Electrolyte leakage of aging potato tubers and its relationship with sprouting capacity

Summary With advancing physiological age of potato tubers an increase in electrolyte leakage from tuber tissue was observed for various seed lots. This increase was most marked after tubers exhibited sprout growth and corresponded with a decline in tuber water content. Leakage increased minimally with storage time when sprouting was prevented or limited by either dormancy, low storage temperature, or frequent desprouting. Sprouting capacity of tubers increased to a maximum and then declined with storage time. This decline coincided with the increase in electrolyte leakage, suggesting that membrane integrity was one determinant in vigour of seed potato tubers. The pattern of electrolytes leaking from tuber tissue could be described by a function composed of a saturable and a linear component, suggesting two different sources contributing to the leakage. The saturable component, expected to reflect membrane integrity, was most responsive to aging treatments. H/LA Paper No. 93-13. Department of Horticulture and Landscape Architecture, Project No. 1984. College of Agriculture and Home Economics Research Center, Washington State University. This work was supported in part by grant funding from the Washington State Potato Commission.     

Evaluation of on-farm irrigation scheduling methods for potatoes

The neutron probe, infrared thermometry and crop water stress index (CWSI), and a computer-assisted irrigation scheduling method were evaluated in terms of their effect on tuber yield, tuber quality, and water use. The experiment was conducted during 1990 and 1991 near Othello, central Washington, using Russet Burbank potatoes grown in a silt loam soil. Irrigation treatments did not commence until after tuber initiation. In general, no differences in total number of tubers and total tuber yield resulted from the different scheduling methods. However, the canopy temperature method showed reduction in the yield of number one tubers in 1990. The least total irrigation water was applied during the growing season with the neutron probe method. Using CWSI values above 0.5 to 1.0 (scale 0 to 10) for two consecutive days as a threshold to schedule irrigations appeared to be adequate for potatoes grown in silt loam soils. However, shortcomings of infrared thermometry suggested that this method may not be practical for scheduling irrigation of potatoes.     

Surface hydrophobicity of physicochemically and enzymatically treated milk proteins in relation to techno-functional properties

Surface hydrophobicity (SH) of milk proteins treated physicochemically (by heating and Maillard reaction) or modified enzymatically (by transglutaminase, lactoperoxidase, laccase, and glucose oxidase) was assessed in relation to their techno-functional properties. Heat-treatment increased SH of whey protein isolate and decreased SH of sodium caseinate and bovine serum albumin. Maillard reaction of milk proteins caused time-depended decreases of SH. Only for total milk protein reacting with glucose and lactose elevated SH-values were detected. Protein modification with transglutaminase, laccase, and lactoperoxidase strongly increased the SH of whey protein isolate and total milk protein. Incubation with glucose oxidase elevated SH values of sodium caseinate, whey protein isolate, and total milk protein. When correlating SH with techno-functional properties, a positive correlation was observed between SH and foam formation, and a negative correlation was observed between SH and foam stability as well as emulsion stability. No clear correlation was detected between SH and emulsifying activity, surface tension, viscosity, and heat stability of enzymatically and physicochemically treated milk proteins.     

Food safety and residues in Australian agricultural produce

SUMMARY A brief history of food safety in Australia in the context of the development of scientific knowledge is presented. Australia's food and food commodity residue and contamination surveillance and monitoring programs are outlined. Although chemical residues are perceived as a major health risk by the general community, the risk of food causing illness or death because of chemical residues is low. The major threat to human health from food is microbiological contamination, in terms of deaths, sickness and economic loss. The emerging influences in food safety are scientific and technical developments, deregulation, social and demographic factors, and the media. The risk of disruption of domestic and export markets by food safety issues is considerable. The emergence of international standards for food production and processing will enable commercial contractual arrangements to minimise the frequency of disruptive food safety incidents.     

Solution structure of the integral human membrane protein VDAC-1 in detergent micelles

The voltage-dependent anion channel (VDAC) mediates trafficking of small molecules and ions across the eukaryotic outer mitochondrial membrane. VDAC also interacts with antiapoptotic proteins from the Bcl-2 family, and this interaction inhibits release of apoptogenic proteins from the mitochondrion. We present the nuclear magnetic resonance (NMR) solution structure of recombinant human VDAC-1 reconstituted in detergent micelles. It forms a 19-stranded beta barrel with the first and last strand parallel. The hydrophobic outside perimeter of the barrel is covered by detergent molecules in a beltlike fashion. In the presence of cholesterol, recombinant VDAC-1 can form voltage-gated channels in phospholipid bilayers similar to those of the native protein. NMR measurements revealed the binding sites of VDAC-1 for the Bcl-2 protein Bcl-x(L), for reduced beta-nicotinamide adenine dinucleotide, and for cholesterol. Bcl-x(L) interacts with the VDAC barrel laterally at strands 17 and 18.     

