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81.
根据易感类个体对病毒的易感性不同把传统的易感类S分成n个子类Sk(k=1,2,…,n),建立了SnIRS传染病模型来研究易感性不同对疾病的影响,应用现代数学中的微分方程理论和非线性动力学的方法,得到了疾病传播的基本再生数及无病平衡点全局稳定性的阈值条件,证明了地方病平衡点的存在唯一性。 相似文献
82.
中9优547是湖北省黄冈市农科院用不育系中9A与恢复系R547配组育成的三系杂交早籼稻新组合,2010年通过湖北省农作物品种审定委员会审定。具有米质优、高产稳产、生育期适宜、制种产量高等优点,适宜于在稻瘟病、白叶枯病和纹枯病无病区或轻发区种植。 相似文献
83.
经用P~(32)每毫升25量μci的剂量标记松毛虫赤眼蜂,室内测定P~(32)放射性强度:50头成蜂为466~642cpm,20粒柞蚕卵为109—117cpm。P~(32)标记对成蜂寿命、繁殖力、性比均无不良影响。苹果园P~(32)标记放蜂表明:苹果小卷叶蛾的卵块寄生率达96%,即粒寄生率93.09%。在寄生卵块中有73.91%的卵块测出P~(32),平均每块的放射性强度为14.34cpm,说明是人工释放的效果。有26.09%的寄生卵块未测出P~(32)或很弱,此为自然赤眼蜂所寄生。 相似文献
84.
85.
在系统鉴定、药敏试验和血甭型鉴定的基础上,对52株致病性大肠杆菌和4株标准血清型大肠杆菌进行了质粒DNA分析,结果表明,菌株的质粒得率为100%,来源相同的菌株具有相同或相似的质粒图谱,来源不同的菌株具有不同的质粒图谱,质粒DNA分析为四川规模化猪场致病性E.coli的分子流行病学调查提供了科学依据。质粒图谱与血清型及耐药谱之间无明显关系。 相似文献
86.
陆地棉对黄萎病抗性的分子标记研究 总被引:14,自引:0,他引:14
利用陆地棉标准系TM-1和常抗棉2个陆地棉品种杂交并自交,获得109个F2单株及F2:3家系为作图群体,以SSR、RAPD和SRAP 3种分子标记进行抗黄萎病性状的分子标记筛选。结果从1611对(条)引物中仅筛选到70对(条)多态性引物,获得75个多态性位点并进行标记间的连锁性分析。75个标记构建了一个包括15个连锁群,全长535 cM的陆地棉品种间分子标记遗传连锁图,标记间平均距离为11.15 cM,有27个标记不能进入任何连锁群。连锁群的标记数最少2个,最多6个;长度从1.0 cM到92.7 cM不等。对其F2:3家系的成株期抗黄萎病性状即平均病情指数的分布进行分析,显示其呈正态分布,进一步说明陆地棉对黄萎病的抗性为数量遗传;单标记分析及复合区间作图,检测出与抗黄萎病性相关的3个QTL,分别位于第3、5、6连锁群上,贡献率分别为14.15%、3.45%和18.78%。另外,对该群体生长过程中黄萎病不同发病高峰期的病情也进行了分析。 相似文献
87.
进行了安普霉素耐药大肠杆菌耐药表型的研究.采用常规方法和生化鉴定管对有过安普霉素用药史的鸡场分离的鸡源病原性大肠杆菌进行鉴定,并用试管二倍稀释法测定其最低抑菌浓度(MIC),筛选出鸡源安普霉素耐药大肠杆菌;采用药敏纸片法研究了这些耐药菌对安普霉素等14种抗菌药物的敏感性.共筛选出7株对安普霉素耐药的鸡源性大肠杆菌,这些耐药菌株全部对安普霉素、妥布霉素、奈啶酸、多西环素和阿莫西林耐药;大部分对庆大霉素、链霉素、卡那霉素、壮观霉素也呈现耐药.对新霉素的耐药较低,对阿米卡星高度敏感.部分交叉耐药现象的存在揭示对安普霉素等氨基糖苷类药物产生耐药性的菌株,其他抗生素也可能对它们失去疗效. 相似文献
88.
