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本研究对云南地区近五年来牛冷冻精液主要质量性状(活力、前进运动精子数、畸形率)进行了检验分析,随机抽取样品455头份,均为云南企业生产的产品,涉及品种有荷斯坦、西门塔尔、安格斯、短角、摩拉、尼里、槟榔江等。应用统计学原理对相关数据进行分析,结果表明:解冻后平均活力为42.0%、每剂量中前进运动精子数为1481万个、畸形率16.3%。品种间比较,活力差异均不显著(P>0.05)。前进运动精子数,肉用牛和水牛均显著高于荷斯坦牛(P<0.05)。畸形率方面,水牛分别高于荷斯坦和肉用牛1.4、1.6个百分点(P<0.05)。不同生产单位间比较,B站水牛、肉用牛的活力分别高于A站1.9、1.3个百分点(P<0.05)。B站水牛前进运动精子数高于A站96万个(P<0.05)。B站畸形率低于A站1.2个百分点(P<0.05)。 相似文献
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LIU Cui-cui GUO Xiao-hong CAO Guo-qing GAO Peng-fei PU Zhong-de NIU Jiao-yan ZHANG Qi LI Meng JIN Yu-shu LI Bu-gao 《中国畜牧兽医》2015,42(10):2726-2731
The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs. 相似文献
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知识管理与虚拟学习具有相关性与互补性,高校图书馆信息共享空间为知识管理与虚拟学习社区的融合创造了有利条件,因此知识管理与虚拟学习社区的融合是可行的,但是面临来自于观念、技术、功能、内容、交互性等方面的障碍。图书馆应尽可能地采用现代信息技术将知识管理系统与虚拟学习社区集成在一个IC内,进而提升自身的服务水平。 相似文献
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建立双波长高效液相色谱法(HPLC)同时测定模拟氮沉降处理下杜仲Eucommia uimoides皮和叶中松脂醇二葡萄糖苷、桃叶珊瑚苷、绿原酸、京尼平苷酸和京尼平苷等5种活性成分含量的方法。采用Agilent ZORBAX SB-C18柱(250.0 mm×4.6 mm,5μm),以乙腈(A)-2.0 g·kg-1磷酸水溶液(B)进行梯度洗脱(0~12 min,10%A;12~25 min,13%A;25~50 min,30%A;50~65 min,60%A);流速为0.8 m L·min-1;检测波长203 nm,240 nm;柱温30℃。结果表明:松脂醇二葡萄糖苷、桃叶珊瑚苷、绿原酸、京尼平苷酸、京尼平苷等在各自的线性范围内与其峰面积积分值呈良好的线性关系。整个实验期间,杜仲皮和叶中5种成分含量对施氮处理产生了不同的响应,且随季节在变动。该方法能准确、快速地对杜仲皮和叶中多种有效成分同时进行测定,可较好反映浙江高氮沉降区特色名贵药材杜仲皮和叶中多种有效成分的变化。 相似文献
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AIM: To explore the effects and mechanism of eleutheroside (ETS) B or E on the proliferation of HBZY-1 cells treated with high glucose. METHODS: The HBZY-1 cells were cultured under high glucose condition. The 4th generation of HBZY-1 cells was used for determining the optimal cell density, which was consistent with the growth regulation curve of the cells. The cells were divided into 6 groups: low glucose (LG) group, high glucose (HG) group, high glucose plus ETS-B/E (low dose, medium dose and high dose) groups, and high glucose plus losartan (LTG) group. After all cells were treated with the corresponding drugs at 24 h, 48 h and 72 h, the inhibitory rate of the proliferation was measured, and the expression of TGF-β1 and PPARγ was detected by immunocytochemistry and Western blotting. RESULTS: The best cell density was 2 000 cells/well, which was complied with the basic rules of the cell growth, and high glucose significantly promoted the HBZY-1 cell proliferation. At each time point, the inhibitory effects of ETS-B/E were significantly different between HG group and LTG group on the proliferation of the HBZY-1 cells (P<0.05). The expression of TGF-β1 was significantly inhibited, and the expression of PPARγ was significantly promoted by ETS-B/E (P<0.05). ETS-E showed stronger effect than ETS-B (P<0.05) in a concentration- and time-dependent manner. CONCLUSION: ETS-B/E significantly inhibits the proliferation of HBZY-1 cells under high glucose condition by decreasing TGF-β1 expression and promoting PPARγ expression. 相似文献