植酸酶在饲料中的研究与应用

植酸酶在饲料中应用，不仅能提高植酸磷的消化率，减少配方中无机磷的添加量和磷排泄污染，还可改善和提高蛋白质、能量、氨基酸和微量元素的利用率。     

荔枝冷害过程中果皮色泽、花色素苷和类黄酮含量的变化

 研究了‘糯米糍’荔枝果实在3 ℃和0 ℃条件下果皮的色泽和花色素苷及类黄酮含量的变化。结果表明, 果实在0 ℃下14 d 时褐变指数迅速增大, 21 d 时已遭受不可逆的冷害。在冷藏过程中, 果皮的色泽指标a、L、C 值及花色素苷、类黄酮、总酚含量均随贮藏时间的延长而下降, 0 ℃下比3 ℃下降快。0 ℃下14～21 d , a、L、C 值分别下降了27.95 %、33.58 %和58.47 % , 而21 d 时果皮花色素苷、类黄酮和总酚含量依次为采收时的18.08 %、35.99 %和41.64 %。说明冷害加速了荔枝果皮花色素苷的降解及类黄酮和总酚的变化, 促进了褐变进程的发生。果皮a、L、C 值与褐变指数、花色素苷含量呈显著相关性( P < 0.05) ,采用自动测色色度计可真实地反映果皮花色素苷含量和褐变程度。     

Rat mesenchymal stem cells differentiate into neuron-like cells induced by berberine in vitro

AIM: To investigate whether berberine can induce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro. METHODS: MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to differentiate by berberine. The morphological changes of MSCs were evaluated by light microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. RESULTS: Induced by berberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: Berberine may induce adult rat MSCs to differentiate into neuron-like cells in vitro.     

Relation between TNF-α,TNFR I expression and apoptosis in oral lichen planus

AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus.     

Osmolarity,cell volume and proliferation in nasopharyngeal carcinoma cells

AIM: To investigate the relationship between osmolarity, cell volume and cell proliferation in nasopharyngeal carcinoma cells. METHODS: MTT method was applied to detect the proliferation ability of the poorly-differentiated nasopharyngeal carcinoma cell (CNE-2Z) under various osmolarity conditions. The flow cytometry was used to analyse cell cycle distribution. Cell volume was obtained by the image analysis of living cells and cell viability was determined by the trypan blue assay. RESULTS: Cultivation of cells under the hypertonic conditions of 370 and 440 mOsmol/L increased cell volume by 8.7% and 27.8% and facilitated cell proliferation by 22.2% and 33.9%, respectively. However, hypotonic incubation of cells with osmolarity of 160 and 230 mOsmol/L decreased cell volume by 12.8% and 4.1% and inhibited cell proliferation by 34.0% and 15.6%, respectively. Cell volume was positively correlated with cell proliferation rate. Long-term cultivation of cells under anisotonic conditions did not significantly alter cell cycle distribution, but hypotonic cultivation decreased cell viability. CONCLUSION: Proliferation of nasopharyngeal carcinoma cells was closely correlated with the osmolarity of culture medium and cell volume. Hypotonic cultivation may inhibit cell proliferation by decreasing cell volume to facilitate cell death mechanisms.     

Effects of component of some Chinese herbs on proliferation of human umbilical vein endothelial cells in vitro

AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragaluspolysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P<0.05 or P＜0.01), and Rg1 inhibited proliferation of HUVEC at 31 mg/L (P<0.05); VAP promoted proliferation of HUVEC at 1 mg/L-0.5 g/L with optimal dose of 10 mg/L (P<0.01), Cinnamyl aldehyde promoted proliferation of HUVEC at 2 g/L(P＜0. 05); Muscone and soluble extract of BCB inhibited proliferation of HUVEC at 1 g/L, 0.5-2.5 kg/L(P＜0. 01), respectively; Perlolyrine inhibited proliferation of HUVEC at 0.125 g/L-0.5 g/L(P＜0. 01). CONCLUSION: The external herbs for supplementing Qi and warming Yang can promote HUVEC proliferation and improve angiogenesis during tissue repair. The external herbs for promoting blood circulation and accelerating capillary movement may have influence upon other stages of tissue repair.     

Effects of fosinopril,captopril and valsartan on the expressionof tissue factor in human monocytes

AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT₁ antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced.     

野大麦、羊草的光合和蒸腾作用特性比较及利用方式的研究

在外界适宜的环境条件下设置13个光照梯度,对人工草地上的一年生羊草和一年生野大麦植株叶片进行光模拟实验。实验证明,光照强度的改变对这两种植物叶片的叶温、气孔导度、胞间CO2浓度、蒸腾作用及光合作用等生理指标存在不同的影响。野大麦植株的叶温始终比羊草低,在高光强照射下具有更强的调节叶温的能力,两者叶温最大差值为1.24℃;与羊草相比,光强对野大麦叶片的气孔运动影响较大,其气孔导度与胞间CO2浓度显著高于羊草。野大麦光合速率、日间暗呼吸、蒸腾速率强,用水效率值(WUEph)低。依据测量数据及其它相关指标,提出生产实践中野大麦适于放牧利用,而羊草适于割草利用。     

三肽囊素对环磷酰胺诱导的鸡红细胞免疫功能低下的阻断作用

本文研究了三肽囊素对环磷酰胺诱导的墟岗黄鸡红细胞免疫功能的抑制模型的影响。180只一日龄墟岗黄母鸡随机分成对照组,三肽囊素组,三肽囊素 环磷酰胺组,环磷酰胺组四个组,三肽囊素组和环磷酰胺 三肽囊素组每只鸡肌注0.01mg/kg剂量的三肽囊素;环磷酰胺组和环磷酰胺 三肽囊素组每只鸡肌注40mg/kg剂量的环磷酰胺,对照组鸡注射等量的生理盐水。结果表明,14日龄和35日龄时各试验组鸡的红细胞总数没有显著差异,而在56日龄时,环磷酰胺组显著低于其它三组(P<0.05);从14日龄开始,环磷酰胺纽的RBC-C3bR花环率显著低于其它三组,而环磷酰胺组的RBC-IC花环率显著高于其它三组,并持续到实验结束。结果提示,三肽囊素可阻断由环磷酰胺诱导的鸡红细胞免疫功能低下。     

蜱传性疾病--莱姆病螺旋体研究进展

莱姆病是一种蜱传性人兽共患病,在我国北方各省广泛流行,其病原体为伯氏疏螺旋体。莱姆病可使皮肤、心脏、关节和神经系统等多种组织器官受损。本文综述了莱姆病螺旋体的形态、种下分类、生化特征、传播媒介和贮存宿主等方面的研究进展"