全文获取类型
收费全文 | 437篇 |
免费 | 50篇 |
专业分类
林业 | 13篇 |
农学 | 28篇 |
基础科学 | 2篇 |
104篇 | |
综合类 | 17篇 |
农作物 | 22篇 |
水产渔业 | 44篇 |
畜牧兽医 | 207篇 |
园艺 | 12篇 |
植物保护 | 38篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 8篇 |
2020年 | 14篇 |
2019年 | 18篇 |
2018年 | 26篇 |
2017年 | 8篇 |
2016年 | 16篇 |
2015年 | 22篇 |
2014年 | 13篇 |
2013年 | 46篇 |
2012年 | 10篇 |
2011年 | 22篇 |
2010年 | 22篇 |
2009年 | 12篇 |
2008年 | 35篇 |
2007年 | 17篇 |
2006年 | 29篇 |
2005年 | 18篇 |
2004年 | 20篇 |
2003年 | 14篇 |
2002年 | 16篇 |
2001年 | 13篇 |
2000年 | 13篇 |
1999年 | 14篇 |
1998年 | 6篇 |
1997年 | 7篇 |
1996年 | 5篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1977年 | 1篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1973年 | 3篇 |
1972年 | 2篇 |
排序方式: 共有487条查询结果,搜索用时 78 毫秒
21.
Eduardo Segundo Dietrich E. Lesemann Germán Martín María P. Carmona Leticia Ruiz Isabel M. Cuadrado Leonardo Velasco Dirk Janssen 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(1):81-87
Amaranthus leaf mottle virus (AmLMV) was classified as a member of the genus Potyvirus on the basis of its particle morphology, serology, and biological properties (Casetta et al., 1986). Based on these properties, an Amaranthus viridis-infecting virus isolated in Spain, causing mottle and leaf blistering as well as reduced growth has been identified as AmLMV.
The 3′ terminal genomic region of this and a reference isolate from Italy has been sequenced and reveals a 95% nucleotide
identity between the two isolates. The sequenced part comprises the coat protein with 281 amino acids and 315 nucleotides
of the 3′ untranslated region (UTR) preceding a polyadenylated tail. Pairwise comparisons and phylogenetic analysis of the
nucleotide and deduced amino acid sequences of the CP and 3′ UTR of the cloned cDNAs with those of other potyviruses shows
that AmLMV is a distinct potyvirus closely related to Potato virus Y. 相似文献
22.
AFLP profiles were generated from poplar leaf rust ( Melampsora larici-populina ) collected from Populus trichocarpa cv. Trichobel at six sites in the UK in 2001. Of a total of 145 isolates, 121 were unique AFLP phenotypes. On one occasion, the same AFLP phenotype was found at two sites. The analysis indicated that 81·88% of the total variation was attributable to differences among individuals within replicate plots, 3·47% to differences among plots within sites and 14·65% to differences among sites. Weir & Cockerham's θ was calculated as 0·138 and θ B , the Bayesian analogue of F ST , as 0·146. Overall, there was sufficient gene flow among different sites to prevent genetic drift, as Nm was calculated as 1·562 using θ and as 1·462 using θ B . Nei's genetic distance and population-assignment test indicated that the rust from a site in north-east England was differentiated from those from other sites. There was no significant correlation between genetic and geographical distances. The results indicate that sexual reproduction has a major influence on the relative lack of population structure of M. larici-populina in the UK. 相似文献
23.
24.
Objective To evaluate standing, percutaneous, ultrasound-guided, transthoracic liver biopsy in mares, and transabdominal, laparoscopically-guided, liver biopsy under general anaesthesia in foals, as techniques for obtaining tissue for assessment of copper status. The techniques were evaluated with respect to ease of use and effect on the animal.
Procedure Twenty of 24 Thoroughbred mares and 21 of their foals were biopsied. The animals were part of a larger study of the effect of copper supplementation on copper status and the prevalence of developmental orthopaedic disease. Livers were also collected from unrelated horses and sampled to investigate the variability in the distribution of copper in liver tissue.
Result The biopsy technique caused no lasting effect on the mares, but there was an increased risk of viscus penetration associated with taking multiple biopsy cores. The use of ultrasonography to scan the target area for the liver identified four cases that were not appropriate candidates for liver biopsy, because of large intestine being located in the biopsy area. In the foals there were no serious postoperative adverse effects, nor was there any evidence of problems caused by the procedure when the abdomen was examined post-mortem at 5 months of age. In livers collected to investigate the variability of copper concentration, copper appeared to be relatively evenly distributed through the liver.
Conclusion Standing, percutaneous, ultrasound-guided, transthoracic liver biopsy in mares, and transabdominal, laparoscopically-guided, liver biopsy under general anaesthesia in foals are convenient procedures for obtaining liver tissue for assessing copper status in horses. The use of ultrasound to identify liver tissue is recommended, especially in older mares. 相似文献
Procedure Twenty of 24 Thoroughbred mares and 21 of their foals were biopsied. The animals were part of a larger study of the effect of copper supplementation on copper status and the prevalence of developmental orthopaedic disease. Livers were also collected from unrelated horses and sampled to investigate the variability in the distribution of copper in liver tissue.
Result The biopsy technique caused no lasting effect on the mares, but there was an increased risk of viscus penetration associated with taking multiple biopsy cores. The use of ultrasonography to scan the target area for the liver identified four cases that were not appropriate candidates for liver biopsy, because of large intestine being located in the biopsy area. In the foals there were no serious postoperative adverse effects, nor was there any evidence of problems caused by the procedure when the abdomen was examined post-mortem at 5 months of age. In livers collected to investigate the variability of copper concentration, copper appeared to be relatively evenly distributed through the liver.
