Pathogen Analysis of Goat's Respiratory Diseases

To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.     

山东烟台市春季观赏植物景观特色与景观营造初探

以山东烟台市芝罘区"M"形状的市区分布为载体而插入山地的布局模式,具有先天优越的生态气候,选择这种先天优势的分布地区为研究地点,同步研究烟台市绿地系统规划,着重观测位于城市中心地带的滨海景观带、南山公园及其附属的大南山景区的春季观赏植物,利用其明确的代表性,从时空角度等方面深入探讨利用春季植物塑造景观的营造方法。     

IL-33/ST2 axis in systemic lupus erythematosus in relation to chronic kidney injury and disease activity

AIM: To elucidate the association between chronic kidney injury and interleukin-33 (IL-33; an alarmin)/suppression of tumorigencity 2 (ST2) in patients with systemic lupus erythematosus (SLE).METHODS: Serum levels of IL-33 and soluble ST2 (sST2) were assessed by ELISA in 50 SLE patients and 30 healthy controls (HC).RESULTS: The levels of IL-33 and sST2, and IL-33/sST2 ratio were significantly higher in SLE patients than those in the HC. The IL-33 and sST2 levels were positively associated with SLE disease activity index (SLEDAI), erythrocyte sedimentation rate (ESR), proteinuria and triglyceride, but negatively associated with complement C3. IL-33/sST2 ratio was positively associated with SLEDAI and estimated glomerular filtration rate (eGFR). Independent explanatory variables associated with high IL-33/sST2 included chronic kidney disease (CKD) staging and albumin (R²=0.442), especially CKD staging.CONCLUSION: Elevated serum sST2 and IL-33 levels in SLE patients are correlated with disease activity and risk factors of kidney injury. IL-33/sST2 ratio may serve as a potential biomarker for chronic kidney injury in SLE patients.     

鸡无尾性状的分子机制研究进展

动物的尾巴具有改变方向、控制升降、调整速度、支撑身体、防御、攻击、保温、示警、逃生和捕食等作用。鸟类的尾巴在飞行过程中起到平衡身体的关键作用，但在进化过程中尾部却出现了缩短和融合现象，如中国的瓢鸡及智利的Araucana鸡。作者简述了近年来关于鸡无尾性状的解剖学研究成果，发现无尾鸡在胚胎发育HH19期（Hamburger和Humilton标准分期）就已经出现无尾现象，而在结构上无尾鸡缺乏尾脂腺、尾羽、镰羽、尾椎骨和尾综骨。同时作者分析了导致尾部体节停止发育的分子遗传机制，包括周期性表达基因的转录调控影响尾部延伸过程、后端化因子梯度诱导尾部形成、Hox基因影响体节特化过程及基因突变有可能导致的无尾现象等，从而提出了可能导致家禽无尾性状的胚胎发育时期的关键分子及信号通路，包括Irx1和Irx2基因、Notch信号通路、Wnt信号通路、Fgf信号通路、RA信号通路等。研究鸡无尾性状的分子机制不仅可深入了解脊椎动物胚胎发育中尾部发育机制，更有利于揭示鸟类在进化过程中发生的尾部缩短及融合的机理。     

煤矸石栽培基质在农业中资源化利用研究现状

随着煤矸石在农业方面研究的不断深入,其作为栽培基质的开发和应用已成为重要的研究领域.该研究在梳理和分析国内外研究成果的基础上,综述了煤矸石理化及生物学特性、利用途径、已取得的主要成果及存在的问题,指出今后煤矸石在农业资源化利用中的发展趋势.     

气动式水稻株间机械除草装置研制

目的 针对水稻株间机械除草自动化程度低、难度大的问题，在机器视觉识别定位技术研究的基础上，研制一种气动式水稻株间机械除草装置。方法 采用机械设计理论、离散元动力学仿真方法结合田间试验，研制出气动式水稻株间机械除草装置。首先对气动式株间除草机构的结构进行设计，运用运动学方程计算并确定机构的几何参数，通过Pro/E运动学仿真验证机构的可行性；然后对除草刀齿与水田土壤的相互作用过程进行仿真，并对仿真结果进行验证试验；最后进行田间试验验证整机工作性能，并利用三因素五水平二次旋转正交试验对影响除草率与伤苗率的工作参数进行分析。结果 气动式水稻株间除草机构连杆长35.00 mm，摆杆长72.24 mm，除草部件到回转中心水平距离为84 mm，垂直距离为191 mm。离散元动力学仿真分析表明，倾角为10°的弯齿刀除草刀齿与土壤的接触阻力较小，阻力平均值为3.12 N，且对土壤的扰动程度较大，受影响的面积达149.69 cm²。田间试验中，在机具前进速度0.25 m/s、气缸伸缩速度0.45 m/s和除草深度2.5 cm的工作参数下，平均除草率为83.91%、伤苗率为3.63%。结论 该机具满足除草率大于80%、伤苗率小于4%的设计要求，能够满足水稻株间避苗除草的作业要求。     

冠菌素对玉米抗倒伏能力及产量的影响

在田间条件下，以玉米品种先玉335和郑单958为材料，在八展叶时于叶面喷施不同浓度 (0、0.01、0.1、1和10 μmol/L) 冠菌素 (COR)，研究了COR对玉米抗倒伏能力及产量的影响。结果表明:经10 μmol/L COR处理后，先玉335的株高和穗位高分别比对照降低了7.0%和19.9%，郑单958分别降低了20.8%和18.2%，单株叶面积、节间抗折断力、穗下第8~14节间长度和节间最大直径与对照间亦差异显著。且随着COR浓度的增加，先玉335和郑单958的株高、穗位高、单株叶面积和穗下第8~14 节间长度均逐渐降低；第8~14节间最大直径和节间抗折断力逐渐增大。经1 μmol/L COR处理后，2个供试玉米品种的产量、穗数、穗粒数和千粒重较对照均有所增加，其中先玉335的产量较对照增加了9.9%，郑单958增加了4.3%。综上所述，COR可以提高玉米的抗倒伏能力，不同品种玉米对COR的敏感性不同，其中先玉335抗倒伏的最适COR浓度为10 μmol/L，郑单958抗倒伏的最适COR浓度为1 μmol/L。