Change of reactive oxygen species production in co-culture model of rat astrocytes and motor neurons VSC 4.1

AIM: To explore the mutual effect of co-culture of wild-type astrocytes (ASC) and motor neurons VSC4.1 (VSC) on the respective ability to produce reactive oxygen species (ROS). METHODS: The inhibition rates of cell growth in ASC and VSC in co-culture or independent culture was detected after exposed to excitatory stimulus by MTT method. Real-time observation of ROS production by ASC and VSC labeled with Hoechst 33342 was detected by confocal microscopy under the conditions of co-culture or independent culture. RESULTS: Higher concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in ASC alone, while lower concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in VSC only. Real-time observation by confocal microscopy showed that ROS production by VSC under the condition of co-culture, which showed a notable increase at 15 min, was significant less than that in independent culture, which peaked at 5 min and was gradually decreased. ROS production by ASC in co-culture began to increase significantly at 10 min. CONCLUSION: Compared to independent culture, ASC reduces the resting ROS production by co-cultured with VSC, while ASC prolongs the duration of ROS production by VSC after exposed to excitatory stimulus.     

Effects of diabetes on the development of heart failure in streptozotocin-induced diabetic rat model with acute myocardial infarction

AIM: To evaluate the time course and effect of diabetes on the development of heart failure (HF) in poorly controlled streptozotocin (STZ) -induced diabetic rat model for 70 d with acute myocardial infarction (AMI) in vivo. METHODS: All SD rats were randomized into four groups. Diabetes were induced by a single intraperitoneal injection of STZ (65 mg/kg), and 70 d later after the induction, AMI models were made with the ligation of left anterior descending coronary artery. The time course of diabetic effects on the development of heart failure in rats before and after AMI was observed. The survival rate of the rats and ultrastructure change of myocardium, the hemodynamics, the extent of the myocardial fibrosis, and the cardiac hypertrophy were also determined. RESULTS: After the ligation of left anterior descending coronary artery, the diabetic rats showed worse LV function and accelerated left ventricular (LV) remodeling compared with the non-diabetic ones. Myocardial fibrosis in both diabetic and non-diabetic rats subjected to AMI was similar in the early phase, while it was quite different after 1 month. CONCLUSION: Heart failure progression is accelerated in diabetic rat with AMI.     

高校信息化建设和管理模式的探讨

本文针对高校信息化建设和管理中存在的问题以及笔者在高校信息化建设和管理中的亲身感受,在认真思考的基础上,提出了建设和管理统一化的设想.     

不同前处理对测定甜高粱茎秆汁液3种糖含量的影响

茎秆含糖量是甜高粱（Sorghum dochna）重要的农艺性状,其可溶性糖的组分与含量的测定,是甜高粱QTL定位和性状改良的前提。本研究以粒用高粱的‘忻粱52’与甜高粱‘W452’重组自交系F8代255个群体为材料,采用改良蒽酮比色法来测定蔗糖、果糖和葡萄糖含量。结果表明：可溶性总糖的最佳显色时间为10 min,显色温度为100℃;测定蔗糖的最佳处理条件为1 mol·L^-1的KOH溶液处理10 min;果糖测定的最佳显色温度为50℃,显色时间为3 min。优化过后的蒽酮比色法能测得较稳定的数值,精密度和稳定性较好,可用于测定甜高粱汁液的糖含量。重组自交系后代糖锤度与可溶性糖总量之间呈极显著正相关关系（P <0.01）,相关系数为0.885,线性回归方程为y=37.082×可溶性糖总量+3.990。     

