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111.
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JIANG An-li KANG Lu-dong ZHAO Chun-hua BI Wen-xiang HU Xiao-yan KONG Feng XU Song-de 《园艺学报》2003,19(1):55-58
AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor. 相似文献
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水稻白叶枯病菌对噻枯唑的抗药性监测 总被引:11,自引:0,他引:11
采用活体寄主法和离体含药平板法 ,测定了 1999年安徽省滁州市田间水稻白叶枯病菌对噻枯唑的抗药性。结果表明 ,在水稻活体上存在噻枯唑抗药突变体 ,其比例为67.65%,比1997、1998年有较大程度升高 ,但是离体含药平板上未能监测到抗药突变体。 相似文献
115.
十种常用农药与球孢白僵菌的生物学相容性 总被引:24,自引:2,他引:24
球孢白僵菌孢子粉与10种常用农药相容性的测定结果显示,随着孢子浓度上升,所试农药对孢子的抑制作用均有不同程度的增强。在1/10田间常规使用浓度下,百菌清和代森锰锌均能抑制或杀死孢子(萌发率<1%)。除阿维菌素外,所有杀虫剂均与白僵菌孢子相容,在常规使用浓度的10倍稀释液中孢子萌发率达90%以上。吡虫啉、蚜虱灵、灭多威和氟虫腈与孢子的相容性最好,其中吡虫啉和蚜虱灵对孢子萌发率的影响不明显随药剂浓度的变化而变化,即使在田间常规使用浓度下孢子萌发率也在95%以上,而阿维菌素与白僵菌的相容性极差。因此,应用白僵菌制剂防治害虫,选择生物学相容性好的农药以低剂量与白僵菌制剂混用,既可使菌剂增效,又可大幅度降低化学药剂用量。 相似文献
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β- 氨基丁酸诱导甜( 辣) 椒抗疫病作用的研究 总被引:12,自引:2,他引:12
报道了用β- 氨基丁酸( DL-β-aminon-butyric acid, BABA) 喷雾处理辣椒叶片和茎后的诱导抗疫病作用。研究证明: 高浓度BABA ( 1 000 g/mL) 对离体辣椒疫霉病菌无抗菌活性, 用其喷雾处理辣椒的茎叶所诱导的抗疫病作用可完全控制其危害; 用BABA 诱导处理后3 d 接种辣椒疫霉病菌, 辣椒植株开始表达出较高的诱导抗性, 这种抗病作用可持续20 d 以上, 并表现出与数量抗病性相似的特性。 相似文献
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AIM:To investigate the effect of the heat shock response on the reperfusion arrhythmias(RAs) and the possible mechanism involved. METHODS:Fifty-five Sprague Dawley rats were randomly divided into 2 groups: the heat shock group (group H,n=29) and the control group (group C,n=26). The rats in group H were preconditioned with heat shock 24 hours before, and that in group C were not. The hearts of 16 rats in group H and 16 in group C were exercised and mounted on a non-circulating Langendorff perfusion apparatus and perfused retrogradely with modified K-H buffer and mimic ischemia/reperfusion was applied. Additionally, conventional intracellular microelectrode techniques were used for recording such electrophysiological parameters as resting potential(RP), action potential amplitude(APA), over shot(OS), maximum depolarization velocity(Vmax) of the hearts of other 13 rats in group H and 10 in group C. RESULTS:①Prior heat stress significantly decreased reperfusion arrhythmia. ②The amount of CK release in the effluent in group H was much less than that in group C. ③Myocardial HSP70 content was elevated significantly in group H. ④Heat stress significantly increased myocardial anti-oxydases activity and decreased lipid peroxydative products. Additionally, heat stress significantly reduced the Vmax of action potential. It indicated that rapid Na+ channel of papillary muscles may be inhibited by heat shock. The degree of change of Vmax after ischemia in H group was significantly less than that in group C. And the time of reperfusoin with Tyrode's solution till the action potential appeared as large as that before perfusion with mimic ischemic solution is shorter in group H than in group C. CONCLUSION:Heat shock pretreatment markedly reduces ischemia/reperfusion-induced injury of heart and ventricular arrhythmias in rats and this effect may be associated with the inhibition of rapid Na+ channel of papillary muscles by heat shock and the increase in myocardial HSP70 and anti-oxydase activity. 相似文献
120.
AIM:To examine the effects of recombinant human hepatocyte growth factor(rhHGF) and native calf HGF(cHGF) on SMMC-7721 human hepatocellular carcinoma(HCC) cell line. METHODS:Human HCC cell line culture, photometric assay, and flow cytometric assay were used in this study .RESULTS:A similar type of dose-dependent cell growth inhibition effect on SMMC-7721 human HCC cells by rhHGF(5-20 μg/L) as well as by cHGF(25-100 mg/L) had been found, with the maximal effect at the highest concentration used. Approximately over 50% of the cells treated with rhHGF(5 μg/L, 10 μg/L, 20 μg/L) accumulated in the quiescent G0/G1 phase of the cell cycle over incubation periods for 3 d. CONCLUSION:The growth of SMMC-7721 human HCC cells was strongly inhibited by both rhHGF and cHGF. This might be because the cells exposed to HGF became arrested in the G0/G1 phase. 相似文献