Evaluation on Tolerance of Montmorillonite as a Mycotoxin Adsorbent

In order to study the tolerance dose of Kunming mice to montmorillonite as a mycotoxin adsorbent in diets,the experiment selected 60 healthy male Kunming mice with initial average body weight of (17±0.12)g,randomly divided them into 5 treatments with two replicates per group and 6 mice per replicate.Five experimental diets were added to 0 (control group),0.5%,1%,2% and 5% montmorillonite,respectively.After 30 days,the growth performance,nutrients apparent digestibility,serum biochemical indices and internal organization were tested to determine the tolerance dose of montmorillonite for Kunming mice.The results showed that the ADFI was significantly increased in 0.5% group (P< 0.05),while there were no significant differences in ADFI and ADG between 1% group and control group (P> 0.05),then the ADFI was significantly decreased with the increasing of montmorillonite supplemental level (P< 0.05).The ADG in 2% group had no significant difference when compared with the control group (P> 0.05),while significantly lower than that in 0.5% and 1% groups (P< 0.05).The apparent digestibility of DM,CP and EE showed no significant effect at any level of montmorillonite (P> 0.05).Serum ALT,AST activities and MDA content were gradually increased with the montmorillonite supplemental level increasing,and that in 1%,2% and 5% groups were significantly higher than that in control group (P< 0.05),but there were no significant differences between 0.5% group and control group (P> 0.05).Tissue sections showed that mice livers were damaged in varying degrees when the montmorillonite supplemental level was 5%.In conclusion,based on a comprehensive consideration of all indexes,it could be seen that the tolerance dose of the tested montmorillonite for Kunming mice was between 0.5% and 2% when mycotoxin contents in diets were in security range.     

基于分段波形的信号瞬时频率计算方法

针对局部均值分解（Local Mean Decomposition，LMD）中乘积函数（Product Function，PF）分量的瞬时频率计算问题，引入了一种新的信号瞬时频率计算方法.该方法基于分段波形，先将信号分成若干个全波段（full wave），然后以一组递增的反正弦函数定义每个全波段的瞬时相位，进而得到信号的瞬时频率.由该方法得到的瞬时频率理论上是正的、稳定的并且能够确保信号局部特征信息的完整.应用该方法计算了仿真信号和实际齿轮故障振动信号的瞬时频率，并与其他方法求得的瞬时频率进行了对比.结果表明，本文方法非常适合求取信号的瞬时频率.     

鱼肠道弧菌免疫检测方法的研究

采用灭活的鱼肠道弧菌作为抗原,制备兔抗血清,建立了鱼肠道弧菌的间接荧光抗体检测技术(indirect im-munofluorescence assay test,IFAT)、Western-blotting免疫印迹检测技术。IFAT结果显示阳性信号覆盖整个菌体,明亮,清晰,荧光信号呈长弧形且两端钝圆,与鱼肠道弧菌形状一致。Western-blotting结果显示共10条蛋白带发生免疫反应,其中6条蛋白带结果较清晰,显示出较强的特异性免疫反应,阴性对照组无条带。IFAT和Western-blotting结果说明所建立检测方法能够准确、快速的检测出鱼肠道弧菌。     

用模糊综合评判法预测马尾松毛虫幼虫高峰期发生量

为了提高马尾松毛虫一代、二代幼虫高峰期发生量预报的准确性,为有效防治马尾松毛虫提供科学依据,本文运用模糊综合评判的6个数学模型预测安徽省潜山县马尾松毛虫一代、二代幼虫高峰期的发生量,验证预报1989年、1994年、2002年和2017年一代马尾松毛虫幼虫高峰期发生量,预报结果分别为2级、4级、2级和1级,与实况级别完全吻合,预报结果准确。预报1989年、1994年、2002年和2017年二代马尾松毛虫幼虫高峰期发生量,预报结果依次是4级、5级、2级和1级。同样与实况级别全部相同。预报的准确率为100%,模糊综合评判法是一个运算简便、准确性高的预报方法。     

基于关联度、主成分和聚类分析的西南区玉米新组合评价

对于玉米育种而言,新组合的科学评价至关重要。以西南区55个玉米新组合为研究材料,对其产量等15个农艺性状进行灰色关联度分析、主成分分析和聚类分析。结果表明:不同玉米新组合农艺性状与产量的关联度大小依次为单株粒重>穗粗>籽粒含水量>百粒重>空杆率>穗行数>出籽率>穗长>倒折率>行粒数>茎粗>株高>穗位高>秃尖长。以产量最高组合1703为理想品种,综合评价表现较优的组合有成单388、绵1308、川单1751和荣玉1756。通过主成分分析将14个性状降为9个主成分,可解释表型变异的91.11%。依据主成分综合评价模型,可将所有组合分为6类,其中,川单1751、青青711、恩1010、成单388、绵1308、青青733和1703表现最优。聚类分析结果则将所有材料分为5类,结果与主成分分析法得出的结论基本一致。结果表明,玉米新组合绵1308、成单388、川单1751和1703表现最优。     

公司治理、管理者动机与衍生金融工具使用

基于中国上市公司2007~2013年财务数据，研究公司治理对管理者使用衍生金融工具的影响，并结合中国制度背景，深入分析和检验企业产权、政策监管对公司治理效应的影响。实证结果表明，公司治理对管理者使用衍生金融工具的动机存在重要影响，公司治理水平越高，管理者越倾向于利用衍生金融工具避免财务困境风险；相反，管理者越倾向于利用衍生金融工具规避薪酬风险。研究还发现，公司治理的作用机制会受到所有权性质的影响，国有控制属性会弱化公司治理效应，对衍生金融工具交易的政策监管差异是重要原因。     

河南新县野生油茶群体表型变异特征研究

为揭示河南新县野生油茶群体的变异特征,以新县7个典型野生油茶生长区的群体为研究对象,测定了油茶叶、花、果相关的15个性状,采用变异系数、相关性分析、聚类分析和方差分析等方法,分析油茶表型性状的多样性及群体间和群体内的变异特征。结果表明,新县野生油茶叶、花、果形态在群体内及群体间的表型多样性均较为丰富,7个群体间叶、花、果形态差异均达到极显著水平。7个群体内15个性状中,鲜籽质量变异程度最高(49.79%),其次是鲜籽数、单果质量和果皮厚,变异程度最低的是叶长(10.67%);聚类分析在欧式平均距离在阈值15处,将新县野生油茶群体分为了两大类,在不同程度上反映了各群体的特征。     

厚皮甜瓜新品种瓜州王子6号

<正>瓜州王子6号全生育期为78~90 d(天),中早熟。果实椭圆形,表皮坚硬,耐贮运性好,果肉浅绿色,肉质细腻稍脆,中心可溶性固形物含量14.5%~18.0%,有清香味,平均单瓜质量2.5 kg,每667 m2产量3 270 kg左右。甘肃省酒泉市甜瓜种植历史已逾2 000 a (年),生产的甜瓜甜如蜜、脆如梨,风味独特。酒泉市农     

当前龙虾产业发展现状及政策建议——基于“龙虾门”事件对盐城龙虾产业后续影响调查

本文作者结合南京＂龙虾门＂事件,对盐城龙虾产业的现状进行了调查研究,并对龙虾产业发展提出一些建议,供养殖者参考。     

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.