Establishment of Cyperus rotundus Tubers Fingerprints by Magic Chemisorber-thermal Desorption-GC/MS Method

采用新型搅拌棒萃取器(MC)顶空萃取-热解吸-气相色谱/质谱(TD-GC/MS)方法分析香附中的挥发性成分,优化了MC萃取、解吸等条件,并对方法重现性进行考察,30个峰的相对峰面积的RSD均小于8.6％.继而测定了不同产地的香附样品,建立指纹谱图,标定44个共有峰,占总峰面积90％以上.并对图谱进行相似性评价及聚类分析,研究表明该法可为香附药材质量控制、产地选择提供理论依据.     

“威代尔”葡萄单株负载量对浆果品质和枝蔓贮藏营养的影响

通过单株保留8、12、16、20和24个果穗数的试验处理,研究了不同产量指标对冰酒葡萄品种“威代尔”果实品质和枝蔓可溶性糖、淀粉和木质素含量的影响,以期为合理确定产量指标提供依据.结果表明:单株留穗数在16～20个、单株负载量3.5～4.4kg、单位面积产量1 330～1 680 kg/667m2,有利于提高葡萄浆果品质和枝蔓可溶性糖、淀粉和木质素的含量.     

应用性诱剂防治甘蔗螟虫

对甘蔗螟虫的为害特点和防治现状以及性诱剂的特点和应用现状进行了概述。为了有效防治甘蔗螟虫,利用性诱剂进行预测预报,指导适时防治具有重要意义。     

桂林地区苗用型大白菜新品种引种品比试验

对引进的3个苗用型大白菜品种进行品种比较试验。试验结果表明,浙白6号综合性状最好,可作为夏季栽培的首推品种,德高快菜次之,双耐增产率虽稍低,但其植株生长健壮,叶片较厚且有光泽,耐热性好,抗病性强。引进的3个新品种均适合在桂林地区夏秋作为绿叶蔬菜栽培。     

Dynamic expression of thioredoxin 2 in myocardial mitochondria in selenium-deficiency rats during myocardial injury

AIM: To investigate the dynamic expression of thioredoxin 2 (Trx2) protein in the myocardial mitochondria under the condition of selenium deficiency. METHODS: A model of selenium deficiency was made using two-week-old SD rats. When the rats were fed for 20 weeks, 30 weeks and 40 weeks, the cardiac functions were detected by carotid artery intubation. The myocardial mitochondria were also extracted, and the protein expression of mitochondrial Trx2 was measured by Western blotting. The method of immunohistochemistry was used to detect Fas-associated death domain protein (FADD,a death marker protein) for determining the apoptosis of myocardial cells. The correlation between Trx2 and left ventricular systolic pressure (LVSP), maximum rate of left ventricular pressure rise(+dp/dtmax) and apoptotic index of the myocardial cells was analyzed. RESULTS: Compared with the corresponding period of normal control group, the expression of Trx2 protein in the selenium-deficiency rats was reduced. The expression of Trx2 protein was continuously reduced as the time of selenium deficiency prolonged. As the time of low-selenium feeding was going on, the systolic and diastolic functions of the heart were impaired, and the number of apoptotic myocardial cells was increased. The correlation analysis indicated that Trx2 had positive correlations with LVSP and +dp/dtmax, and had a negative correlation with the apoptotic index of myocardial cells. CONCLUSION: Selenium deficiency affects the expression of Trx2 protein,and causes impaired cardiac functions and the apoptosis of myocardial cells. Trx2 has a protective effect on myocardial cells.     

极具潜力的重组药用蛋白的毛状根表达系统研究

对植物表达系统产生重组药用蛋白进行了简单的介绍,重点论述了利用发根农杆菌诱导的毛状根培养系统表达重组药用蛋白的研究及应用情况。     

非灭菌土接种AM真菌对油蒿抗旱性的影响

利用盆栽试验在正常水分和干旱胁迫条件下研究了非灭菌土接种AM真菌摩西球囊霉(Glomus mosseae)和土著AM真菌(Indigenous arbuscular mycorrhizal fungi)对油蒿(Artemisia ordosica)生长及抗旱性的影响。结果表明:干旱胁迫显著抑制了AM真菌对油蒿的侵染。无论在正常水分还是干旱胁迫条件下,接种AM真菌都增加了植株的分枝数、地上部鲜重和干重、地下部鲜重和干重,但没有明显提高株高、茎粗和改善组织水分状况;与未接种相比,干旱胁迫下接种土著AM真菌显著提高了根系氮、磷含量,提高了叶绿素、可溶性蛋白含量,增强了保护酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性并降低了丙二醛的含量,因此增强了植物的抗旱性。     

不同覆盖物对果树越冬的保温作用

在克拉玛依市乌尔禾区,于2006～2007年冬季,采用不同覆盖材料,进行了黑塑料布、双膜彩条布、单膜彩条布和紧贴式双层塑料布覆盖下的最低温度和温差的逐日观测,以减轻果树越冬掩埋的劳动强度。结果表明:最低气温平均值是黑塑料布-5.9℃,双膜彩条布-7.3℃,单膜彩条布-9.3℃,紧贴式双层-6.6℃,裸露的气温-17.9℃。温差平均值是黑塑料布-2.8℃,双膜彩条布-2.6℃,单膜彩条布-4.8℃,紧贴式双层-3.7℃,裸露的气温-10.0℃。试验表明,黑塑料布的保温效果好,温差也小。单膜彩条布的保温效果最差,温差也最大,有转暖乍冷的不良影响。紧贴式双层塑料布的保温效果因为与单层黑塑料布相同,并且多浪费一层覆盖材料和多费工时而被否定。     

不同培养基在洋葱组培中的应用

为完善洋葱组培技术,针对不同的洋葱品种,使用不同的基础培养基,添加不同的生长调节剂进行培养,结果发现：洋葱品种早春黄在培养基MS+6 mg•L^-1 6-BA+0.1 mg•L^-1 NAA上增殖效果最好,连葱6号在培养基3 g•L^-1花宝+6 mg•L^-1 6-BA+0.1 mg•L^-1 NAA上增殖效果最好;在继代培养过程中添加0.25 mg•L^-1多效唑可以提高增殖率。     

‘川花梨’品种特性及优质高产栽培技术

以‘川花梨’为试材,通过调查研究其品种特性,探讨优质高产栽培技术。结果表明:利用配方施肥技术能针对性地补充土壤和植株养分,防止滥用化学肥料;果实二次套袋技术则使果面更加光洁,提高其商品性;采取疏散分层的树形便于接受阳光,丰产性更好;加强疏花疏果使叶果比保持(40～45):1,控制植株负载量,可溶性固形物可提高2%左右;生育期采用黄色粘虫板、糖醋液杀虫瓶和频振式杀虫灯显著减少了生长季节农药施用量20%;采果后施肥保叶防止开秋花;做好秋冬季管理大大减少了病虫基数和源头,增强了树势,提高抗病虫害能力。