Conclusion Standing, percutaneous, ultrasound-guided, transthoracic liver biopsy in mares, and transabdominal, laparoscopically-guided, liver biopsy under general anaesthesia in foals are convenient procedures for obtaining liver tissue for assessing copper status in horses. The use of ultrasound to identify liver tissue is recommended, especially in older mares. 相似文献
25.
This study aimed at capturing the spatial variability of landscape patterns and their trajectories of change from 1950 to
2000 within a watershed, which is representative of areas of intensive agricultural use. After an analysis of landscape features
changes for the entire watershed based on aerial photographs, hierarchical clustering analysis provided a typology of landscape
patterns for the cadastral lots. Following that, the trajectory of change of each lot was characterized (nature, importance,
direction, rate of change). Seven types of landscape patterns are distinguished by the relative importance of different classes
of landscape features and 51 trajectories of change were identified for the lots. The analysis shows that although the majority
of lots were subjected to a homogenization of their landscape patterns since 1950, this trend is not entirely uniform and
that since 2000 it occurs alongside trends towards diversification of certain landscape features on some lots. Furthermore,
nearly a third of the lots are not following the main trajectories of change detected. Thus, the results suggest that extrinsic
forces (policies, technologies) that are directing main changes in areas of intensive agricultural use toward uniformity could
be modulated by internal forces (uses and values of the population). A better understanding of theses internal forces seems
crucial to manage landscapes. From a methodology standpoint, although the hierarchical clustering analyses appear useful for
understanding the spatial and temporal variability of landscape patterns, particular attention must be given to validating
the typology chosen to characterize them. 相似文献
26.
Abecia José-Alfonso Barrio Antonio Ruiz José-Antonio 《Veterinary research communications》2022,46(2):609-612
Veterinary Research Communications - Twenty-five ewe-lambs born in November were used to measure the effect of the presence of cyclic or non-cyclic adult ewes on the age at first fertile estrus.... 相似文献
27.
Citrus response to salinity: growth and nutrient uptake 总被引:1,自引:0,他引:1
To determine the effects of salinity on relative growth rate (RGR), net assimilation rate on a leaf weight basis (NAR(w)), leaf weight ratio (LWR), and nutrient uptake and utilization of citrus, we grew four citrus rootstocks (sour orange, Cleopatra mandarin, Carrizo citrange and Citrus macrophylla) in nutrient solutions containing 0, 10, 20, 40 or 80 mM NaCl for 20, 40 or 60 days. For each element analyzed, specific absorption rate (SAR) and specific utilization rate on a leaf basis (SUR(L)) were calculated for the period between Days 40 and 60. Relative growth rate decreased with time for all treatments and rootstocks. Salt treatment significantly reduced both RGR and NAR(w), whereas LWR showed no definite trend. In all rootstocks, NAR(w), but not LWR, was significantly correlated with RGR, indicating that NAR(w) was an important factor underlying the salinity-induced differences in RGR among the citrus rootstocks. At Day 60, salinity had a significant effect on leaf concentrations of Cl, Na, K, Ca, Mg, P, Fe, Mn and Zn and on the SAR and SUR(L) of most elements. In general, RGR was correlated with SAR and SUR(L). Therefore, in addition to osmotic effects and the inhibitory effects of high concentrations of Cl(-) and Na(+), an imbalance of essential nutrients may also contribute to the reduction in plant growth under saline conditions. 相似文献
28.
M. Ugarte‐Ruiz R. A. Stabler L. Domínguez M. C. Porrero B. W. Wren N. Dorrell O. Gundogdu 《Zoonoses and public health》2015,62(7):497-500
Infections from Campylobacter jejuni pose a serious public health problem and are now considered the leading cause of foodborne bacterial gastroenteritis throughout the world. Sequencing of C. jejuni genomes has previously allowed a number of loci to be identified, which encode virulence factors that aid survival and pathogenicity. Recently, a Type VI secretion system (T6SS) consisting of 13 conserved genes was described in C. jejuni strains and recognised to promote pathogenicity and adaptation to the environment. In this study, we determined the presence of this T6SS in 63 Spanish C. jejuni isolates from the food chain and urban effluents using whole‐genome sequencing. Our findings demonstrated that nine (14%) strains harboured the 13 ORFs found in prototype strain C. jejuni 108. Further studies will be necessary to determine the prevalence and importance of T6SS‐positive C. jejuni strains. 相似文献
29.
Bird Michael G. Hardner Craig M. Dieters Mark Heberling Mónica Montouto Cristian Arnold Roger J. Ruiz Federico Schapovaloff Juan Gore Peter L. 《New Forests》2022,53(1):101-123
New Forests - Eucalyptus dunnii Maiden (Dunn’s white gum) is a fast-growing tall tree with a restricted natural distribution in south-east Australia and favorable wood properties for pulp... 相似文献
30.
Georgina Limon Elisa G. Lewis Yu-Mei Chang Hugo Ruiz Maria Elba Balanza Javier Guitian 《Preventive veterinary medicine》2014
Livestock disease surveillance is particularly challenging in resource-scarce settings, where disease events are often unreported. Surveillance performance is determined as much by the quantifiable biological attributes of the disease, as it is by motivations and barriers perceived by livestock keepers for disease reporting. Mixed methods designs, which integrate the collection, analysis and interpretation of qualitative and quantitative data in a single study, are increasingly used across different disciplines. These designs allow for a deeper exploration of the topic under investigation, than can be achieved by either approach alone. 相似文献