Allgemeine Merkmale sowie Blei- und Zinkmobilität in urban-industriell überformten Böden des Brücktorviertels in Oberhausen (Ruhrgebiet)

General characteristics as well as the mobility of lead and zinc in soils which were transformed by urban and industrial impact of the Brücktor district in Oberhausen (Ruhr area) This paper presents the results of Urbic Anthrosols from eight different sites which contain partly alkalising material of technological origin in an urban-industrial area in Oberhausen. These results were then compared with those of tests taken of a nearby Cambisol from Oberhausen. The pH-value of the profiles with the deposit layers are almost always in the alkaline range (highest pH = 9,8). More than 50 % of the deposit layers of the tested soils show a coarse texture with a high to very high content of gravel, ashes, cinders, clinker and mining waste. The soils are deep enriched with fossilized and pyrolytic transformed carbon substances which are recorded, as well as the recent humus, by using the Lichterfelder-method to determine the Corg. From the substratum of the deposit layers Na, K, Mg and Ca were moved into deeper horizons which could cause an increase in the pH-value there. The eight tested soils partly show a high accumulation of heavy metals (up to 4,8 g Pb/kg, up to 2,8 g Zn/kg). High heavy metal contents were measured in the deposit layers of ashes. Unlike Na, K, Mg and Ca, it could not be shown that a transfer of mobile Zn and Pb into deeper layers of soil takes place. In spite of a lower background pollution, the acidified Cambisol contains, in comparison to the eight tested soils, a clearly higher mobile Zn-content which could be transferred through the profile.     

The dependence of internal brown spot development upon calcium fertility in potato tubers

Summary To determine calcium fertility and high temperature effects on internal brown spot development in potato, cv. Russet Burbank plants were fertilised with Hoagland's nutrient solution with or without calcium. Prevalence and severity of internal brown spot were greatest in tubers receiving no calcium fertilisation during growth. Internal brown spot was evident as early as tuber initiation. A high growth medium temperature (32°C) at tuber maturity with a lack of calcium increased internal brown spot incidence and severity. No tubers subjected to the high temperature and fertilised with calcium developed internal brown spot. Peel calcium concentration of tubers receiving no calcium fertilisation was lower (0.5–0.8 g/kg) than tubers receiving calcium (1.2–1.9 g/kg). Medullary tissue calcium concentration was similar under both calcium regimes. Calcium availability during early tuber growth was an influential factor in internal brown spot development, however high temperature effects may not be discounted in exacerbating the disorder. H/LA Paper No. 95-7. Project No. 1984, College of Agriculture and Home Economics Research Center, Pullman WA 99164, USA     

Tibial tuberosity advancement in 92 canine stifles: initial results,clinical outcome and owner evaluation

Objective To investigate the clinical outcomes, complications and owners' evaluation of the tibial tuberosity advancement (TTA) procedure in canine stifles. Methods A retrospective study of hospital records was performed to identify dogs diagnosed with partial or complete cranial cruciate ligament (CCL) rupture that had undergone TTA repair. Information obtained included signalment, period of lameness, surgical report, evidence of meniscal injury, postoperative recovery and peri-operative complications. Owners were asked to assess the long-term outcome. Results In a total of 72 dogs (median age, 6 years; median body weight, 34.8 kg), TTA was performed in 92 stifles. Twenty breeds were represented, with Labrador Retrievers and Rottweilers the most common. The period of lameness ranged from 3 days to 24 months. The median pre-operative lameness score was 3/4 and meniscal injury was present in 51 stifles. Minor complications occurred in 29% of cases. Major complications occurred in 6.5% of cases and consisted of meniscal injury and two tibial tuberosity fractures. All were successfully managed, with good limb function when subsequently assessed. In the owner evaluation, 96% reported moderate to great improvement postoperatively, with no lameness at rest and mild to no lameness after vigorous exercise. Conclusion Clinical outcome and owner evaluations in this case series indicate favourable results can be expected when CCL-deficient stifles are treated with TTA.     

Programs for surveillance and monitoring of antibacterial residues in Australia, 1989 to 1993

SUMMARY The development of a State-based confirmatory testing capability for antibiotic residues in meat in Australia has allowed the rapid feedback to producers failing to comply with antibiotic maximum residue limits. The identification of problem areas in various categories of livestock, and subsequent focused surveillance programs, has reduced the prevalence of antibacterial residues in both domestic and export meat products. Failure to observe withholding periods of antibacterial drugs after treatment is the most significant cause of non-compliance. In the period July 1991 to June 1993 the compliance rate for antibacterial residues for all species was 99.9%.