Establishment of a Duplex Real-time RT-PCR Assay for the Differential Detection of Nipha Virus and Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

To establish a rapid,sensitive and specific assay for the differential detection of Nipah virus (NiV) and highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV),a duplex Real-time RT-PCR was developed with specific primers and probes targeting to the special sequences of NiV M gene and HP-PRRSV nsp2 gene by optimization of reaction conditions.The performance of the assay was linear ranging from 4.6×10¹ to 4.6×10⁷ copies/μL for RNA standard control of NiV M (NiV-M-RNA) and from 4.1×10¹ to 4.1×10⁸ copies/μL for RNA standard control of HP-PRRSV nsp2 (HP-PRRSV-nsp2-RNA),and detection limits of the assay was 46 copies for the NiV-M-RNA and 4.1 copies for the HP-PRRSV-nsp2-RNA,respectively.The coefficients of variation (CVs) of both inter-assay and intra-assay repeatability were less than 2.0%,showing good repeatability.The assay was able to specifically detect NiV and HP-PRRSV simultaneously without cross-reaction with classical swine fever virus (CSFV),porcine epidemic diarrhea virus (PEDV),swine influenza virus (SIV),porcine parvovirus (PPV),pseudorabies virus (PRV) and porcine circovirus type 2 (PCV2).Of the 236 samples from pigs for both NiV and HP-PRRSV detection by the established assay,all the samples were negative for NiV,8 samples were HP-PRRSV positive.In conclusion,this assay offers a useful approach for the differential detection of NiV and HP-PRRSV in clinical specimens from the pigs.     

苹果果实表皮总蛋白提取及双向电泳方法的优化

以苹果果实表皮为试材,采用3种方法提取果实表皮总蛋白,并对双向电泳(2-DE)操作体系进行优化。结果表明:改良酚提法适于苹果果实表皮总蛋白的提取,优化后的2-DE分离体系为预制IPG胶条18cm pH 4~7,上样量600μg,改进后的等电聚焦程序,浓度16%的分离胶,适于苹果果实表皮总蛋白的2-DE分离。     

天门市蔬菜产业发展现状及对策

分析了天门蔬菜产业发展现状,指出其发展过程中存在的问题,提出了加大基地配套设施建设力度、优化蔬菜品种结构、促进集约规模化生产、提高产品市场竞争力、完善质量安全保障体系、强化宣传监管、构建蔬菜市场流通体系的解决对策,以期为天门蔬菜产业的健康持续发展提供参考。     

农牧交错带灌溉和旱作模式下马铃薯耗水差异

为揭示北方农牧交错带地区马铃薯“水改旱”种植对其产量和水分利用的影响,选取中国北方农牧交错带地区27个站点,基于站点的气象数据、土壤数据和管理数据驱动充分验证的APSIM-Potato模型,模拟分析农牧交错带地区灌溉和雨养马铃薯的耗水差异。结果表明：北方农牧交错带地区马铃薯种植连续灌溉10、20 a和30 a的产量分别为15 900～35 600、16 400～34 800 kg·hm^-2和16 600～34 800 kg·hm^-2,改为旱作后对应的产量分别为12 800～30 600、13 900～29 100 kg·hm^-2和12 700～25 500 kg·hm^-2;灌溉马铃薯产量均表现为西部较高,旱作马铃薯产量则为东部较高。连续灌溉10、20 a和30 a的播前1 m土层土壤含水量分别为163～388、161～394 mm和154～398 mm,改为旱作后分别下降31.8%、35.3%和36.9%。连续灌溉10、20 a和30 a后的地下水消耗量分别为5 360～21 330、8 910～4...     

贮藏时间与蒙药黄芩对紫花苜蓿草颗粒维生素和化学成分的影响

为有效保全苜蓿草颗粒贮藏过程中维生素及营养品质,本研究以紫花苜蓿草颗粒为研究材料,研究贮藏时间(0,60,120,180,240 d,冷冻240 d)与蒙药黄芩(0%,0.5%,1.0%,1.5%)对其维生素含量和营养品质的影响。结果表明：在贮藏过程中,与其他添加量相比,添加1.5%黄芩有利于保存苜蓿草颗粒维生素B5、维生素C和α-维生素E含量。贮藏60 d及以上显著降低了苜蓿草颗粒维生素B1、维生素B3、维生素C和维生素E含量;贮藏120 d时苜蓿草颗粒DM含量趋于稳定;苜蓿草颗粒贮藏180 d后其CP含量显著降低;贮藏0～180 d期间,随着贮藏时间的延长,苜蓿草颗粒NDF和ADF含量显著升高。与常温贮藏240 d相比,冷冻贮藏相同时间有利于保存苜蓿草颗粒中维生素B1、维生素B5、α-维生素E和OM含量,显著降低DM、NDF和ADF含量。因此,1.5%黄芩添加剂有利于保存苜蓿草颗粒维生素含量,且常温贮藏时间不宜超过60天。     

地下热水的来源与补给机制分析——以重庆北温泉为例

地下热水的来源研究对地下热水资源量评价和可持续开发利用有重要意义。选取重庆市北温泉作为研究对象,采用水化学分析、D、O同位素以及实时在线监测对其热水来源进行了研究。研究认为：重庆市北温泉泉水为中-低温浅层中性地热水,水化学类型为SO4-Ca型,地下热水的δ 18O值为-8.48‰～-7.09‰,δD值为-55.46‰～-53.26‰,其补给来源为雨水,补给高程为海拔641～1 206 m的岩溶出露区。安装马歇尔槽和CDTP300高分辨率实时在线监测发现：1)地下热水水文地球化学特征在长时间内稳定,体现了稳定的水-岩作用过程;2)北温泉的流量与研究区降雨量呈现出以半年为单位的滞后,雨季偏低,旱季偏高;当场降雨后20 d左右,温泉水的水温和电导率微降,水量增加。地下热水的来源为大气降水,其补给除了长时间长距离的含水层稳定补给外,热水上升过程中受地表水和浅层地下水的补给。     

Optimality Conditions for Nonsmooth Extremum Problems in Topological Vector Spaces

Some new concepts of coneD_s convexity,coneD-s quasiconvexity,cone D-s pseudoconvexity,s right derivative of vector value mapping are proposed;and their related properties are discussed;the optimality necessary conditions and sufficient conditions for constrained extremum problem(VP)involving in cone D-s convex mapping are extablished;the relation between local optimal solution and global optimal solution;as well as the relation between weakly efficient solution and efficient solution for (VP) are derived;the corresponding results generalize the related results of classic convex programming.     

The research in processing fMRI data using temporal-spatial model based on CCA

In the analysis of cognition of human brain,the fMRI is often used.Corelation is a common method in fMRI data detecting,but its ability should be improved.Based on the Canonical Correlation Analysis(CCA),the temporal correlation and spatial correlation are used together to detect the activated area in the fMRI data.By the application of the method in simulation data and fMRI data,it shows that this method is effective in the detecting.     

Determination to Weighting Coefficient of Combination Forecast Based on Rough Set Theory

The problem of determination to weighting coefficient is a key and difficulty for combination forecast. A method of determining weighting coefficient based on rough set theory is showed in this paper. Determining weighting coefficient is translated into estimating significance of attributes among rough set. A relation data model about combination forecast is established. Knowledge systems are built through making attribute value into eigenvalue. Under data moving, the weighting coefficients of a combination forecast model are computed by analyzing the dependence and significance of forecasting method for the predicted object. The proposed approach overcomes the subjectivity of traditional determination to weighting coefficient, avoids computing linear or nonlinear extremum problem and makes combination forecast more objective. The validity of the proposed approach is verified with a case.