3个草莓品种在辽阳地区温室栽培比较试验

为优化辽阳地区温室栽培草莓品种,笔者于2018年8月在辽阳县亚新农业合作社进行'香野'红颜'甜查理'温室栽培试验.结果表明,新品种'香野'果实甘甜爽口、香味浓郁、口感好、可溶性固形物含量高、抗病性强,是一个早熟、高产、优质的草莓新品种,可作为'甜查理'的替代品种在辽宁辽阳地区种植.     

中国柞树主要害虫名录(Ⅲ)

记述了鳞翅目(Lepidoptera)灯蛾科(Arctiidae)和夜蛾科(Noctuidae)的柞树主要害虫75种,分别介绍了害虫的中文名称、学名、寄主种类及在我国的主要分布区域,为有效控制灯蛾类、夜蛾类柞树主要害虫的发生与危害提供相关的基础信息。     

低碳经济视野下森林旅游发展探讨

发展低碳经济已得到政府和社会各界的广泛关注,低碳经济已经成为经济发展的必然趋势。旅游业作为第三产业的重要组成部分,是低碳经济的有机组成部分。从需求意愿、驱动机制、开发模式和评价指标体系几个方面对森林旅游低碳化发展的研究内容进行探讨,以期对森林旅游低碳发展相关研究提供借鉴。     

23个欧美杨无性系苗期叶锈病抗性测定

以引进23个欧美杨无性系为研究对象,通过对苗木叶锈病感病指数的调查,借助SPSS统计软件对调查数据进行统计分析,结果表明:对叶锈病的抗性表现超过对照品种I108的有19个,其中02-36-1、02-36-4、N177、N197和N195这5个无性系对叶锈病完全免疫;无性系02-34-334对叶锈病表现为高度抗病;02-01-119、324、03-04-111、03-04-97和02-34-278表现为抗病;03-04-141、03-04-170、02-34-347和03-05-184表现为感病;02-01-219、03-04-167、03-04-288和02-34-228表现为高感;对叶锈病的抗性表现不及对照品种I108的有3个,即03-05-206、03-04-171和03-05-156,均表现为高感。     

对兽用生物制品试行规程、质量标准、说明书和内包装标签进行规范性修改的建议

针对目前因申报注册材料书写不规范而造成在申报过程中的反复修改、补充，以及因此给评审工作带来较被动局面的情况，结合工作实际，从内容和格式上分别对试行规程、质量标准、说明书和内包装标签提出了规范性修改的建议。     

DNA标记技术在梨属植物研究中的应用

综述了几种常见的DNA标记如RFLP、RAPD、SSR、AFLP和SCAR在梨属植物研究中的应用以及所取得的进展。DNA标记技术主要在梨属植物的品种鉴别、亲子关系鉴定、分类和系统关系研究、遗传图谱构建与标记辅助育种等研究领域中得到了广泛的应用。还对各种标记的特点和在梨属植物研究中的应用范围做了总结,期望为今后研究中合理选择适宜的标记技术提供参考。     

青藏高原植物香芸火绒草挥发油的超临界CO2萃取及GC-MS分析

通过对高原植物香芸火绒草超临界CO2萃取的研究，获得了温度45℃、压力18MPa为其相对较佳提取条件，提取产物为黄绿色浸膏，得率为0．87％。将该浸膏精制成净油，其得率为60．2％。进一步通过气相色谱-质谱联用技术（GC—MS）对净油分析，鉴定出其中的15种化学成分，并通过峰面积归一法确定了它们的相对含量。挥发油成分主要为：邻苯二甲酸异辛酯、高良姜素黄烷酮、α-甜没药萜醇、橙花叔醇、胡萝卜醇、棕榈酸等。通过对香芸火绒草挥发油成分的研究，为高原特有植物资源的进一步开发利用提供了试验依据。     

Effects of 4-hydroxytamoxifen on the growth and proliferation of prolactinomas CH3 cells

AIM: To investigate the effect of estrogen antagonists on the in vitro growth of human prolactinomas. METHODS: RT-PCR was applied to the detection of estrogen receptor (ER) mRNA expressed in a human prolactinomas CH3 cell strain. Estradiol and 4-hydroxytamoxifen (OHTam) were added respectively at different concentrations into the culture medium. Cell number and levels of ER mRNA were examined. RESULTS: The growth of CH3 cells became slower in estrogen-deprived medium than that in nomal culture and was higher in medium containing estrogen(E2) at concentration of 10^-8 mol/L than at concentration of 10^-6 mol/L. OHTam (10^-6mol/L) inhibited the growth of CH3 cell strain treated with E2. The expression of ER mRNA in CH3 cells was observed, the levels of ER mRNA in the E2 (10^-8mol/L) group, higher than those in estrogen deprived group. OHTam (10^-6mol/L) obviously inhibited the expression of ER mRNA. CONCLUSION: The growth of CH3 cells depends on estrogen, estrogen antagonists inhibits the growth of CH3 cells and decline the levels of ER mRNA. ER levels in human prolactinomas cell lines can be auto-regulated.     

A study on the mechanism of doxorubicin promoting IL-1β and collagen type I secretion in cardiac fibroblasts

AIM To observe the effect of adriamycin/doxorubicin （DOX） on the production of inflammatory cytokines and collagen in cardiac fibroblasts and its mechanism. METHODS Neonatal SD rat cardiac fibroblasts were isolated， cultured， and identified by immunofluorescence staining with monoclonal antibodies against vimentin observed under a confocal laser-scanning microscope. The Cell Counting Kit-8 assay was used to detect the toxicity of DOX on cardiac fibroblasts， and flow cytometry with annexin V-FITC/PI double staining was used to detect apoptosis. ELISA was used to detect the release of inflammatory factors in the supernatant of cultured cells. Immunofluorescence labeling assay was used to detected α-smooth muscle actin （α-SMA） expression and mitochondrial reactive oxygen species （mROS） in the cells. Western blot was used to detect the expression of nucleotide-binding oligomerization domain-like receptor protein 3 （NLRP3） inflammasome-related proteins in cardiac fibroblasts. RESULTS （1） Compared with the control group， DOX inhibited the proliferation of cardiac fibroblasts （P<0.05）， but had no significant effect on apoptosis （P>0.05）. （2） Treatment with DOX promotes the release of proinflammatory factors interleukin-1β （IL-1β） and IL-6 in cardiac fibroblasts （P<0.05）. （3） The expression of α-SMA， collagen type I and transforming growth factor-β in DOX treatment group increased significantly compared with control group （P<0.05）. （4） Compared with the control group， the levels of mROS， cellular NLRP3 and cleaved caspase-1 in cardiac fibroblasts increased significantly after DOX treatment. CONCLUSION Doxorubicin promotes cardiac fibroblasts to secrete IL-1β and collagen type I by promoting mROS production and activating NLRP3 inflammasome.     

辽宁省科学技术馆新馆园林景观设想

辽宁省科技馆新馆建成后,将成为辐射全省乃至东北的科普活动中心,将为全省各市科技馆的建设明确方向,将为全省人民,特别是青少年提供良好的科学学习场所,将在全国科技馆界产生重大影响。根据科技馆新馆景观环境和沈阳地区园林绿化发展现状进行分析,提出适合辽宁省科技馆园林景观可持续发展的设计方案。