Investigations of the maintenance system of the Konik Polski horse and its effects on fecal microbiota activity during the winter and summer seasons

Gastrointestinal microbiota play a key role in the nutrients digestion and hence maintaining animal health and welfare. The diet offered to the animals in captivity may differ considerably from that on natural pastures. In a stabled maintenance system, horses have a limited choice of habitat and feed. Time spend for feeding is relevant for equine welfare because the reduction of the time devoted for foraging may be responsible for inducing gastric inflammation and ulceration. Therefore, in the present study, it was hypothesized that fecal bacterial fermentative processes differ between free-roaming and stabled Konik Polski Horses (KPHs) with respect to microbial enzymatic activity, and thus the fecal concentration of short-chain fatty acids (SCFA), which can be further utilized for assessing the feeding behavior and welfare in free-roaming versus stabled horses. The SCFA concentration and profile, as well as the extracellular and intracellular activities of selected bacterial enzymes, were characterized in horse feces collected during the winter and summer feeding seasons. The results showed higher enzymatic activity and SCFA production in the feces excreted by free-roaming versus stabled horses, especially during summer. An increase in pasture plant diversity may be beneficial for the gastrointestinal microbiota and hence for maintaining health and welfare.     

猪丹毒

猪丹毒又称“红热病” “打火印”或“钻石皮肤病”,是一种急性的、热性的传染病,引起该病的病原为红斑丹毒丝菌,可以通过伤口感染人,也是一种人兽共患病。患病猪的临床表现形式大致可分为3种类型,即急性、亚急性和慢性型。急性型病例主要的表现为出现明显的败血症症状。亚急性型病例则是在四肢、胸腹部、背部等皮肤处出现红色菱形、方形或圆形疹块。慢性型则多以出现关节炎和心内膜炎引起的关节肿胀、运动障碍和心跳加速、呼吸急促为主的临床表现;有时还可见皮肤坏死。红斑丹毒丝菌的最易感动物是猪,没有品种和年龄差异。使用疫苗是最佳的预防手段,治疗方面除使用抗生素外还可以选择中药方剂进行治疗。     

Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.     

Two-level Fuzzy Method for Evaluation of the Supplier''s System in Automobile Industry

This paper analyses the importance of the suppliers' evaluation in automobile industry. Based the conception of system, all the corporations' suppliers are regarded as an integer. On condition that the corporation has evaluated the single supplier's performance, the model for evaluate the performance of supplier's system is established, the performance by two-level fuzzy method is evaluated. Finally, the method sets with an example as the illustration.     

Pd Pattern Recognition Based on Linear Discriminant Analysis in GIS

According to the character of PD in GIS, the authors design four kinds of GIS defection models. The GIS gray intensity images are constructed based on mass specimens gathered by the ultra - high frequency and high speeds systems, Aiming at the PD characteristics and its defections, A PCA-FDA method is put forward based on PD images. The principal component analysis is employed to condense the dimension of PD images, then the optimal sets of statistically uncorrelated discriminant vectors are extracted, and the minimum distance classifier is constructed as classifier. The identified results show that this method can effectively elevated the discrimination of the four kinds of defects in GIS PD.     

Simulation Propagation of Partial Discharge UHF Signals in Transformer

Many factors affect the propagation of ultra high frequency(UHF) signals caused by partial discharge(PD) on the waveforms and energy of signals in transformer.To investigate the relationshi Pof factors and UHF signals,simulation model of transformer for PD study are established.UHF signals in simulation model of transformer caused by PD are calculated using finite difference time domain method.By means of amplitude and cumulative energy plot of simulation signals,and combing the measured result,the following factors:distance between PD source and detection position,different metallic conductor position around detection position and iron core and winding are researched,which contribute to comprehend the propagation characteristic of UHF signals and detect UHF signals accurately.     

Detecting System of the Dielectric Loss in Capacitive Equipment Based on Virtual Instruments

A digital detecting system of the dielectric loss in the capacitive equipment is introduced based on virtual instruments.This system can real-time collect,analyze,process and storage electrical signals with National Instruments' program LabVIEW and ADLINK,s DAQ board.Through the simulation test in lab and the real test in electric substation,it is shown that this system has high accuracy on testing the dielectric loss in the capacitive equipment,the real test data is stable and the relative error of real dielectric loss is less than 5%.It is to say that this system can meet the requirement of the real on-line monitoring.     

离心式泥浆泵水力设计探讨

介绍了一种适用于水利工程全方施工的立式泥浆泵，并根据该泵的特点，对其水力设计方法作了初步探讨。     

江苏省水稻节水灌溉技术推广模式

针对江苏省稻作区水资源、土壤、气候的特点 ,选择浅湿灌溉、浅湿调控灌溉、控制灌溉、水稻旱作灌溉等 4种技术因地制宜进行推广。在灌溉过程中 ,建立和完善了一整套的组织措施和技术措施 ,形成了江苏省水稻节水灌溉技术推广模式 ,取得了显著的效果 ,丰富了水稻节水灌溉技术推广的内容     

基于距离反比法的土壤盐分三维空间插值研究

以新疆兵团一块面积约70 hm2的盐碱地为研究对象,采用EM38与土钻取样相结合的方法得到了126个点不同土层(0~200 cm)的1 386个土壤盐分数据,应用三维距离反比法(3D-IDW)对土壤盐分进行了空间插值,探讨了垂向坐标扩大倍数和搜索点数对插值结果精度的影响。结果表明,研究区0~140 cm土层盐分平均含量较高,为1.84~2.11 g/kg,盐分变异较大,而140~200 cm土层盐分平均含量较低,为1.74~1.79 g/kg。所有土层盐分含量的统计特征值(平均值、标准差和变异系数等)均随土层加深而呈现递减的趋势。土壤盐分实测值和估计值的均方根误差随垂向坐标扩大倍数的增大而减小,随搜索点数的增加而增大,其值在0.1~0.4 g/kg范围内变化,当垂向坐标扩大300倍、搜索点数为6个时,插值效果较优。采用确定的参数对研究区的土壤盐分进行了三维空间插值,结果表明土壤盐分空间分布特征与实测值比较吻合,大部分区域土壤盐分含量小于2.5 g/kg,靠近北部和南部边界区土壤含盐量较低,属于非盐化土区,而大于4 g/kg盐化土主要分布在中间和南部局部区域。研究区80%土壤为非盐化土和轻度盐化土,20%为中度和重度盐化土。影响该区盐化土分布的主要因素有灌溉、局部地形、粘土层位置、地下水埋深和矿化度。当不同方向的取样间距相差很大时,选取合适的垂向坐标扩大倍数和搜索点数对保障3D-IDW法的插值结果精度至关重要。