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51.
Hematogenous metastasis requires the arrest and extravasation of blood-borne tumor cells, possibly involving direct adhesive interactions with vascular endothelium. Cytokine activation of cultured human endothelium increases adhesion of melanoma and carcinoma cell lines. An inducible 110-kD endothelial cell surface glycoprotein, designated INCAM-110, appears to mediate adhesion of melanoma cells. In addition, an inducible endothelial receptor for neutrophils, ELAM-1, supports the adhesion of a human colon carcinoma cell line. Thus, activation of vascular endothelium in vivo that results in increased expression of INCAM-110 and ELAM-1 may promote tumor cell adhesion and affect the incidence and distribution of metastases. 相似文献
52.
Vitamin E: function and effects of deficiency 总被引:1,自引:0,他引:1
53.
Induction of bulbing of tulip shoots in vitro 总被引:2,自引:0,他引:2
Bulbing has been induced in adventitious shoots from floral stem explants of Tulipa ‘Merry Widow’. Incubation of 1-mm thick explants at 20°C on a medium containing 1 mg l−1 NAA and BAP for 14–18 weeks, followed by 4°C for 8 weeks, produced a consistent bulbing response in shoots after transfer back to 20°C. Bulbing was further enhanced by transfer to 25°C and by raising the concentration of sucrose in the medium. Microscopy indicated the presence of developing meristematic centres in shoots 12 weeks of age. Shoots over 16 weeks of age containing meristematic centres gave a variable bulbing response with applied gibberellins and cold incubation, depending on whether gibberellins were supplied continuously in the medium or via a brief soak in sterile gibberellin solutions before sub-culturing. A GA3 “soak” of 1.0 mg l−1 was optimum for bulb production. The possible mechanisms of cold and gibberellin effects are discussed. 相似文献
54.
Use of sodium hydroxide treated selenium deficient barley to induce vitamin E and selenium deficiency in yearling cattle 总被引:1,自引:0,他引:1
Selenium deficient barley grown in Northern Ireland was treated with sodium hydroxide to deplete it of vitamin E. Housed cattle fed a complete diet based on this treated barley developed nutritional degenerative myopathy, showing that spontaneous myopathy in yearling cattle can be the result of vitamin E and selenium deficiency alone. The diet used is as effective and cheaper than others presently in use for inducing degenerative myopathy. 相似文献
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56.
Christine E. Rice C. L'Ecuyer Mohendra Merriman 《Canadian journal of veterinary research》1968,32(3):468-473
As shown by density gradient ultracentrifugation and column chromatography, pigs formed IgM antibodies during the first week following vaccination with Brucella abortus, strain 19. At this time their sera reacted in both plate and tube agglutination but not in complement-fixation tests. A few days later, when IgG antibodies had developed, agglutination titers were still high and some activity was recorded in hemolytic complement-fixation tests. A similar sequence was observed in pigs repeatedly inoculated with phenol-killed suspensions of B. abortus. As the proportion of IgM to IgG antibodies decreased, agglutinin titers fell in relation to complement-fixing titers. In some animals the conglutinating complement absorption test became positive earlier than the plate agglutination. 相似文献
57.
Studies of Johne's Disease in Canada: XII. Electrophoretic Analysis of Changes in Serum Proteins in Infected Cattle and Sheep
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Delma Cochrane Christine E. Rice J. Carrière 《Canadian journal of veterinary research》1965,29(8):209-213
Electrophoretic examinations were made on sera collected monthly for a period of eleven months from ten cattle naturally or experimentally infected with Johne''s bacilli and from ten contact sheep. With one exception, the percentage estimates for the four major classes of serum proteins, albumin, alpha1-, alpha2-, beta- and gamma-globulins did not differ significantly in sera from infected and non-infected, presumably healthy cattle. One cow with a persistently high complement-fixing titre with Johne''s bacillus antigen, showed an exceptionally high proportion of gamma-globulin in its serum. The percentage of gamma-globulin tended to be higher in sera of contact sheep than in that of normal sheep sera but the monthly variation in the relative proportion of these and other globulins showed no evident relationship to the fluctuations observed in the specific complement-fixing and anti-complementary properties of these sera. 相似文献
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60.
The Effect of Unheated Normal Bovine Serum on the Complement-Fixing Activity of Heat Inactivated Bovine Antiserum with Homologous Antigen: III. Immunodiffusion Studies
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Christine E. Rice 《Canadian journal of veterinary research》1962,26(9):212-217
Examination by immuno-diffusion methods in agar gel plates demonstrated that the supplementing fraction precipitated from normal bovine serum by dialysis against dilute buffer, pH 5.0 to 6.6, contains at least three main antigens, and a weaker fourth. Two of these antigens or antigen determinants appeared to be present in globulin fractions prepared by dilution with distilled water or differential precipitation with ammonium or sodium sulphate. 相似文献