Efficacy of imiquimod 5% cream in the treatment of equine sarcoids: a pilot study

Imiquimod is an immune response modifier with potent antiviral and antitumour activity. The objective of this pilot study was to evaluate the efficacy of an imiquimod 5% cream (Aldaratrade mark: 3M, Saint Paul, MN, USA) as a topical treatment for equine sarcoids. Fifteen horses with a total of 19 tumours were enrolled, including mixed (7), fibroblastic (5), flat (3), verrucous (2), and nodular (2) types. Baseline data included history, physical examination, tumour location, measurement and digital photography. Imiquimod was applied by the owners three times a week until complete resolution of the tumour or 32 weeks, whichever occurred first. Tumours were measured and photographed every 4 weeks. Treatment efficacy was defined as 75% or greater reduction of tumour size by the end of the trial. Four sarcoids were withdrawn from the study. Twelve of the remaining 15 tumours (80%) showed more than 75% reduction in size and nine (60%) totally resolved between 8 and 32 weeks. The most common adverse effects of exudation, erythema, erosions, depigmentation and alopecia were limited to the tumour and adjacent areas. The results suggest that topical imiquimod is a therapeutic option for the treatment of equine sarcoids, although more detailed studies are required to corroborate these initial findings.     

Identification of a target‐site mutation conferring resistance to triazine herbicides in oriental mustard (Sisymbrium orientale L.) from Australia

In southern Australia, oriental mustard (Sisymbrium orientale) has been controlled successfully by triazine herbicides for several decades. The screening of 40 populations that were collected from the southern grain belt of Australia during 2010 and 2013 for resistance to six different herbicides (glyphosate, diflufenican, imazamox, chlorsulfuron, atrazine and 2,4‐dichlorophenoxyacetic acid) identified two oriental mustard populations as highly resistant to atrazine. Compared to the known oriental mustard‐susceptible populations (S1 and S2), these two resistant populations (P17 and P18) from near Horsham, Victoria, Australia, were 311‐ and 315‐fold resistant to atrazine, as determined by a comparison of the LD₅₀ values. However, there was no resistance to diuron detected in these populations. Sequencing of the chloroplast psbA gene identified a missense mutation of serine 264 to glycine in both herbicide‐resistant oriental mustard populations, which is known to confer high‐level atrazine resistance in other species.     

The efficacy of imiquimod 5% cream (Aldara®) in the treatment of aural plaque in horses: a pilot open‐label clinical trial

Aural plaques affect at least 22% of horses and can be asymptomatic or cause ear sensitivity. Immunohistochemical and electron microscopy studies have shown a strong association between aural plaques and papilloma virus. The purpose of this study was to investigate the efficacy of imiquimod 5% cream, an immune response modifier with potent antiviral activity, in the treatment of equine aural plaques. Twenty‐one horses were enrolled and 16 completed the study. Imiquimod 5% cream was applied three times a week, every other week. When both ears were affected only the worst affected ear was treated. Adverse effects in all horses included marked local inflammation, exudation and thick crust formation at the site of treatment and the adjacent skin. Removal of the crust before treatment was painful and required sedation in most horses. Complete resolution of lesions was noted in all horses immediately post‐treatment and the long‐term resolution rate was 87.5%. Duration of therapy ranged from 1.5 to 8 months (median: 2.9 mean: 3.5). All horses were followed‐up for 12–22 months after treatment was discontinued and only two horses had a recurrence of lesions. Clinical signs related to the aural plaques prior to treatment were reported in 11 of 16 (68.8%) horses and included resistance to touching the ears and bridling. Complete resolution of these signs was reported by the owners in all of the horses followed‐up for at least 12 months. In conclusion, the topical application of imiquimod 5% cream is an efficacious treatment for aural plaques in horses.     

A phase II study to evaluate the toxicity and efficacy of alternating CCNU and high‐dose vinblastine and prednisone (CVP) for treatment of dogs with high‐grade,metastatic or nonresectable mast cell tumours

Safety and efficacy of a protocol of alternating 1‐(2‐chloroethyl)‐3‐cyclohexyl‐1‐nitrosourea (CCNU; 70 mg m^?2) and vinblastine (3.5 mg m^?2), and prednisone (1–2 mg kg^?1; CVP) in dogs with mast cell tumours (MCT) were evaluated. A total of 17 dogs had nonresectable MCTs and 35 received CVP as adjunctive treatment to locoregional control of metastatic MCTs or grade III MCTs. Neutropenia with fever occurred in 8% of dogs after treatment with vinblastine and in 2% after treatment with CCNU. Persistent elevation of serum alanine transaminase, suggestive of hepatotoxicity, occurred in 9% of the dogs. Response rate in dogs with nonresectable MCTs was 65%; five achieved a complete response (median, 141 days) and six achieved a partial response (median, 66 days). Overall median progression‐free survival (PFS) time in dogs treated in the adjuvant setting was 489 days. Dogs with grade III MCTs had shorter PFS compared with dogs with metastatic grade II MCTs (190 days versus 954 days; P < 0.001). Phase III studies are needed to provide reliable information about the comparative efficacy of this protocol.     