甘南高寒牧区甘农2号小黑麦与箭筈豌豆的混播效果

通过研究甘农2号小黑麦(×Triticosecale W i t t m a c k'G a n n o n g N o.2')与3种箭筈豌豆(Vicia sativa)的最佳混播组分和混播比例,采取裂区设计,主区为混播组分,副区为混播比例.试验测定了3个箭筈豌豆(Vicia sativa)品种绿箭1号('Lvjian No.1')、绿箭2号('Lvjian No.2')、绿箭431('Lvjian 431')与甘农2号小黑麦在不同混播比例(0?100、20?80、30?70、40?60、50?50、60?40、70?30、80?20、100?0)下的生产性能和营养价值.结果表明,混播组分间,绿箭431和甘农2号小黑麦混播时的干草产量较高,营养价值较好,综合评价值最高(0.9416);混播比例间,50?50混播处理混播效果最佳.交互作用表明,绿箭431和甘农2号小黑麦以50?50的比例混播时干草产量最高(11.15 t·hm?2),粗蛋白含量为13.25％,营养适中,综合评价值最高(0.8321),混播效果最好.本研究为甘南高寒牧区禾–豆混播草的科学建植和管理,抗灾保畜、提升草地生产力和生态保障能力提供理论依据.     

石家庄市民心河水质预测分析

通过对石家庄市民心河沿线设立不同的监测点,选择对民心河景观水体影响较大的BOD5、氨氮、高锰酸钾等指标进行长期的监测,并根据2001~2006年水质监测数据,运用GM(1,1)模型建立了民心河水质预测模型,预测了民心河水质变化的趋势和规律,为民心河的治理和管理提供依据。     

麻核桃资源保护与开发利用研究

麻核桃(juglans hopeiensisHu)不仅是核桃属植物中分布范围最窄,数量最少的一个种,而且是我国特有的种,但是多年来没有引起足够重视,研究报道很少,本文对麻核桃资源和生产情况进行了初步调查,并根据坚果外观变化鉴定出11个类型,目的是为资源保护和良种选育奠定基础。     

扬州市古运河生态环境林观光休闲型绿化模式营建研究

结合对扬州市古运河的整治，营建生态环境林观光休闲型滨河绿地风光带。在其实施的第1期工程中，就观光休闲型绿化模式的定位功能、建模原理、模式结构、绿化设计原则、主要地段景点的绿化配置与造景造意，以及模式绿化的初期效果等进行初步研究探讨。要求通过增加乔木数量，配置观赏人工植物群落，搭配仿古园林建筑，保护古文化遗迹，形成滨河绿地森林公园，最终达到既发挥观光旅游、休闲娱乐游憩功能，又发挥净化空气、改善城市生活环境的生态功能的目的。     

祁连山区生态环境质量评价指标体系的构建

从自然环境、社会环境、环境退化及污染状况、生态环境治理状况、效益等方面对祁连山生态环境质量评价指标进行研究,提出了祁连山生态环境质量评价指标体系的构建思路及其指标体系,并对模糊综合评价方法进行评述,试图对区域经济开发和生态环境建设提供科学依据。     

观赏槭树的苗期试验初报

观赏槭树育苗试验表明,不同树种、种源间种实发芽率差异较大。发芽率较高的种类有樟叶槭(92%)、鸡爪槭(90%)、三角枫(90%),其次是复叶槭(85%)、红花槭(82%)、橄榄槭(76%)和元宝槭(75%);茶条槭、五角槭、罗浮槭发芽率均小于70%。1年生苗高生长量以复叶槭最大(113.98 cm),其次是三角枫(101.21 cm);樟叶槭和鸡爪槭1年生苗生长量中等,当年生长量分别为62.43 cm和71.74 cm;罗浮槭、青榨槭和橄榄槭1年生苗高生长量较小。季节生长节律有3种类型:橄榄槭、鸡爪槭、元宝槭、茶条槭等几种槭树表现为第1类型(前期生长型),即5月前苗木缓慢生长,6~7月进入速生期,7月以后苗木渐渐停止生长,速生期较短。复叶槭和青榨槭表现为第2种类型:1年生苗全年高生长有2次高峰,出现在6月和9月;樟叶槭和三角枫季节生长节律表现为第3种类型:5月生长缓慢,6月生长开始加快进入速生期,一直持续到8月底,速生期长。三角枫、橄榄槭、鸡爪槭、元宝槭、茶条槭、复叶槭和青榨槭等几种槭树表现出较强的抗寒性,樟叶槭和罗浮槭抗寒性较差。