柑橘溃疡病菌LAMP实时浊度检测方法的建立及应用

以柑橘溃疡病菌特异性高的假定蛋白基因（登录号：AE008923.1）为靶标，设计并筛选4条引物来特异性识别靶标序列中的6个独立区域，建立柑橘溃疡病菌LAMP实时浊度检测方法。该方法利用实时浊度仪检测反应过程中产生的焦磷酸镁白色沉淀，实现对LAMP整个反应过程的实时监控。从镁离子浓度、甜菜碱浓度、dNTPs浓度、引物浓度和反应温度5个变量参数对 LAMP检测体系进行优化，并对方法的特异性、灵敏度、稳定性及实际样品检测效果进行评价。结果显示，该体系经优化后稳定性良好，可在66 ℃ 恒温条件下，60 min内完成检测；DNA检测下限可达0.02 ng/μL，灵敏度与荧光定量PCR方法相同，比常规PCR方法高1个数量级；能快速、准确地对田间疑似样品进行检测，与荧光定量PCR方法的检出情况一致，没有漏检。结果说明柑橘溃疡病菌实时浊度LAMP检测方法操作简便、所需时间短，特异性与灵敏度高，为柑橘溃疡病菌的快速筛查提供较好的途径。     

大白菜花青素含量及色差指标相关性研究

为了探寻大白菜花青素含量快速测定的方法,本文利用pH差计法和色差仪分别测定了‘城阳青’(普通大白菜)、‘秋宝黄’(黄心白菜)和‘彩凤’3个不同叶色大白菜品种花青素含量以及色差指标,并对6个色泽参数与花青素含量进行了相关和回归分析。结果表明:6个色泽参数均能反映白菜叶片间叶色的细微变化,‘城阳青’和‘秋宝黄’不含花青素,紫色白菜‘彩凤’的花青素主要分布于由外向内的第1～4层叶片。色泽参数L,b,h和C分别与花青素含量呈极显著或显著负相关关系(相关系数分别为-0.85**,-0.58**,-0.49*,-0.58**)。以色泽参数值L、a、h与花青素含量建立了多元回归方程:y=90.24-1.07x1+5.65x2-142.71x3(x1为L值,x2为a值,x3为h值,y为花青素含量),该回归方程相关系数为0.9629。通过色差计依据建立的回归方程可快速测定紫色大白菜花青素的含量。     

Effect of Dietary Lactobacillus fermentum from Pig on Growth Performance,Nutrient Apparent Digestibility,Faecal Microflora Number and Serum Immune Indices in Weaned Piglets

This experiment was conducted to study the effects of Lactobacillus fermentum on growth performance, nutrient apparent digestibility, faecal microflora number and serum immune indices of weaned piglets.A total of 160 weaned piglets with an average age of (30±2) days and averge body weight of (7.85±0.68) kg were randomly distributed into 4 groups with 4 replicates per group and 10 piglets per replicate feeding basal diet, 0.1%, 0.2%, and 0.3% Lactobacillus fermentum supplementation diets, respectively.The experiment lasted for 35 d.The results showed that, compared with the control group, the average daily gain, the feed conversion ratio, the apparent digestibility of crude protein, calcium and total phosphorusin of Lactobacillus fermentum groups were all increased in varying degrees.The optimum supplemental level of Lactobacillus fermentum should be 0.2%, and at this level, the average daily gain was significantly increased by 8.70% (P<0.05), the F/G was decreased by 4.62% (P<0.05), the apparent digestibility of crude protein, calcium and total phosphorus were significantly increased (P<0.05), the number of faecal E.coli was significantly decreased (P<0.05), and the serum IgG was significantly increased by 8.39% (P<0.05).The results indicated that Lactobacillus fermentum supplementation could improve the immune function, growth performance, the apparent digestibility of crude protein, calcium and total phosphorus and reduce the number of faecal E.coli of weaned piglets.     

黄瓜根际解钾细菌的分离筛选、鉴定及其促生效果

以黄瓜根际土壤为试材,采用亚历山大硅酸盐细菌培养基从黄瓜根际土壤中分离筛选解钾菌,用原子吸收法测定菌株的解钾能力,选出解钾能力较强的菌株进行菌种鉴定,同时研究其对黄瓜幼苗生长的影响。结果表明:从黄瓜根际土壤中共分离获得9株细菌,其中PK1、PK3和PK7具有较强的解钾能力,液体培养96h后其解钾率分别为30.05%、33.78%和44.01%。依据菌株的形态学特征和生理生化特征鉴定,PK1和PK3为恶臭假单胞菌(Pseudomones putida),PK7为胶质类芽孢杆菌(Paenibacillus mucilaginosus)。盆栽试验结果表明,与对照相比,14d后PK1提高黄瓜的根长、株高、鲜质量和干质量分别为42.60%、10.45%、48.65%、33.01%,PK3分别对应提高65.26%、21.09%、83.78%和49.48%,PK7分别对应提高79.46%、44.26%、105.41%和113.03%。     