Irrigation strategies to improve the water use efficiency of wheat-maize double cropping systems in North China Plain

Water is the most important limiting factor of wheat (Triticum aestivum L.) and maize (Zea mays L.) double cropping systems in the North China Plain (NCP). A two-year experiment with four irrigation levels based on crop growth stages was used to calibrate and validate RZWQM2, a hybrid model that combines the Root Zone Water Quality Model (RZWQM) and DSSAT4.0. The calibrated model was then used to investigate various irrigation strategies for high yield and water use efficiency (WUE) using weather data from 1961 to 1999. The model simulated soil moisture, crop yield, above-ground biomass and WUE in responses to irrigation schedules well, with root mean square errors (RMSEs) of 0.029 cm³ cm⁻³, 0.59 Mg ha⁻¹, 2.05 Mg ha⁻¹, and 0.19 kg m⁻³, respectively, for wheat; and 0.027 cm³ cm⁻³, 0.71 Mg ha⁻¹, 1.51 Mg ha⁻¹ and 0.35 kg m⁻³, respectively, for maize. WUE increased with the amount of irrigation applied during the dry growing season of 2001-2002, but was less sensitive to irrigation during the wet season of 2002-2003. Long-term simulation using weather data from 1961 to 1999 showed that initial soil water at planting was adequate (at 82% of crop available water) for wheat establishment due to the high rainfall during the previous maize season. Preseason irrigation for wheat commonly practiced by local farmers should be postponed to the most sensitive growth stage (stem extension) for higher yield and WUE in the area. Preseason irrigation for maize is needed in 40% of the years. With limited irrigation available (100, 150, 200, or 250 mm per year), 80% of the water allocated to the critical wheat growth stages and 20% applied at maize planting achieved the highest WUE and the least water drainage overall for the two crops.     

BOOK REVIEWS

Book reviewed in this article:
Guide to the Dissection of Domestic Ruminants . RE Habel
Biology and Medicine of Rabbits and Rodents . JE Harkness and JF Wagner     

Management of gastrointestinal pain.

Choice of an analgesic for gastrointestinal pain requires consideration of the cause of the pain, desired duration of pain relief, need for sedation, and potential side effects and toxicity, particularly in light of other drugs being used and effects on the gastrointestinal tract. It is imperative that close monitoring be continued to ensure that surgical lesions or worsening conditions are detected. Recent research in the field may lead to new drugs, drug combinations, and avenues of treatment that minimize the side effects of these drugs while maximizing their efficacy.     

INFLUENCE OF INHALED SMOKE FROM UPPER RESPIRATORY LASER SURGERY

Endoscopic tracheal lavage cytology was evaluated before and 24 hours after standing transendoscopic laser surgery of the upper airway in 18 horses (9 patients undergoing Neodymium:yttrium aluminum garnet [Nd:YAG] surgery and 9 non-patient horses undergoing argon surgery). Paired endoscopic tracheal lavages taken 24 hours apart were evaluated in six control horses to determine if the lavage technique influenced the cytology. After 24 hours, no inflammation was noted in three, mild inflammation was noted in six and suppurative inflammation in four surgically treated horses. Five of the surgically treated horses underwent general anesthesia prior to baseline tracheal lavage and had evidence of inflammation in pre-laser lavage samples; this made it difficult to assess the true incidence of laser-induced inflammation in horses in this report. Five of six control horses had a normal tracheal lavage at 24 hours. Six normal adult horses had baseline tracheal mucous clearance rates determined using scintigraphy followed one week later by tracheal mucous clearance rates two hours after either standing transendoscopic Nd:YAG ventriculectomy or standing transendoscopic argon cauterization of a 2 × 2 cm area of dorsal pharynx. The horses were left in pasture for three weeks, followed by a second baseline and post-laser tracheal mucous clearance rate using the opposite laser technique. There was no significant difference between the first and second baseline measurements. The order of laser treatments had no effect on the results obtained. There was no significant difference between the baseline tracheal mucous clearance rates (mean 1.65 ± 0.65 cm/min) and tracheal mucous clearance rates post-laser surgery (mean 1.62 ± 1.21 cm/min). Neither laser technique altered tracheal mucous clearance rates significantly (argon mean 1.57 ± 0.59 cm/min and Nd:YAG mean 1.67 ± .68 cm/min.