Effects of homocysteine on number and activity of endothelial progenitor cells from peripheral blood
WANG Xing-xiang SHANG Yun-peng ZHU Jun-hui CHEN Jun-zhu ZHU Jian-hua TAO Qian-min GUO Xiao-gang WANG Zhan-kun ZHANG Li 《园艺学报》2005,21(7):1274-1278
AIM: To investigate whether homocysteine (Hcy) has influences on endothelial progenitor cell (EPCs) number and activity from peripheral blood. METHODS: Total mononuclear cells (MNCs) were plated on fibronectin-coated culture dishes and cultured for 7 days, and then attached cells were stimulated with Hcy or vehicle control for 6 h, 12 h, 24 h and 48 h. The adhesion, proliferation, migration and in vitro vasculogenesis activity of EPCs were assayed, respectively. RESULTS: Incubation of isolated human MNCs with Hcy dose and time-dependently decreased the number of EPCs with maximum at 200 μmol/L for 24 hours (35.7±6.7 vs 62.5±10.6, P<0.01). In addition, Hcy impaired EPC proliferative (0.531±0.061 vs 0.328±0.055, P<0.05), migratory (26.3±6.4 vs 6.4±3.7, P<0.01), adhesive (33.1±8.1 vs 17.4±7.5, P<0.01) and vasculogenesis capacity (25.4±9.1 vs 10.4±4.7, P<0.01) in a dose and time-dependent manner. CONCLUSION: It is suggested that Hcy may result in the reduction of EPCs and decrease EPC functional activity. 相似文献
89.
AIM: To down-regulate expression of c-myc through antisense therapy and to investigate its effect on the sensitivity of osteosarcoma MG-63 cells to cisplatin-induced apoptosis. METHODS: The recombinant adenovirus (Ad-Asc-myc) encoding antisense c-myc fragment was constructed and transfected into osteosarcoma MG-63 cells in vitro in order to down-regulate the expression of c-myc, and the change in the sensitivity to cisplatin-induced apoptosis was observed. MTT, Western blot, RT-PCR, flow cytometry (FCM) and electron microscope were used to evaluate tumor cell proliferation in vitro, genes expression related to apoptosis regulation and effects on the sensitivity of osteosarcoma MG-63 cells to cisplatin-induced apoptosis. RESULTS: Ad-Asc-myc down-regulated the expression of c-myc protein after transfected MG-63 cells for 48 h, combined with the treatment of 2.0 mg/L cisplatin for 2 h inhibited tumor cell proliferation in vitro by 38.0%. RT-PCR revealed that Ad-Asc-myc down-regulated the expression of Bcl-2 and up-regulated the expression of Bax. No appreciable change was observed in the expression of E2F-1. FCM showed that Ad-Asc-myc induced apoptosis in intransfected cells, and rendered it more sensitive to cisplatin. CONCLUSION: Antisense c-myc is able per se to induce apoptosis and sensitize osteosarcoma cells to cisplatin-induced apoptosis. 相似文献
90.
AIM: In this study, we investigated the anticancer effect and mechanisms of manumycin on pancreatic cancer cell line-Panc-1 and the role of p38MAPK pathway in apoptosis. METHODS: The test of anticancer effect was performed by MTT assay. Apoptosis was induced in the cells by manumycin and then treated with SB203580, a specific p38MAPK inhibitor. A quantitative caspase-3 activity assay kit was used in this experiment. RESULTS: Manumycin (6 μmol/L, 18 μmol/L, 54 μmol/L) significantly inhibited cell growth of pancreatic cancer cell line Panc-1. The inhibition rates 24 h after treatment with 6 μmol/L, 18 μmol/L and 54 μmol/L manumycin were 8.9%, 21.9% and 67.0%, respectively. Compared with the control group, the survival levels of the last two groups were of significant statistical difference (P<0.01). The anticancer effects also showed dosage-effect relationship, the value of IC50 24 h after treatment was 34.7 μmol/L. In addition, this reagent simultaneously activated caspase-3 protein, which was partly blocked by p38MAPK specific inhibitor, SB203580. CONCLUSION: Manumycin exerted anticancer effect on Panc-1 cell line via inducing cell apoptosis, which was partly regulated by p38MAPK. 相似文献