日光温室基质袋培番茄灌溉监控系统设计

为了解决日光温室基质袋培番茄精准灌溉的问题,设计了日光温室基质袋培番茄灌溉监控系统。系统利用数据采集器获取日光温室相关的环境因子,结合番茄生长和水分吸收模型计算灌溉量,并通过灌溉控制器控制电磁阀开闭时间的长短进行灌溉。实验结果表明系统稳定可靠,与传统的人工灌溉模式相比,使番茄增产17.9%,节水35.4%,因此适合用于日光温室基质袋培番茄的精准灌溉。     

Effects of CENP-W down-regulation on human glioma U87 cells

AIM: To study the effect of centromere protein W (CENP-W) down-regulation on human glioma U87 cells.METHODS: Small interfering RNA (siRNA) was used to inhibit the expression of CENP-W in the U87 cells. The interference effect of siRNA was evaluated by RT-qPCR and Western blot. The proliferation of the cells was analyzed by MTT assay, BrdU staining and colony formation experiment. Transwell chamber assay was used to detect the invasion ability of the cells. The cell migration ability was measured by a scratch test. The changes of the cell cycle distribution and apoptosis were analyzed by flow cytometry.RESULTS: The results of MTT assay, colony formation experiment and BrdU staining showed that the cell proliferation and colony formation abilities in experimental group were significantly lower than those in control group and negative control group. The results of Transwell and scratch experiments showed that the migration and invasion abilities in experimental group were weaker than those in blank control group and negative control group. The results of flow cytometry analysis showed that the cell cycle distribution in experimental group was arrested in G₀/G₁ phase. The percentage of apoptotic cells in experimental group was higher than that in control group (P<0.05).CONCLUSION: Down-regulation of CENP-W expression inhibits the proliferation, migration and invasion of human glioma cells and promotes the apoptosis of the cells, suggesting that CENP-W may be a potential target of gene therapy for human glioma.     

丹参对奶牛脂肪变性肝细胞NF-κB和CYP450表达的影响

为探讨丹参对脂肪变性奶牛肝细胞的作用机制,采用胶原酶两步灌流法分离培养奶牛肝细胞,不同浓度DL-乙硫氨酸处理,油红染色观察其脂滴数量,MTT法测定肝细胞的活性,生化法检测TG含量。用不同浓度的丹参处理变性肝细胞后,生化法检测ALT、AST、TG、SOD、MDA的含量或活性,ELISA法测定TNF-α的含量,Western blot法检测NF-κB和CYP450的蛋白表达水平。结果显示,筛选出2 mmol/L的DL-乙硫氨酸作用24 h作为体外造模的最佳条件。用丹参处理后,能降低ALT、AST、TG和MDA的活性或含量,抑制TNF-α的释放,增加SOD活性。随丹参浓度增加和处理时间的延长,CYP450的表达呈逐渐降低的趋势。24 h时,丹参对NF-κB的表达无明显影响;48 h时,随丹参浓度的增加,NF-κB的表达呈逐渐降低的趋势。这表明丹参对奶牛脂肪肝有一定的治疗作用,其作用机制与促进脂质代谢、抑制肿瘤细胞坏死因子、抗脂质过氧化及降低NF-κB和CYP450的表达密切相关。     

杨树品系对杨干象抗性研究初探

杨树不同品系对杨干象抗性差异很大,以美洲黑杨为亲本的多数外来品种对杨干象抗性差,也使杨干象种群迅速扩大。该文根据调查的3年生和4年生连续2 a的不同杨树品系的平均虫口密度,根据聚类分析的方法将其划分为高感虫品系、感虫品系、低抗虫品系、中抗虫品系和高抗虫品系5个级别,结合杨树品系的类型、形态特征等进行分析,为生产上选用杨树抗性品系和减少杨干象大发生的危害及抗虫育种等方面提供参考。     

长沙市苗木基地土壤肥力的调查与评价

为了对长沙市苗木基地土壤肥力进行调查,选取了长沙市7个代表性苗木基地,研究和分析了各基地的土壤物理、化学性状,并采用Fuzzy综合评价对各基地的土壤肥力综合水平进行了评价.结果表明,长沙市苗木基地土壤pH值偏低,容重较大,土壤质地粘重,土壤有机质和氮素的含量中等偏低,土壤磷素和钾素的含量则较丰富；除林业基地外,其他基地土壤肥力综合指标均处于中等偏上水平.     

辽西地区五个冬桃新品种抗旱性差异

以辽西朝阳地区5个冬桃新品种为试材,分析了各品种冬桃的叶片保水力、水分亏缺状况、水分利用效率等指标,比较不同品种冬桃的抗旱能力,以期为进一步筛选适宜当地的冬桃栽培品系提供参考依据。结果表明:各冬桃品系在抗旱特性方面的综合表现为:"傲雪4号"表现最好,以下依次为"傲雪3号""傲雪5号""傲雪6号""傲雪2号"。