Pigmentation and spectral absorbance signatures in deep-water corals from the Trondheimsfjord, Norway

The pigmentation and corresponding in vivo and in vitro absorption characteristics in three different deep-water coral species: white and orange Lophelia pertusa, Paragorgia arborea and Primnoa resedaeformis, collected from the Trondheimsfjord are described. Pigments were isolated and characterized by High-Performance Liquid Chromatography (HPLC) analysis and High-Performance Liquid Chromatography Time-Of-Flight Mass Spectrometer (LC-TOF MS). The main carotenoids identified for all three coral species were astaxanthin and a canthaxanthin-like carotenoid. Soft tissue and skeleton of orange L. pertusa contained 2 times more astaxanthin g(-1) wet weight compared to white L. pertusa. White and orange L. pertusa were characterized with in vivo absorbance peaks at 409 and 473 nm, respectively. In vivo absorbance maxima for P. arborea and P. resedaeformis was typically at 475 nm. The shapes of the absorbance spectra (400-700 nm) were species-specific, indicated by in vivo, in vitro and the corresponding difference spectra. The results may provide important chemotaxonomic information for pigment when bonded to their proteins in vivo, bio-prospecting, and for in situ identification, mapping and monitoring of corals.     

Status and the potential for extinction of the largetooth sawfish Pristis pristis in the Atlantic Ocean

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Reproductive performance in sows in relation to Japanese Encephalitis Virus seropositivity in an endemic area

Japanese Encephalitis Virus (JEV) is considered an important reproductive pathogen in pigs. Most studies of the reproductive impact of JEV have been conducted in areas where the disease occurs in seasonal epidemics. In this study, the associations between seropositivity for JEV, measured with an IgG ELISA, and the number of piglets born alive and stillborn were investigated in a tropical area endemic for JEV in Vietnam. Sixty percent of sows from four farms in the Mekong delta of Vietnam were seropositive to JEV and the Odds Ratio for a sow being infected was highest (6.4) in sows above 3.5 years (95% confidence interval 2.2–18.3). There was an association between increasing Optical Density (OD) values from the ELISA and the number of stillborn piglets in sows less than 1.5 years, but no effect of seropositivity could be shown when all sows were studied. OD values had an effect (p = 0.04) on the number of piglets born alive in the statistical analysis only when interacting with the effect of the breeds. An increase in mean OD value of the herd was correlated (p < 0.0001) with an increase in the number of piglets born alive. In this study, there was evidence of a negative association between seropositivity for JEV and the reproductive performance only in sows less than 1.5 years in endemic areas. This could be explained by a year-round infection with the virus, which would lead to immunity in many gilts before their first pregnancy. This, in turn, may imply that JEV infection in pigs is of minor importance for the reproductive performance in endemic areas.     

Patterns of field layer invertebrates in successional stages of managed boreal forest: Implications for the declining Capercaillie Tetrao urogallus L. population

Capercaillie (Tetrao urogallus L.) populations in Finland have decreased markedly during past decades. One of the assumed reasons is the decreased quality of brood feeding grounds since current forest management transfers mature forests to younger successional stages. We studied how different types of managed Finnish forests offer resources for capercaillie broods by comparing the vegetation and invertebrate fauna of four successional stages in the two most common forest types in Finland, spruce dominated Vaccinium myrtillus (MT) and pine dominated Vaccinium vitis-idaea (VT) type. Forest age class had a significant effect on the cover of bilberry. There was a positive correlation between bilberry cover and the biomass of larvae and of all invertebrates (including all developmental stages) both at the stand and the sample level. Both forest type and age class significantly affected the biomass of larvae. Mature stands and young stands had the highest biomass of larvae in both MT and VT. In both forest site types the sapling stands hosted the smallest biomass of larvae. The results suggest that successional stages that follow clear cutting seriously lowers the food availability for capercaillie chicks and also reduces the shelter that field layer might provide. However, already the young stages that follow sapling phases seem to provide both bilberries and invertebrates so it is quite unlikely that the bilberry abundance alone could explain the dramatic decline of the capercaillie.     

