6-BA在葡萄植株体内的运转和分配

以葡萄为试材,研究了葡萄根、茎、叶、果等器官对6-苄基腺嘌呤(6-BA)的吸收及它在植株内的运转和分配。结果表明,对1年生葡萄幼苗茎部引入或叶片涂抹6-BA,1h后,在植株所有部位都有分布,其中以茎尖和根部浓度较高,表现为快速、非极性的运输特性。茎部引入5d之内,植株根内6-BA呈持续升高趋势,茎尖内6-BA虽然有波动,但是最后仍达到最大;而叶片涂抹则在涂抹后5h时,全株各部位6-BA浓度达到最大,5d后,除引入叶片外,其它部位几乎测不到6-BA。盛花后30d,果穗浸蘸和穗轴引入6-BA,2h后,果皮、果肉、种子中6-BA浓度达到高峰,以后逐渐下降,果穗浸蘸30d后,果实各部位均已测不到6-BA,穗轴引入30d后,果皮和果肉内仍能测到6-BA,但种子内已测不到。     

桃流胶病研究进展

流胶病为桃树生产上的主要病害之一,发生普遍,危害严重。综述了引起桃流胶病的致病菌种类,病原菌的发生与侵染规律,组织病理学研究,田间自然条件与人工接种的抗性鉴定及筛选出的抗性品种,所采取的综合防治措施,以及在抗性育种与抗病性筛选的生理生化指标方面的研究进展,并对今后的工作重点提出了建议。     

Expression of IL-6 mRNA in endometrium with endometriosis

AIM: The purpose of the present study was to explore the relationship between interleukin-6 mRNA expression and endometriosis. METHODS: Using the rat model, IL-6 mRNA expression in the endometrium was examined by RT-PCR. RESULTS: The expression of IL-6 mRNA in control rats did not change at 2, 4, 6 and 8 weeks after sham operation (P>0.05), but in model rats it gradually increased at 2, 4, 6 and 8 weeks after endometriosis (P<0.01). The expression of IL-6 mRNA in uterine endometrium with endometriosis was lower than in endometriotic tissue, but higher than in endometrium from healthy controls. CONCLUSION: The IL-6 mRNA expression may contribute to the development of endometriosis . The increase in IL-6 mRNA expression may promote the implantation and growth of endometriotic tissue.     

The genetic susceptibility of HLA-DRB1 alleles in esophageal neoplasm of Hubei Han Chinese

AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal neoplasm in Hubei Han Chinese. METHODS: HLA-DRB1 gene polymorphism in 42 patients with esophageal neoplasm and 136 normal control subjects was studied by PCR and sequence. RESULTS: Allele frequency of HLA-DRB1 *0901 allele was significantly higher in esophageal cancer patients than those in normal controls(0.2500 vs 0.1397, P =0.028; the odds ratiO².053; etiologic fraction 0.1282).There were no association between the rested HLA-DRB1 alleles with patients. CONCLUSION: Individuals carrying HLA-DRB1 *0901 may be susceptible to esophagealo carcinoma, its nucleotide sepuence approachs to the corresponded allele sequence(exoN²)published in GenBank.     

低温胁迫下弓葵幼苗膜脂过氧化及保护酶活性的变化

 低温胁迫下弓葵( Butia capitata Becc) 幼苗叶片的MDA 含量逐渐增加, 膜脂过氧化作用增强。- 8 ℃条件下的膜脂过氧化作用明显强于2 ℃。细胞膜透性在2 ℃条件下变化不大, - 8 ℃时则随低温胁迫时间延长而急剧上升, 细胞膜受到伤害。- 8 ℃胁迫下细胞保护酶SOD、POD 和CAT 活性短期(6 h) 内升高,然后下降, 24 h 以后3 种保护酶受到低温胁迫的严重抑制。在2 ℃胁迫下, SOD 活性在6 h 内变化不大, 随后下降; CAT活性变化趋势与- 8 ℃时相似, 但变化幅度较小; POD 虽也呈现先升后降的趋势, 但降幅明显小于升幅, 至48 h 时POD 活性仍维持较高水平。2 ℃低温胁迫不是抑制而是促进POD 活性的提高。     

植酸酶在饲料中的研究与应用

植酸酶在饲料中应用，不仅能提高植酸磷的消化率，减少配方中无机磷的添加量和磷排泄污染，还可改善和提高蛋白质、能量、氨基酸和微量元素的利用率。     

加强自身卫生防护防止传播动物疫病

随着我国畜牧养殖业的不断发展，动物防疫检疫人员在依法严格把关、确保动物及其产品检疫质量、保护人民身体健康、促进养殖业健康发展等方面，做出了积极努力和贡献。但动物防疫检疫人员作为与各种动物及其产品的密切接触者，其自身对消毒防护措施的重视程度，与动物疫病的发生和流行有着一定的关系。     

南瓜新品种龙园栗香的选育

龙园栗香南瓜是从日本和韩国引进品种中 ,经分离选育的自交系 93- 12 - 8和 93- 9- 7配制的早熟南瓜一代杂种 ,果实扁圆形 ,果皮墨绿色 ,覆白绿色点条斑纹 ,平均单瓜质量 2 .0kg ,果肉桔黄色 ,可食率达 80% ,肉质香面适口、品质佳。从播种至采收 80d(天 )左右 ,较抗白粉病和病毒病 ,平均产量逾 5 0 0 0 0kg·hm-2 。     

Rat mesenchymal stem cells differentiate into neuron-like cells induced by berberine in vitro

AIM: To investigate whether berberine can induce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro. METHODS: MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to differentiate by berberine. The morphological changes of MSCs were evaluated by light microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. RESULTS: Induced by berberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: Berberine may induce adult rat MSCs to differentiate into neuron-like cells in vitro.     

Relation between TNF-α,TNFR I expression and apoptosis in oral lichen planus

AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus.