206, were associated with IRPT caused by DF and were identified in both years, but only one allele, Satt307-170, was identified as being associated with an IRPT caused by DM. Based on these elite alleles, a set of typical accessions were screened out. The result about the genetic basis of IRPT is meaningful for soybean wide adaption breeding.