Detecting potato viruses using direct reverse transcription quantitative PCR (DiRT-qPCR) without RNA purification: an alternative to DAS-ELISA

Rubber wood, one of the most preferred plantation timbers in the tropical regions, is easily prone to various moulds, decay and stain fungi. Lasiodiplodia theobromae, the dominant sapstain fungus of rubber wood, causes high economic loss to the industry through its bluish black sap wood discolouration. In our previous laboratory trials, Bacillus subtilis B1 isolated from market available compost was proved as a potent biocontrol agent against the sapstain fungus L. theobromae. The main aim of the present study was to demonstrate the biocontrol potential of B. subtilis B1 against rubber wood sapstain fungus on fresh rubber wood pieces during pre-monsoon, southwest monsoon and northeast monsoon seasons in the field. The visual ratings provided for the wood fungal growth and internal wood stain exhibited the bio-protectant efficiency of B. subtilis B1 even in the southwest monsoon season, which is considered as the most favourable period for the wood fungal growth. Biocontrol agent, B. subtilis B1 demonstrated significant inhibitions of sapstain fungus on open stacked rubber wood pieces in all the seasons. The frequent rainfalls during monsoon periods provide a conducive environment for fungal growth on felled rubber wood. Successful field evaluations of the biocontrol agent during pre-monsoon, monsoon and post-monsoon periods would guarantee year round protection against rubber wood sapstain. B. subtilis B1 displayed holistic biocontrol efficiency towards other stain (Ceratocystis sp., Fusarium sp.), mould (Aspergillus sp., Penicllium sp.) and decay fungi (Trametes versicolor) as well.     

Biopore history determines the microbial community composition in subsoil hotspots

Biopores are hotspots of nutrient mobilisation and shortcuts for carbon (C) into subsoils. C processing relies on microbial community composition, which remains unexplored in subsoil biopores. Phospholipid fatty acids (PLFAs; markers for living microbial groups) and amino sugars (microbial necromass markers) were extracted from two subsoil depths (45–75 cm ; 75–105 cm) and three biopore types: (I) drilosphere of Lumbricus terrestris L., (II) 2-year-old root biopores and (III) 1.5-year-old root biopores plus six 6 months of L. terrestris activities. Biopore C contents were at least 2.5 times higher than in bulk soil, causing 26–35 times higher Σ PLFAs g^-1 soil. The highest Σ PLFAs were found in both earthworm biopore types; thus, the highest soil organic matter and nutrient turnover were assumed. Σ PLFAs was 33% lower in root pores than in earthworm pores. The treatment affected the microbial community composition more strongly than soil depth, hinting to similar C quality in biopores: Gram-positives including actinobacteria were more abundant in root pores than in earthworm pores, probably due to lower C bioavailability in the former. Both earthworm pore types featured fresh litter input, promoting growth of Gram-negatives and fungi. Earthworms in root pores shifted the composition of the microbial community heavily and turned root pores into earthworm pores within 6 months. Only recent communities were affected and they reflect a strong heterogeneity of microbial activity and functions in subsoil hotspots, whereas biopore-specific necromass accumulation from different microbial groups was absent.     

Suspected systemic calcinosis and calciphylaxis in 5 horses

Five horses were presented with signs of myopathy along with systemic malaise, hyperfibrinogenemia, hyperphosphatemia, and an elevated calcium phosphorus product (Ca*P). Postmortem findings were consistent with systemic calcinosis, a syndrome of calcium deposition in the tissue of organs including lungs, kidneys, muscle, and heart that has not been previously described in horses.     

Effects of ketamine-xylazine intravenous bolus injection on cardiovascular function in rabbits

The direct effects of ketamine-xylazine (KET-XYL) on vascular function have not been investigated in rabbits. The short-term cardiovascular effects of intravenous (IV) KET-XYL bolus injection, therefore, should be investigated using vascular ultrasonography.In this prospective experimental study, KET-XYL anesthesia was induced IV in 9 female New Zealand White rabbits before 3 defined test bolus injections of KET-XYL were given IV. Before and for 10 min after each KET-XYL injection vascular and hemodynamic variables were recorded at the left common carotid artery (ACC) after the 1st injection, and at the abdominal aorta (AA) after the 2nd injection. Echocardiography was performed after the 3rd injection to investigate changes in cardiac parameters.Ketamine-xylazine IV caused a significant increase in vessel diameter at the ACC and AA. Average volumetric flow significantly decreased at the ACC and pulsatility index significantly decreased at the AA. Fractional shortening (FS) and heart rate significantly decreased, while mean arterial blood pressure initially increased.Bolus injections of KET-XYL IV produced a transient vasodilatation at the ACC and AA. Despite central vasodilatation, bradycardia, and decrease of FS and average volumetric flow (VFave), mean arterial blood pressure did not significantly decrease indicating well-preserved cardiovascular compensatory mechanism after the ratio and doses of KET-XYL IV bolus injections used in this study.