当前龙虾产业发展现状及政策建议——基于“龙虾门”事件对盐城龙虾产业后续影响调查

本文作者结合南京＂龙虾门＂事件,对盐城龙虾产业的现状进行了调查研究,并对龙虾产业发展提出一些建议,供养殖者参考。     

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.     

云南白雪茶研究现状及开发利用

云南白雪茶是滇西北高寒地区民间特有的一种长期使用的药物和保健饮品,具有茶文化特征.从云南民间的食用经验来看,白雪茶具有显著的清热、止咳、安神等保健作用,现代药理学研究又证明,白雪茶还具有抗炎、解热、抗疲劳、抗辐射、促进免疫等药理学活性.根据雪茶的研究现状对其植物学特性、化学成分、保健作用作了简要的阐述,为以后的开发利用提供了理论依据.     

防止三层实木复合地板面层开裂的措施

分析了三层实木复合地板在生产和使用过程中面层开裂的原因,并提出了相应的改进措施,为企业改进生产工艺,提高三层实木复合地板产品质量提供参考.     

贵州省三穗县鸭疫里默氏杆菌的分离鉴定、毒力基因检测及耐药性分析

为了解贵州省三穗县鸭疫里默氏杆菌（Riemerella anatipestifer，RA）的流行血清型、毒力和耐药情况，本试验从贵州省三穗县6个规模养鸭场临床疑似RA感染的病鸭体内分离出6株分离菌，对病原菌进行分离鉴定、毒力基因检测和耐药性分析。细菌分离鉴定结果显示，分离菌在巧克力琼脂培养基上长出表面光滑、圆形半透明的滴状菌落；革兰氏染色呈阴性短小杆菌，瑞氏染色呈两极浓染；分离菌均不具运动性，尿素、触酶和氧化酶试验均为阳性，符合RA生化特性，将6株分离菌分别命名为SS-RA1~SS-RA6；6株分离菌的16S rRNA基因序列与NCBI上RA参考菌株的基因序列相似性≥98%，说明6株分离菌均是RA；SS-RA1~SS-RA4为血清2型且含有8种毒力基因（OmpA、CAMP、wza、AS87_04050、Fur、SIP、TbdR1和luxE基因），SS-RA5和SS-RA6为血清11型，缺失AS87_04050基因，仅含有上述其余7种毒力基因；动物回归试验结果显示，攻毒组雏鸭均全部死亡，对照组雏鸭未表现明显临床症状，表明6株分离菌对雏鸭均有致病力；药敏试验结果显示，6株分离菌仅对羧苄西林、哌拉西林、头孢他啶、头孢氨苄、头孢曲松、头孢拉定和头孢哌酮7种抗菌药物敏感，对其他13种抗菌药物均表现不同程度的耐药，对氨基糖苷类和大环内酯类抗菌药物的耐药率为100%。本试验成功分离得到6株RA，为贵州省三穗县鸭疫里默氏杆菌病的疫苗选择和药物防治提供理论依据。     

防护条件下梭梭幼苗生长及养分吸收特性

以新疆古尔班通古特沙漠南缘管件防护与传统移栽2种方式移栽的梭梭幼苗为实验材料,通过野外监测与室内分析,探讨了管件防护条件下梭梭幼苗的生长及养分吸收特性。结果表明:整个生育期内,管件防护梭梭幼苗的各生长指标与传统移栽苗相比,增幅最大的为茎粗,其次为株高。年周期内管件防护梭梭幼苗的生物量呈递增趋势,单株生物量累积可达45.25 g·株~(-1);净增加27.10 g·株~(-1),是传统移栽苗的1.38倍(P0.05)。管件防护梭梭幼苗体内的氮素、磷素、钾素含量呈先升高后降低趋势,在同化枝生长期达到峰值,分别为11.376 g·kg~(-1)、1.066 g·kg~(-1)和23.340 g·kg~(-1),较传统移栽苗高24%、15%和8%,差异显著;而其梭梭幼苗氮素、磷素、钾素的总积累量却呈递增趋势,年净增积累量分别为0.297 g·株~(-1)、0.027 g·株~(-1)和0.560 g·株~(-1),是传统移栽苗的2.23倍、1.72倍和1.65倍;各时期梭梭幼苗体内的氮素、磷素、钾素含量及总积累量均高于传统移栽苗。     

淮安区水稻测土配方施肥配方校正与示范对比试验报告

为验证肥料配方的准确性,发挥配方肥料施用的最大效益。通过田间校验,示范对比测土配方施肥的增产效果,进一步验证并完善淮安区的肥料配方,优化测土配方施肥技术参数。     

3种不同基因型扁豆耐盐性的比较

[目的]比较不同基因型扁豆的耐盐性能.[方法]选用品种春扁豆(Ⅰ)(荚为绿色)、绿领红扁豆(荚为紫色)以及超级扁豆(Ⅰ)(荚为绿白色)为试验材料,在种子萌发期间用不同浓度的NaC1溶液进行盐胁迫,采用加权累积隶属函数法综合评定它们的耐盐性能.[结果]3种不同基因型扁豆开始感受盐胁迫的浓度为0.5％ NaCl,随着NaC1溶液浓度增加,其种子萌发和幼苗生长受抑制程度加大,但在同一浓度NaCl溶液处理下,受抑制程度不同.[结论]3种基因型扁豆耐盐性能依次为:春扁豆(Ⅰ)＞绿领红扁豆＞超级扁豆(Ⅰ).     

橄榄多酚类物质研究进展

橄榄是中国传统的药用植物，其中多酚类物质是橄榄重要的功效成分之一。目前，橄榄多酚类物质研究较为 广泛，常用的提取方法包括温浸法、超声波辅助提取法、微波辅助提取法、超声与微波协同辅助提取法等，分离纯化 后采用液相色谱或液质联用技术鉴定多酚类物质的种类和成分，并对其抗氧化、抗病毒、抗菌消炎、抗癌抑癌、解酒 护肝和提高机体免疫等方面的药理活性进行验证。此外，橄榄多酚类物质合成和代谢分子机理也已经被初步阐释。基 于上述研究报道，本文进一步对橄榄多酚类物质后续研究方向和产业化开发利用前景进行展望。     

Identification and Molecular Characteristics Analysis of Porcine Epidemic Diarrhea Virus Variants

To ascertain a diarrhea case in a pig farm in Shandong province,the pathological changes of dead piglets were observed and nested RT-PCR test was carried out on 7 diarrhea samples for porcine epidemic diarrhea virus (PEDV).Pathological examination revealed that intestine was detected as enlargement,hyperemia and edema.After histological examination,the typical microscopic lesions of intestine were disappearance of epithelial cells,villus shrinkage and shortening.The result of nested RT-PCR showed that all of the 7 samples could amplify a specific target band of PEDV.Molecular characteristics of two field strains showed that they had an amino acid homology of 92.3% to 92.4% with vaccine CV777 and 96.6% to 98.6% with other previous field strains which sequences were downloaded from GenBank.Phylogenetic tree analysis further revealed that all of PEDV strains could be mainly divided into two clusters of G1 and G2. G2 consisted of the field strains of our study and other field strains from USA,China and so on,which had the same sequence characteristics of two insertions and one deletion,while G1 consisted of all vaccines of CV777 and several older field strains from China and Korea.These results indicated that our field strains were the dominant strains in recent epidemic diarrhea occurrence.Moreover,its molecular characteristics might be a characterization of differentiating the field and vaccine strains.