Chloramphenicol concentrations in calf muscle tissue

Twenty-five 9- to 11-week-old calves were administered 2 doses of chloramphenicol prepared in propylene glycol (13.6 mg/kg of body weight IV; 6.8 mg/kg IM; or 13.6 mg/kg IM) at 24-hour intervals. Calves were euthanatized at designated times from 2 to 72 hours after the last dose was administered. Muscle tissues were collected immediately after euthanasia, and chloramphenicol concentrations in the tissues were determined.     

HTLV-III infection in brains of children and adults with AIDS encephalopathy

Unexplained debilitating dementia or encephalopathy occurs frequently in adults and children with the acquired immune deficiency syndrome (AIDS). Brains from 15 individuals with AIDS and encephalopathy were examined by Southern analysis and in situ hybridization for the presence of human T-cell leukemia (lymphotropic) virus type III (HTLV-III), the virus believed to be the causative agent of AIDS. HTLV-III DNA was detected in the brains of five patients, and viral-specific RNA was detected in four of these. In view of these findings and the recent demonstration of morphologic and genetic relatedness between HTLV-III and visna virus, a lentivirus that causes a chronic degenerative neurologic disease in sheep, HTLV-III should be evaluated further as a possible cause of AIDS encephalopathy.     

Showering as a coolant for rats exposed to heat

Rats exposed to heat learn to take showers and thus defend themselves against hyperthermia; thereafter the rate of showering is a direct function of ambient temperature. The showering effectively replaces spreading of saliva as the major defense against heat in desalivate rats, and appears to be highly preferred to the use of saliva by the intact animal. Since rats will work to obtain heat when cold, it is now clear that learned behavior is effective and physiologically appropriate for thermoregulation at temperatures both above and below the neutral range.     

A comparison of anesthetic risk factors and outcomes in light and draft horses

As a result of anatomic and physiologic differences, draft breeds may be at greater risk of developing anesthetic complications. The aim of the study was to evaluate and compare anesthetic management of draft (DR) and light (LT) horses. A case‐matched retrospective study of 371 clinical case records of DR (124 cases) and LT (247 cases) horses presented for general anesthesia between 1991 and 1998 was performed. Data were tabulated and comparisons were made using Student's t‐test (significance p < 0.05). Prior to induction, there were significant differences in mean body weight, rectal temperature, PCV, RBC, and serum TP concentration between DR and LT breeds. There were differences in mean doses of pre‐operative butorphanol (LT 21 µg kg^?1; DR 17 µg kg^?1), induction guaifenesin (LT 99 mg kg^?1; DR 88 mg kg^?1), and intraoperative ketamine (LT 0.35 mg kg^?1; DR 0.56 mg kg^?1) required. There were no significant differences in the mean doses of pre‐operative xylazine, detomidine, or induction barbiturate administered. The mean, average, and maximum concentrations of inspired halothane were significantly higher for DR than for LT horses. Draft horses received 33% less intraoperative IV fluids (8.2 mL kg^?1 hour^?1) than LT horses. Mean anesthetic duration, time to extubation, and standing recovery were not significantly different. Induction complications were not reported for either group. Rates of occurrence of intraoperative bradycardia, hypercarbia, hypoxemia, and metabolic acidosis (SBE, TCO₂, and bicarbonate concentration) did not differ significantly. Average MAP was greater in DR horses, but neither the degree nor the mean duration of hypotension differed between DR and LT horses. Mean PaO₂ was significantly lower in DR (246 mm Hg, 32.8 kPa) than in LT (305 mm Hg, 40.7 kPa) breeds. Draft horses were at greater relative risk of hypoventilation than LT horses. The greater MAP and requirement for halothane and intraoperative ketamine may indicate problems in achieving and maintaining a surgical plane of anesthesia. Draft horses may be at a greater risk of ventilation–perfusion mismatching.     

Canine parvoviral myocarditis

A 5-week-old crossbred pigdog pup was presented in respiratory distress. It appeared to dry retch, and then died suddenly before a clinical examination was performed; a littermate had died suddenly the week before. Histopathological examination revealed an ongoing lymphocytic myocarditis, characterised by myofibril degeneration and lymphocytic inflammatory infiltrates. In scattered degenerate myofibres, there were large amphophilic intranuclear inclusion bodies distending their nuclei. There was also marked pulmonary oedema. Canine parvoviral myocarditis, due to canine parvovirus type 2, was diagnosed. Further questioning regarding the history of this pup revealed that it had been born to an unvac-cinated dam that had been moved late in her pregnancy from an isolated farm on to another property. It seems likely that the dam was naïve and only became exposed to the virus immediately prior to whelping. The lack of wildlife vectors and a well-controlled population of dogs in rural New Zealand means that there may be isolated areas of the country where dogs can reach maturity without becoming exposed to parvovirus, creating the potential for development of this now uncommon syndrome. When parvovirus first arose in New Zealand, between 1979–;1981, parvoviral myocarditis was relatively common, occurring in neonates when the virus was introduced to breeding kennels that previously had been unexposed.